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Biochemical dynamics during plant pathogen interaction and its potential as disease screening index in Cercospora canescens–mung bean interaction

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Abstract

To explore effective physiological/biochemical traits for screening of mung bean resistance to Cercospora Leaf Spot (CLS) disease, against Cercospora canescens, the effect of biotic stress on antioxidant enzymes [superoxide dismutase (SOD), peroxidase (POX), catalase (CAT)] activities, Phenylalanine ammonia lyase (PAL) activity and lipid peroxidation [Malondialdehyde (MDA) content] were explored in leaves of the candidate resistant and susceptible parent (ML1720 and Kopergaon respectively) along with their recombinant inbred lines (RILs) with consistent AUDPC score. Three way ANOVA revealed presence of significant three way interaction A (time period) × B (non-inoculated/inoculated) × C (different genotypes) at p < 0.001. DMRT (t4 = 4.674 p = 0.01) analysis identified that the activities of CAT 48 h after inoculation (hai), POX 12 hai, SOD 24 hai, PAL 24 hai as well as MDA content 48 hai were the earliest and most illustrative traits distinguishing the compatible/susceptible and incompatible/resistance interactions. It also elaborated elevated antioxidant level along with PAL activity and lower MDA content during resistance reaction strengthening their vital role in combating against pathogen-induced oxidative stress in much better way than the susceptible ones. Principal component analysis identified PAL 24hai (PC1 proportion = 0.985, loading = 0.964) as most significant trait among other identified ones. Current experiment substantiated the findings that fluctuations in biochemical traits are associated with stress mitigation strategies and provided effective biochemical components characterizing resistance in mungbean against C. canescens infection to a certain extent proposing a cost effective disease resistance index, which can be better called as a biochemical markers in mungbean against CLS resistance.

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Acknowledgements

Author is thankful to ICAR, New Delhi for SRF fellowship and to her Ph.D. mentor Prof. Ramesh Chand, Department of Mycology and Plant pathology for providing the mungbean parental lines and the pathogenic strain of Cercospora canescens for the present research work.

Funding

Fundimg was provided by Indian Council of Agricultural Research (ICAR), India (Grant No. SRF).

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Authors and Affiliations

  1. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India

    Priyanka Choudhary & Ramesh Chand

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  1. Priyanka Choudhary
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  2. Ramesh Chand
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Correspondence to Priyanka Choudhary.

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Choudhary, P., Chand, R. Biochemical dynamics during plant pathogen interaction and its potential as disease screening index in Cercospora canescens–mung bean interaction. Indian Phytopathology 75, 395–403 (2022). https://doi.org/10.1007/s42360-021-00453-w

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