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A chloroacetamide derivative as a potent candidate for fusariosis treatment

  • Clinical Microbiology - Research Paper
  • Published:
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Abstract

Fusariosis has presented a significant increase in their incidence in the last years. This epidemiological panorama probably is due to the increasing profile of refractory susceptibility of Fusarium spp. to available drugs, especially in immunocompromised individuals. Thus, the development of new compounds with effectiveness on these organisms is a necessity. This study evaluated the antifungal potential of a chloroacetamide derivative (4-BFCA) against resistant Fusarium strains. As a result, the compound was effective against all strains (MIC range 12.5–50 μg/mL). The time kill assay demonstrated that 4-BFCA presents a concentration-dependent fungicidal action. Although its action mechanism has not yet been elucidated, it was possible to observe its efficacy through damages and alterations provoked along the hyphae of Fusarium spp. 4-BFCA maintained a high survival rate of Tenebrio molitor larvae, suggesting that it does not cause acute systemic toxicity on this host at the concentration evaluated. In addition, 4-BFCA was 83.33% effective in combating a fungal infection in vivo on the chorioallantoid membrane of embryonated eggs. Our results are very promising and arouse interest to investigate the action of 4-BFCA on Fusarium strains since it acts as a possible candidate for the development of new therapies for the treatment of fusariosis.

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Acknowledgements

The authors thank the Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS – EDITAL 04/2016 – PRONUPEQ 2016) for financial support and research fellowships. Alexandre Meneghello Fuentefria is grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the PQ fellowships (Edital Universal 2018).

Author information

Authors and Affiliations

  1. Graduate Program in Agricultural Microbiology and Environment, Federal University of Rio Grande Do Sul, Sarmento Leite no 500 - Farroupilha, Porto Alegre, RS, 90050-170, Brazil

    Gabriella da Rosa Monte Machado, Saulo Fernandes de Andrade & Alexandre Meneghello Fuentefria

  2. Laboratório de Pesquisa Em Micologia Aplicada, Faculdade de Farmácia, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, Brazil

    Gabriella da Rosa Monte Machado

  3. Center for Biological and Health Sciences, Federal University of Oeste da Bahia, Rua da Prainha no 1326 Morada Nobre, Barreiras, BA, 47810-047, Brazil

    Stefânia Neiva Lavorato

  4. Biotechnology Center, Federal University of Rio Grande Do Sul, Bento Gonçalves no 9500 – Campus do Vale, Porto Alegre, RS, 91501-970, Brazil

    William Lopes & Marilene Henning Vainstein

  5. Pharmacology Laboratory, Federal Catarinense Institute, Highway SC 283 - Fragosos, Campus Concórdia, Concórdia, SC, 89703-720, Brazil

    Mário Lettieri Teixeira

  6. Department of Pharmaceutical Products, Faculty of Pharmacy, Federal University of Minas Gerais, Presidente Antônio Carlos no 6627 - Campus Pampulha, Belo Horizonte, Belo Horizonte, MG, 31270-901, Brazil

    Ricardo José Alves

Authors
  1. Gabriella da Rosa Monte Machado
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  2. Stefânia Neiva Lavorato
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  3. William Lopes
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  4. Marilene Henning Vainstein
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  5. Mário Lettieri Teixeira
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  6. Ricardo José Alves
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  7. Saulo Fernandes de Andrade
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  8. Alexandre Meneghello Fuentefria
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Contributions

Gabriella da Rosa Monte Machado participated in the conceptualization, methodology, software, validation, formal analysis, investigation, data curation, writing—original draft preparation, and writing—review and editing of the text. Stefânia Neiva Lavorato participated in the methodology, validation, formal analysis, and data investigation. William Lopes and Mário Teixeira participated in the methodology, data curation, and formal analysis. The professors Marilene Henning Vainstein, Ricardo José Alves, Saulo Fernandes de Andrade, and Alexandre Meneghello Fuentefria participated in the visualization, supervision, project administration, and acquisition of the financial support.

Corresponding author

Correspondence to Gabriella da Rosa Monte Machado.

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da Rosa Monte Machado, G., Neiva Lavorato, S., Lopes, W. et al. A chloroacetamide derivative as a potent candidate for fusariosis treatment. Braz J Microbiol 53, 1289–1295 (2022). https://doi.org/10.1007/s42770-022-00771-9

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  • DOI: https://doi.org/10.1007/s42770-022-00771-9

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