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Detection of herpesviruses in neotropical primates from São Paulo, Brazil

  • Veterinary Microbiology - Research Paper
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Abstract

Transmission of herpesvirus between humans and non-human primates represents a serious potential threat to human health and endangered species conservation. This study aimed to identify herpesvirus genomes in samples of neotropical primates (NTPs) in the state of São Paulo, Brazil. A total of 242 NTPs, including Callithrix sp., Alouatta sp., Sapajus sp., and Callicebus sp., were evaluated by pan-herpesvirus polymerase chain reaction (PCR) and sequencing. Sixty-two (25.6%) samples containing genome segments representative of members of the family Herpesviridae, including 16.1% for Callitrichine gammaherpesvirus 3, 6.1% for Human alphaherpesvirus 1, 2.1% for Alouatta macconnelli cytomegalovirus, and 0.83% for Cebus albifrons lymphocryptovirus 1. No co-infections were detected. The detection of herpesvirus genomes was significantly higher among adult animals (p = 0.033) and those kept under human care (p = 0.008671). These findings confirm the importance of monitoring the occurrence of herpesviruses in NTP populations in epizootic events.

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Acknowledgements

The authors thank all the professionals directly or indirectly involved in the Non-Human Primates Epizootic Events Surveillance Program of the Brazilian Ministry of Health in São Paulo State; field, surveillance, and laboratory staff, especially those from the Centro de Vigilância Epidemiológica Prof. Alexandre Vranjac, the Pathology Center, and the Strategic Laboratory of Instituto Adolfo Lutz.

Funding

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (process number 404510/2021) to J.M.G. and the Scientific Initiation Scholarship – PIBIC (CNPq) (#116603/2022) for INF and CSA, Grupo de Apoio às Políticas de Prevenção e Proteção à Saúde/Fundo Especial de Saúde para Imunização em Massa e Controle de Doenças (GAPS/FESIMA numbers 040/2019, 28/2020 and 54/2022), the multi-user sequencing equipment acquired through PDIP—São Paulo Research Foundation (FAPESP) (grant# 2017/50333–7), and the FAPESP research grant 2020/14786–0 to A.L. JLCD is a research fellow of CNPq (grant # 304106/2022).

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Authors and Affiliations

  1. Centro de Patologia, Instituto Adolfo Lutz, Avenida Dr. Arnaldo, 351, Pacaembú, São Paulo, SP, 01246000, Brazil

    Isabella Naomi Furusato, Ketlyn Bolsachini Figueiredo, Ana Carolina Souza Ramos de Carvalho, Camila Santos da Silva Ferreira, Juliana Possatto Fernandes Takahashi, Lidia Midori Kimura, Camila Siqueira Aleixo, Odília Pereira de Brito, Natália Coelho Couto de Azevedo Fernandes, Leonardo José Tadeu de Araújo & Juliana Mariotti Guerra

  2. Programa de Pós-Graduação Em Doenças Infecciosas E Parasitárias - Faculdade de Medicina, Universidade Federal de Mato Grosso Do Sul, Bairro Universitário, Av. Costa E Silva, S/nº, Campo Grande, MS, 79070900, Brazil

    Juliana Possatto Fernandes Takahashi

  3. Centro de Virologia, Instituto Adolfo Lutz, Avenida Dr. Arnaldo, 351, Pacaembú, São Paulo, SP, 01246000, Brazil

    Adriana Luchs & Mariana Sequetin Cunha

  4. Laboratório de Patologia Comparada (LAPCOM), Departamento de Patologia, Faculdade de Veterinária E Zootecnia, Universidade de São Paulo, Avenida Professor Orlando Marques de Paiva, 70, São Paulo, SP, 05508270, Brazil

    José Luiz Catão-Dias & Juliana Mariotti Guerra

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Contributions

INF, CSA, OPB, LMD, JPFT: collected and analyzed the data. MSC: carried out the genetic material extraction. ACSRC, KBF, CSSF, OPB: performed the PCR tests. AL: conduct the sequencing. LJTA: conduct the phylogenetic and mapping analyses. JMG, INF: analyzed, interpreted the data, and drafted the manuscript. JMG, NCCA, JLCD: elaborated the study design. JLCD: supervised JMG in the post-doctoral. All the authors critically revised the manuscript for intellectual content and approved the final version.

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Correspondence to Juliana Mariotti Guerra.

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Furusato, I.N., Figueiredo, K.B., de Carvalho, A.C.S.R. et al. Detection of herpesviruses in neotropical primates from São Paulo, Brazil. Braz J Microbiol 54, 3201–3209 (2023). https://doi.org/10.1007/s42770-023-01105-z

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