Elsevier

Journal of Structural Biology

Volume 104, Issues 1–3, July–September 1990, Pages 120-127
Journal of Structural Biology

Association between the nucleolus and the coiled body

https://doi.org/10.1016/1047-8477(90)90066-LGet rights and content

Abstract

By means of light and electron microscopic immunocytochemistry, we have localized p80-coilin, a specific protein marker for coiled bodies, in mammalian cell lines as well as in primary rat neuron cultures. p80-coilin-stained nuclear bodies, which also contained fibrillarin, could be subsequently silver stained by a method specific for the visualization of nucleolar organizer regions. In cycling cells, most coiled bodies were not associated with nucleoli, whereas in rat neurons such an association was frequent. The treatment of cycling cells with actinomycin D or 5,6-dichloro-1-β-d-ribo furanosyl-benzimidazole led to nucleolar segregation and/or disintegration, and to an association of p80-coilin staining structures with nucleoli. p80-coilin-positive structures contained fibrillarin in both untreated and treated cells. These results support the opinion that there might be a special association between coiled bodies and nucleoli, particularly in neuronal cells.

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      Citation Excerpt :

      CBs, first reported in neuronal cells by Ramon y Cajal in 1903,2 are subnuclear domains present in cells with relatively high transcriptional demands (i.e., cancer and neurons) and contain locally high concentrations of factors required for small nuclear ribonucleoprotein (snRNP) and telomerase biogenesis.3–7 A number of the proteins that are found concentrated in the CB are also found in other distinct nuclear bodies, such as gems,8,9 histone locus bodies,10 and the nucleolus.11,12 For example, the survival of motor neuron protein, SMN, is a component necessary for proper snRNP biogenesis that localizes to the cytoplasm in addition to the CB and/or gem in the nucleus.13,14

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    This work is dedicated to Professor E. Kellenberger on the occasion of his 70th birthday.

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