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Occurrence of plant viruses on pepper cultivated in open fields in R. Macedonia and partial characterization of cucumber mosaic virus isolates

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Abstract

Screening of pepper plants cultivated in open fields for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV) and potato virus Y (PVY) in the Republic of Macedonia was performed by DAS-ELISA from 2012 to 2014. During the tested period, the predominant infection was by CMV, ranging from 34 to 61% of the tested samples, followed in 2012 by AMV and in 2013 and 2014 by PVY. Molecular detection and identification of CMV on selected pepper leaf samples was done by Reverse Transcription – Polymerase Chain Reaction (RT-PCR) and sequencing of the coat protein (CP) gene. Genetic relationships of the 18 CMV isolates generated in this study were compared with isolates from other parts of the world. Phylogenetic analysis was conducted, based on the partial sequences of the CP gene. In the constructed phylogenetic tree, all of the 18 Macedonian CMV isolates were clustered in the IA subgroup.

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Acknowledgements

We thank the Department of Phytopathology from the Faculty of Agriculture in the University of Belgrade, Serbia, especially Prof. Branka Krstić, Prof. Ivana Stanković, Dr. Katarina Milojević and Dr. Ana Vučurović for their know-how in processing of the nucleotide sequences of the CMV isolates.

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Authors and Affiliations

  1. Department of Plant Protection, Institute of Agriculture, University “Ss. Cyril and Methodius”, blvd.”16-ta Makedonska Brigada” no.3A, 1000, Skopje, Macedonia

    Katerina Bandjo Oreshkovikj

  2. Department of Phytopathology, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius”, blvd.”Aleksandar Makedonski” bb, 1000, Skopje, Macedonia

    Rade Rusevski & Biljana Kuzmanovska

  3. Department of Genetics and Plant Breeding, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius”, blvd.”Aleksandar Makedonski” bb, 1000, Skopje, Macedonia

    Mirjana Jankulovska

  4. Department of Biochemistry and Genetic Engineering, Faculty of Agricultural Sciences and Food, University “Ss. Cyril and Methodius”, blvd.”Aleksandar Makedonski” bb, 1000, Skopje, Macedonia

    Zoran T. Popovski

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  1. Katerina Bandjo Oreshkovikj
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  2. Rade Rusevski
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  3. Biljana Kuzmanovska
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  4. Mirjana Jankulovska
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  5. Zoran T. Popovski
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Correspondence to Katerina Bandjo Oreshkovikj.

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Oreshkovikj, K.B., Rusevski, R., Kuzmanovska, B. et al. Occurrence of plant viruses on pepper cultivated in open fields in R. Macedonia and partial characterization of cucumber mosaic virus isolates. J Plant Pathol 100, 485–491 (2018). https://doi.org/10.1007/s42161-018-0110-2

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  • DOI: https://doi.org/10.1007/s42161-018-0110-2

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