Abstract
Leaves of Welsh onion (Allium fistulosum) showing blight and elongated lesions were observed and sampled in central west Taiwan in 2016, from which a bacterium phenotypically similar to Pantoea spp. was isolated. The results of molecular identification based on 16S rDNA and gyrB sequences indicate that the Welsh onion isolates are Pantoea ananatis. Upon artificial inoculation of Welsh onion leaves, P. ananatis isolates induced symptoms indistinguishable from those observed on the naturally infected plants. The bacterium was consistently re-isolated from inoculated leaves, fulfilling Koch’s postulates. To the best of our knowledge, this is the first report of P. ananatis causing leaf blight of Welsh onion in Taiwan. The Welsh onion-pathogenic P. ananatis isolates showed copper resistance, implying that copper-based bactericides may not provide effective protection of Welsh onion against P. ananatis.
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References
Behlau F, Hong JC, Jones JB, Graham JH (2013) Evidence for acquisition of copper resistance genes from different sources in citrus-associated xanthomonads. Phytopathology 103:409–418
Brady C, Cleenwerck I, Venter S, Vancanneyt M, Swings J, Coutinho T (2008) Phylogeny and identification of Pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (MLSA). Syst Appl Microbiol 31:447–460
Bruton BD, Wells JM, Lester GEP, Patterson CL (1991) Pathogenicity and characterization of Erwinia ananas causing a postharvest disease of cantaloup fruit. Plant Dis 75:180–183
Ceponis MJ, Wells JM, Cappellini RA (1985) Bacterial brown spot of honeydew melons. Hortscience 20:302–303
Constantin EC, Cleenwerck I, Maes M, Baeyen S, Van Malderghem C, De Vos P, Cottyn B (2016) Genetic characterization of strains named as Xanthomonas axonopodis pv. dieffenbachiae leads to a taxonomic revision of the X. axonopodis species complex. Plant Pathol 65:792–806
Cother EJ, Reinke R, McKenzie C, Lanoiselet VM, Noble DH (2004) An unusual stem necrosis of rice caused by Pantoea ananas and the first record of this pathogen on rice in Australia. Australas Plant Pathol 33:495–503
Coutinho T, Venter SN (2009) Pantoea ananatis: an unconventional plant pathogen. Mol Plant Pathol 10:325–335
Dutta B, Barman AK, Srinivasan R, Avci U, Ullman DE, Langston DB, Gitaitis RD (2014) Transmission of Pantoea ananatis and P. agglomerans, causal agents of center rot of onion (Allium cepa), by onion thrips (Thrips tabaci) through feces. Phytopathology 104:812–819
Gent D., H., Al-Saadi A., Gabriel D.W., Louws F.J., Ishimaru C.A., Schwartz H.F., 2005. Pathogenic and genetic relatedness among Xanthomonas axonopodis pv. allii and other pathovars of X. axonopodis. Phytopathology 95: 918–925
Gitaitis RD, Gay JD (1997) First report of a leaf blight, seed stalk rot, and bulb decay of onion by Pantoea ananas in Georgia. Plant Dis 81:1096
Gitaitis RD, Walcott RR, Wells ML, Diaz Perez JC, Sanders FH (2003) Transmission of Pantoea ananatis, causal agent of center rot of onion, by tobacco thrips, Frankliniella fusca. Plant Dis 87:675–678
Green M.R., Sambrook J., 2012. Molecular cloning: a laboratory manual, 4 ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, NY, USA
Hattingh MJ, Walters DF (1981) Stalk and leaf necrosis of onion caused by Erwinia herbicola. Plant Dis 65:615–618
Hsu ST (2002) List of plant diseases in Taiwan. The Phytopathological Society of the Republic of China. Taichung, Taiwan
Huang Z, Du CC (1960) Studies on Erwinia ananas fruitlet brown rot disease of pineapple. Plant Prot Bull 2:125–126
Kadota I, Uehara K, Shinohara H, Nishiyama K (2000) Bacterial blight of welsh onion: a new disease caused by Xanthomonas campestris pv. allii pv. Nov. J Gen Plant Pathol 66:310–315
Kido K, Hasegawa M, Matsumoto H, Kobayashi M, Takikawa Y (2010) Pantoea ananatis strains are differentiated into three groups based on reactions of tobacco and welsh onion and on genetic characteristics. J Gen Plant Pathol 76:208–218
Lu FM, Lee HS (1985) The infestation and chemical control of onion thrips, Thrips tabaci Linderman. (Thysanoptera: Thripidae). J Agric Res China 34:223–227
Schaad N.W., Jones J.B., Chun W., 2001. Laboratory guide for identification of plant pathogenic Bacteria, 3 ed. APS Press, St. Paul, MN, USA
Serrano FB (1928) Bacterial fruitlet brown-rot of pineapple in the Philippine. Philipp J Sci 36:271–305
Tabei H, Azegami K, Fukuda T (1988) Infection site of rice grain with Erwinia herbicola, the causal agent of bacterial Palea browning of rice. Ann Phytopathol Soc Jpn 54:637–639
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
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This work was financed by the Ministry of Science and Technology, Taiwan, R.O.C. (grant number 104-2313-B-415-013-MY2).
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C.J.H. conceived this work. C.J.H. and Y.J.W. designed and carried out most of the experiments. C.J.H analyzed the data and wrote the manuscript. C.H.L. was involved in data analysis and revised the manuscript critically. All authors have approved the final version of manuscript.
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Wang, YJ., Lin, CH. & Huang, CJ. Occurrence, identification, and bactericide sensitivity of Pantoea ananatis causing leaf blight on welsh onion in Taiwan. J Plant Pathol 100, 309–312 (2018). https://doi.org/10.1007/s42161-018-0067-1
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DOI: https://doi.org/10.1007/s42161-018-0067-1