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Determination of the role of HrpN effector protein, as a key factor in course of interaction between Erwinia amylovora with chloroplasts of pear (Pyrus communis L.)

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Abstract

Fire blight caused by the gram-negative bacterium Erwinia amylovora is the most destructive disease of pome fruit trees. The pathogen induces the disease by secreting HrpN, HrpW, and DspA/E effector proteins into host tissues triggering oxidative burst. To study the role of these effectors in the course of interaction with host plants, hrpN−, hrpW− and dspA/E− mutants were inoculated on two pear cultivars under in situ (greenhouse and immature fruit) and in vitro systems. In addition, the determinantal role of these effectors on host chloroplasts was studied under activated and inactivated electron cascade of chloroplasts. Results showed that the lack of HrpN and DspA/E effectors postponed the initiation of necrosis and also decreased necrosis progress rate in comparison with the wild type strain. In contrast, hrpW− had the least decline in the pathogenicity of fire blight. The comparison of chloroplast activity confirmed the role of effectors in in situ experiments and also revealed that HrpN could be the main factor for the interaction of the pathogen with host cell chloroplasts.

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References

  • Abdollahi H, Ghahremani Z (2011) The role of chloroplasts in the Erwinia amylovora and the host plants. Acta Hortic 896:215–221

    Google Scholar 

  • Abdollahi H, Rugini E, Ruzzi M, Muleo R (2004) In vitro system for studying the interaction between Erwinia amylovora and genotypes of pear. Plant Cell Tiss Org 79:90–95

    Google Scholar 

  • Abdollahi H, Ghahremani Z, Erfani Nia K (2015) Role of electron transport chain of chloroplasts in oxidative burst of interaction between Erwinia amylovora and host cells. Photosynth Res 124:231–242

    CAS  Google Scholar 

  • Bellemann P, Geider K (1992) Localization of transposon insertions in pathogenicity mutants of Erwinia amylovora and their biochemical characterization. J Gen Microbiol 138:931–940

    CAS  Google Scholar 

  • Bhattacharjee S (2011) Sites of generation and physicochemical basis of formation of reactive oxygen species in plant cell in: DuttaGupta, S (Ed) reactive oxygen species and antioxidants in higher plants (pp 1-30) CRC press: Boca Raton

  • Boccara M, Schwartz W, Guiot E, Vidal G, De Paepe R, Dubois A, Boccara AC (2007) Early Chloroplastic alterations analyzed by optical coherence tomography during a harpin–induced hypersensitive response. Plant J 50:338–346

    CAS  Google Scholar 

  • Bogdanove AJ, Bauer DW, Beer SV (1998a) Erwinia amylovora secretes DspE, a pathogenicity factor and functional AvrE homolog, through the Hrp (type III secretion) pathway. J Bacteriol 180:2244–2247

    CAS  Google Scholar 

  • Bogdanove AJ, Kim JF, Wei ZM, Kolchinsky I, Charkowski AO, Conlin AK, Collmer A, Beer SV (1998b) Homology and functional similarity of a hrp–linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. PNAS USA 95:1325–1330

    CAS  Google Scholar 

  • Bonasera JM, Meng X, Owens T, Beer SV (2006) Interaction of DspE/a, a pathogenicity/avriulence protein of Erwinia amylovora, with pre–ferredoxin from apple and its relationship to photosynthetic efficiency. Acta Hortic 704:473–477

    CAS  Google Scholar 

  • Boureau T, El Maarouf-Boutea H, Garnier A, Brisset MN, Perino C, Pucheu I, Barny MA (2006) DspA/E, a type III effector essential for Erwinia amylovora pathogenicity and growth in planta, induces cell death in host apple and non–host tobacco plants. Mol Plant-Microbe Interact 12:312–329

    Google Scholar 

  • DebRoy S, Thilmony R, Kwack YB, Nomura K, He SY (2004) A family of conserved bacterial effectors inhibits salicylic acid–mediated basal immunity and promotes disease necrosis in plants. PNAS USA 101:9927–9932

    CAS  Google Scholar 

  • Degrave A, Fagard M, Perino S, Brisset MN, Gaubert S, Laroche S, Patrit O, Barny MA (2008) Erwinia amylovora type three–secreted proteins trigger cell death and defence responses in Arabidopsis thaliana. Mol Plant-Microbe Interact 21:1076–1086

    CAS  Google Scholar 

  • Dong H, Delaney T, Bauer D, Beer S (1999) Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene. Plant J 20:207–215

    CAS  Google Scholar 

  • Erfaninia K, Abdollahi H, Khosroshahi M (2014) Effect of several chloroplast electron transport chain inhibitors on interaction of Erwinia amylovora with susceptible and tolerant apple and pear cultivars. Plant Pathol 49:201–214 (In Farsi)

    Google Scholar 

  • Gaudriault S, Malandrin L, Paulin JP, Barny MA (1997) DspA, an essential pathogenicity factor of Erwinia amylovora showing homology with AvrE of Pseudomonas syringae, is secreted via Hrp secretion pathway in a DspB–dependent way. Mol Microbiol 26:1057–1069

    CAS  Google Scholar 

  • Khan MA, Zhao YF, Korban SS (2012) Molecular mechanisms of pathogenesis and resistance to the bacterial pathogen Erwinia amylovora, causal agent of fire blight disease in Rosaceae. Plant Mol Biol Rep 30:247–260

    CAS  Google Scholar 

  • Kim JF, Beer SV (1998) HrpW of Erwinia amylovora, a new harpin that contains a domain homologous to pectatelyases of a distinct class. J Bacteriol 180:5203–5210

    CAS  Google Scholar 

  • Menggad M, Laurent J (1998) Mutations in ams genes of Erwinia amylovora affect the interactions with host plants. Eur J Plant Pathol 104:313–322

    CAS  Google Scholar 

  • Oh CS, Beer SV (2005) Molecular genetics of Erwinia amylovora involved in the development of fire blight. FEMS Microbiol Lett 253:185–192

    CAS  Google Scholar 

  • Paret ML, deSilva AS, Criley RA, Alvarez AM (2008) Ralstonia solanacearum race 4: risk assessment for edible ginger floricultural ginger industries in Hawaii. Hort Technol 18:90–96

    Google Scholar 

  • Percival GC, Henderson A (2003) An assessment of the freezing tolerance of urban trees using chlorophyll fluorescence. J Hortic Sci Biotechnol 78:254–260

    Google Scholar 

  • Quoirin M, Lepoivre P (1977) Etude de milieux adaptes aux cultures in vitro de Prunus. Acta Hortic 78:437–442

    Google Scholar 

  • Salehi Z (2016) Evaluation of salicylic acid effects on tolerance to the biotic and abiotic stresses in apple and pears dissertation, Azad University of Karaj, Iran

  • Steinberger EM, Cheng GY, Beer SV (1990) Characterization of a 56–kb plasmid of Erwinia amylovora Ea322: its non–involvement in pathogenicity. Plasmid 24:12–24

    CAS  Google Scholar 

  • Venisse JS, Barny MA, Paulin JP, Brisset MN (2003) Involvement of three pathogenicity factors of Erwinia amylovora in the oxidative stress associated with compatible interaction in pear. FEBS Lett 537:198–202

    CAS  Google Scholar 

  • Venisse JS, Gullner G, Brisset MN (2001) Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora. Plant Physiol 125:2164–2172

    CAS  Google Scholar 

  • Wang D, Korban S, Pusey PL, Zhao Y (2012) AmyR is a novel negative regulator of Amylovoran production in Erwinia amylovora. PLoS One. https://doi.org/10.1371/journalpone0045038

  • Wei ZM, Beer SV (1996) Harpin from Erwinia amylovora induces plant resistance. Acta Hortic 411:223–226

    CAS  Google Scholar 

  • Wei ZM, Laby RJ, Zumoff CH, Bauer DW, He SY, Collmer A, Beer SV (1992) Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora. Science 257:85–88

    CAS  Google Scholar 

  • Xie Z, Chen Z (2000) Harpin induced hypersensitive cell death is associated with altered mitochondrial functions in tobacco cells. Mol Plant-Microbe Interact 13:183–190

    CAS  Google Scholar 

  • Yabuta Y, Mieda T, Rapolu M, Nakamura A, Motoki T, Maruta T, Yoshimura K, Ishikawa T, Shigeoka S (2007) Light regulation of ascorbate biosynthesis is dependent on the photosynthetic electron transport chain but independent of sugars in Arabidopsis. J Exp Bot 58:2661–2671

    CAS  Google Scholar 

  • Zhao YF, Qi M (2011) Comparative genomics of Erwinia amylovora and related Erwinia species–what do we learn? Genes 2:627–639

    CAS  Google Scholar 

  • Zhao YF, Blumer SE, Sundin GW (2005) Identification of Erwinia amylovora genes induced during infection of immature pear tissue. J Bacteriol 187:8088–8103

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

    Azam Taheri Shahrestani & Omid Eini Gandomani

  2. Temperate Fruits Research Centre, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

    Hamid Abdollahi

  3. Industrial and Environmental Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

    Bagher Yakhchali

  4. Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

    Rahim Mehrabi

Authors
  1. Azam Taheri Shahrestani
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  2. Hamid Abdollahi
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  3. Bagher Yakhchali
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  4. Rahim Mehrabi
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  5. Omid Eini Gandomani
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Correspondence to Azam Taheri Shahrestani.

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All the authors certify that 1) do not have any actual or potential conflict of interest, 2) the study described is original and has not been published previously, and is not under consideration for publication elsewhere, 3) all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected.

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Taheri Shahrestani, A., Abdollahi, H., Yakhchali, B. et al. Determination of the role of HrpN effector protein, as a key factor in course of interaction between Erwinia amylovora with chloroplasts of pear (Pyrus communis L.). J Plant Pathol 102, 1041–1050 (2020). https://doi.org/10.1007/s42161-020-00640-0

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  • DOI: https://doi.org/10.1007/s42161-020-00640-0

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