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Antifungal activity of Copaíba resin oil in solution and nanoemulsion against Paracoccidioides spp.

  • Biotechnology and Industrial Microbiology - Research Paper
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Abstract

Paracoccidioidomycosis (PCM) is a disease caused by fungi of the genus Paracoccidioides. The disease is responsible for high rates of premature deaths and socioeconomic repercussions. The limitations of antifungal agents against PCM have motivated the search for new compounds. In our ongoing exploration of Cerrado plants as potential sources of new antifungal agents, we selected Copaifera langsdorffii oil (Copaíba resin oil) in order to explore its bioactive potential and test a formulation to increase oil stability and solubilization employing Pluronic F-127 to obtain the nanoemulsion of the oil. We aim at testing both Copaíba resin oil and its nanoemulsion against four species of the Paracoccidioides genus. We performed cytotoxicity test in Balb/C3T3 cells, hemolytic activity and interaction of Copaíba resin oil and Copaíba resin oil nanoemulsion (CopaPlu) with the antifungal agents such as amphotericin B, co-trimoxazole, and itraconazole. Moreover, the Copaíba resin oil was analyzed by mass spectrometry to identify its chemical profile. Eventually, a new methodology to prepare the nanoemulsion is presented. The Copaíba resin oil and CopaPlu nanoemulsion inhibited Paracoccidioides sp. growth efficiently, and no cytotoxicity or hemolytic effect was observed at minimum inhibitory concentration (MIC). When combined with amphotericin B, Copaíba resin oil and its nanoemulsion showed an additive effect with reduction of MIC values. The Copaíba resin oil and CopaPlu nanoemulsion is a promising antifungal agent against Paracoccidioides.

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Funding

This work was performed at Universidade Federal de Goiás supported by MCTI/CNPq (Ministério da Ciência e Tecnologia/Conselho Nacional de Desenvolvimento Científico e Tecnológico), FNDCT (Fundo Nacional de Desenvolvimento Científico e Tecnológico), FAPEG (Fundação de Amparo à Pesquisa do Estado de Goiás), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/ Finance Code 001), FINEP (Financiadora de Estudos e Projetos), PRONEX (Programa de Apoio a Núcleos de Excelência) and INCT-IF (Instituto Nacional de Ciência e Tecnologia para Inovação Farmacêutica). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, 74690-900, Brazil

    Lívia do Carmo Silva, Meire Ane Costa Miguel Miranda, Célia Maria de Almeida Soares & Maristela Pereira

  2. Laboratory of Colloids and Nanostructured Materials, Institute of Chemistry, Federal University of Goiás, Goiânia, Goiás, Brazil

    Julianna Veiga de Freitas, Sarah Fernanda Araújo Ferreira & Emília Celma de Oliveira Lima

  3. Laboratory of Natural Products, Institute of Chemistry, Federal University of Goiás, Goiânia, Goiás, Brazil

    Cecilia Maria Alves de Oliveira, Lucilia Kato & Ana Paula Terezan

  4. Laboratory of Nanobiotechnology, Federal University of Acre, Rio Branco, Acre, Brazil

    Anselmo Fortunato Ruiz Rodriguez & Fernando Sergio Escócio Drumond Viana Faria

Authors
  1. Lívia do Carmo Silva
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  2. Meire Ane Costa Miguel Miranda
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  3. Julianna Veiga de Freitas
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  4. Sarah Fernanda Araújo Ferreira
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  5. Emília Celma de Oliveira Lima
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  6. Cecilia Maria Alves de Oliveira
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  7. Lucilia Kato
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  8. Ana Paula Terezan
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  9. Anselmo Fortunato Ruiz Rodriguez
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  10. Fernando Sergio Escócio Drumond Viana Faria
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  11. Célia Maria de Almeida Soares
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  12. Maristela Pereira
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Contributions

Each author has contributed significantly to this work. FSE and AFR performed the extraction and identification of Copaíba resin oil. LCS and MACM performed biological analysis and share the first authorship. JVF, SFAF, and ECOL performed the preparation and analysis of the nanoemulsion Copaíba resin. LK, APT, and CMAO performed the characterization of the components of the Copaíba resin oil, and LCS, MACM, CMAS, MP, CMAO, and LK analyzed the data and wrote the paper.

Corresponding author

Correspondence to Maristela Pereira.

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do Carmo Silva, L., Miranda, M.A.C.M., de Freitas, J.V. et al. Antifungal activity of Copaíba resin oil in solution and nanoemulsion against Paracoccidioides spp.. Braz J Microbiol 51, 125–134 (2020). https://doi.org/10.1007/s42770-019-00201-3

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