Sequence of a human keratin 13 specific cDNA encompassing coil 1B through the 3′ end

doi:10.1016/0006-291X(89)90847-4

Biochemical and Biophysical Research Communications

Volume 162, Issue 3, 15 August 1989, Pages 1522-1527

https://doi.org/10.1016/0006-291X(89)90847-4 Get rights and content

Abstract

An expression library established in λgt11 with cDNA from squamous epithelium of the human upper digestive tract was screened with an antibody raised against keratin 13. A 1.2 kb fragment from the most strongly reacting plaque was sequenced and compared to known type I keratin sequences. The highest degree of homology was detected with the murine 47K type I keratin, which we consider to be the counterpart of human keratin 13. Tryptic peptides of keratin 13 were separated on a HPLC column and one peptide was sequenced. The amino acid sequence obtained supports the identity of the cDNA. An eight codon motif has been tandemly repeated in the C-domain of keratin 13. In spite of substantial divergence by point mutations and deletions, the remaining sequence homologies suggest that the C-domains of both the human keratin 13 and the orthologous murine protein have originated from a common ancestor.

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Synthesis of cytokeratin 13, a component characteristic of internal stratified epithelia, is not induced in human epidermal tumors
1989, Differentiation
Human cytokeratin 13 is one of the most abundant intermediate filament (IF) proteins of many internal stratified epithelia and occurs, at least in certain cell cultures, in an O-glycosylated form binding the lectin, wheat germ agglutinin (WGA). As other groups have reported that, in the mouse, the synthesis of mRNA encoding the 47-kDa cytokeratin corresponding to human cytokeratin 13 is induced in epidermal keratinocytes during malignant transformation, we have examined the synthesis of cytokeratin 13 mRNA and protein in human epidermis and epidermal tumors, using specific cDNA probes and cytokeratin 13 antibodies. We isolated two different cDNA clones from the vulvar carcinoma cell line A-431, in which this protein is abundant: One clone seems to represent the entire mRNA, whereas the other is only a minor component and encodes a truncated cytokeratin 13 lacking most of the carboxy-terminal tail domain, probably a product of alternative, “incorrect” splicing. Comparison of the amino acid sequences with those of other cytokeratins revealed a high degree of conservation with respect to several other human type I cytokeratins, notably cytokeratin 15, and to the murine 47-kDa cytokeratin. When human epidermis and a series of benign and malignant epidermal tumors were examined with these cDNA probes and cytokeratin-13-specific antibodies we did not find an induction of expression in keratinocytes, normal or malignantly transformed, except for some scattered, sparse cytokeratin-13-positive cells and very low levels of cytokeratin 13 mRNA, detectable only with the highly sensitive polymerase chain reaction (PCR). We conclude that the gene(s) encoding cytokeratin 13 are not induced in human keratinocytes during epidermal carcinogenesis, in apparent contrast to reports of murine epidermal tumors, and we discuss possible explanations for this interspecies difference.
Cable-piliated Burkholderia cepacia binds to cytokeratin 13 of epithelial cells
2000, Infection and Immunity

^☆: The nucleotide sequence is available through the EMBL sequence database, accession no. X14640.

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Sequence of a human keratin 13 specific cDNA encompassing coil 1B through the 3′ end☆

Abstract

Cell

Exp. Cell Res

Exp. Cell Res

Gene

J. Invest. Dermatol

J. Biol. Chem

Anal. Biochem

J. Biol. Chem

Ann. Rev. Biochem

Laryng. Rhinol. Otol

Nucleic Acids Res