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Recent advances in chemically defined and tunable hydrogel platforms for organoid culture

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Abstract

Recent developments in organoid culture technologies have made it possible to closely recapitulate intrinsic characteristics of different tissues under in vitro conditions. These organoids act as a translational bridge between the traditional 2D/3D cultures and the in vivo models for studying the tissue development processes, disease modeling, and drug screening. Matrigel and tissue-specific extracellular matrix have been shown to support organoid development, efficiently; however, their chemically undefined nature, non-tunable properties, and associated batch-to-batch variations often limit reproducibility of the assembly process. In this regard, chemically defined platforms offer wider opportunities to optimize and recreate tissue-specific microenvironment. The present review delineates the current research trends in this sphere, focusing on material perspective and the target tissues (e.g., neural, liver, pancreatic, renal, and intestinal). The review winds up with a discussion on the current limitations and future perspective to provide a basis for future research.

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Fig. 1
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Fig. 3

Reproduced with permission from Ref. [94]. Copyright © 2016, Ranga et al.

Fig. 4

Reproduced with permission from Ref. [101]. Copyright © 2020, Sorrentino et al.

Fig. 5

Reproduced with permission from Ref. [75]. Copyright © 2020, Ye et al.

Fig. 6

Reproduced with permission from Ref. [56]. Copyright © 2020, Georgakopoulos et al.

Fig. 7

Reproduced with permission from Ref. [59]. Copyright © 2017, Elsevier B. V.

Fig. 8

Reproduced with permission from Ref. [74]. Copyright © 2020 Elsevier B. V.

Fig. 9

Reproduced with permission from Ref. [52]. Copyright © 2018, Broguiere et al. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

Fig. 10

Reproduced with permission from Ref. [74]. Copyright © 2020, Elsevier B. V.

Fig. 11

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Article 01 December 2022

Zixuan Zhao, Xinyi Chen, … Hanry Yu

Abbreviations

2D:

Two dimensional

3D:

Three dimensional

SD:

Standard deviation

ECM:

Extracellular matrix

EHS:

Engelbreth–Holm–Swarm

LMN:

Laminin

COLIV:

Collagen IV

HA:

Hyaluronic acid

PEG:

Polyethylene glycol

PIC:

Polyisocyanopeptide

MMPs:

Matrix metalloproteinases

GLDH:

Glutamate dehydrogenase

ESCs:

Embryonic stem cells

HSPCs:

Hematopoietic stem and progenitor cells

BMSCs:

Bone marrow stromal cells

PEGDA:

Poly(ethylene glycol) diacrylate

MSC:

Mesenchymal stem cells

FN:

Fibronectin

DV:

Dorsal–ventral

iPSCs:

Induced pluripotent stem cells

ALB:

Albumin

MDR1:

Multidrug resistance protein 1

CNF:

Cellulose nanofibril

ALAT:

Alanine aminotransferase

ASAT:

Aspartate transaminase

PEGDE:

Poly(ethylene glycol) diglycidyl ether

LGR5:

Leucine-rich repeat-containing G-protein-coupled receptor 5

BME2:

Basement Membrane Extract Type 2

dPMP:

Degradable PEG-MMP-PEG

ndPH:

Non-degradable PEG–heparin

dPMH:

Degradable PEG-MMP-Heparin

LEC:

LMN–entactin complex

HNF4α:

Hepatocyte nuclear factor 4α

E-cad:

E-cadherin

KRT19:

Cytokeratin 19

NHE3:

Sodium–hydrogen exchanger 3

Muc2:

Mucin 2

Lyz:

Lysozyme

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Acknowledgements

TA would like to acknowledge the INSPIRE scheme, Department of Science and Technology, Government of India, for providing the fellowship. Graphical abstract was created with BioRender.com. Authors would like to acknowledge the efforts of Ms. Sampriti Pal, Department of Biotechnology, Indian Institute of Technology Kharagpur, India, in proofreading the manuscript.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, 721302, India

    Tarun Agarwal & Tapas K. Maiti

  2. Institute of Physical Chemistry – Polish Academy of Sciences, Warsaw, Poland

    Nehar Celikkin & Marco Costantini

  3. Centre for Micro-BioRobotics, Istituto Italiano di Tecnologia, Viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy

    Pooyan Makvandi

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  1. Tarun Agarwal
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TA contributed to conceptualization, writing—original draft, and writing—reviewing and editing; NC and MC performed writing—original draft; TKM contributed to conceptualization and writing—reviewing and editing; PM helped in writing–reviewing and editing.

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Correspondence to Tapas K. Maiti.

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Agarwal, T., Celikkin, N., Costantini, M. et al. Recent advances in chemically defined and tunable hydrogel platforms for organoid culture. Bio-des. Manuf. 4, 641–674 (2021). https://doi.org/10.1007/s42242-021-00126-7

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