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Molecular identification and antifungal susceptibility testing of Pucciniomycotina red yeast clinical isolates from Rio de Janeiro, Brazil

  • Bacterial Fungal and Virus Molecular Biology - Short Communication
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Abstract

Infections caused by Rhodotorula spp. are increasing worldwide. This study identified, through the light of the new taxonomic advances on the subphylum Pucciniomycotina, 16 isolates from blood cultures and compared their antifungal susceptibility on microdilution and gradient diffusion methods. Internal transcriber spacer sequencing identified Rhodotorula mucilaginosa (n = 12), Rhodotorula toruloides (n = 2), Rhodotorula dairenensis (n = 1), and Cystobasidium minutum (n = 1). Amphotericin B was the most effective drug. A good essential agreement was observed on MIC values of amphotericin B and voriconazole determined by the two methods. Therefore, the gradient method is useful for susceptibility tests of R. mucilaginosa against these drugs.

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Acknowledgments

We are grateful for the contribution of the Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, and to the students who helped in the mycological analyses.

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Authors and Affiliations

  1. Mycology Laboratory, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, FIOCRUZ, Av. Brazil 4365, Manguinhos, Rio de Janeiro, RJ, 21045-900, Brazil

    Fabio Brito-Santos, Maria Helena Galdino Figueiredo-Carvalho, Rowena Alves Coelho, Jean Carlos Almeida de Oliveira, Raissa Vieira Monteiro & Rodrigo Almeida-Paes

  2. Clinical Pathology Laboratory, HCI, National Cancer Institute, Rio de Janeiro, Brazil

    Alessandra Leal da Silva Chaves

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  1. Fabio Brito-Santos
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  2. Maria Helena Galdino Figueiredo-Carvalho
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  3. Rowena Alves Coelho
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  4. Jean Carlos Almeida de Oliveira
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  5. Raissa Vieira Monteiro
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  6. Alessandra Leal da Silva Chaves
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  7. Rodrigo Almeida-Paes
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Correspondence to Fabio Brito-Santos.

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Brito-Santos, F., Figueiredo-Carvalho, M.H.G., Coelho, R.A. et al. Molecular identification and antifungal susceptibility testing of Pucciniomycotina red yeast clinical isolates from Rio de Janeiro, Brazil. Braz J Microbiol 51, 95–98 (2020). https://doi.org/10.1007/s42770-019-00191-2

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  • DOI: https://doi.org/10.1007/s42770-019-00191-2

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