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The preventive effect of Mori Ramulus on oxidative stress-induced cellular damage in skeletal L6 myoblasts through Nrf2-mediated activation of HO-1

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Abstract

The aim of the present study is to investigate the preventive effect of water extract of Mori Ramulus (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide (H2O2)-treated L6 cells. H2O2-triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H2O2 stimulated cytosolic release of cytochrome c and up-regulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H2O2. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H2O2. Furthermore, MRWE enhanced H2O2-mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H2O2-induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H2O2-induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of Mori Ramulus in medicine.

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Funding

This research was funded by the National Research Foundation of Korea Grant (NRF-2021R1A2C201471711 and 2021R1A2C200954911) and Korea Basic Science Institute Grant funded (2020R1A6C101A201)

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Authors and Affiliations

  1. Division of Basic Sciences, College of Liberal Studies, Dong-eui University, Busan, 47340, Republic of Korea

    Cheol Park

  2. Anti-Aging Research Center, Dong-eui University, Busan, 47340, Republic of Korea

    Hyesook Lee & Yung Hyun Choi

  3. Department of Convergence Medicine, Pusan National University School of Medicine, Yangsan, 50612, Republic of Korea

    Hyesook Lee

  4. Department of Food and Nutrition, College of Life and Health, Kyungsung University, Busan, 48434, Republic of Korea

    Sung Ok Kim

  5. Biopharmaceutical Engineering Major, Division of Applied Bioengineering, College of Engineering, Dong-eui University, Busan, 47340, Republic of Korea

    Eun-Woo Lee, Hyun-Tai Lee, Hyun Ju Kwon & Byung Woo Kim

  6. Core-Facility Center for Tissue Regeneration, Dong-eui University, Busan, 47340, Republic of Korea

    Eun-Woo Lee, Hyun-Tai Lee, Hyun Ju Kwon, Byung Woo Kim & Yung Hyun Choi

  7. Department of Marine Life Science, Jeju National University, Jeju, 63243, Republic of Korea

    Gi-Young Kim

  8. Department of Pharmacology, College of Korean Medicine, Daegu Haany University, Daegu, 42158, Republic of Korea

    Mi Ryeo Kim

  9. Department of Biochemistry, College of Korean Medicine, Dong-eui University, Busan, 47227, Republic of Korea

    Yung Hyun Choi

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  1. Cheol Park
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Park, C., Lee, H., Kim, S.O. et al. The preventive effect of Mori Ramulus on oxidative stress-induced cellular damage in skeletal L6 myoblasts through Nrf2-mediated activation of HO-1. Toxicol Res. 39, 25–36 (2023). https://doi.org/10.1007/s43188-022-00141-5

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