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Optical Biosensing of Bacteria and Bacterial Communities

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Abstract

Bacterial sensing is important for understanding the numerous roles bacteria play in nature and in technology, understanding and managing bacterial populations, detecting pathogenic bacterial infections, and preventing the outbreak of illness. Current analytical challenges in bacterial sensing center on the dilemma of rapidly acquiring quantitative information about bacteria with high detection efficiency, sensitivity, and specificity, while operating within a reasonable budget and optimizing the use of ancillary tools, such as multivariate statistics. This review starts from a general description of bacterial sensing methods and challenges, and then focuses on bacterial characterization using optical methods including Raman spectroscopy and imaging, infrared spectroscopy, fluorescence spectroscopy and imaging, and plasmonics, including both extended and localized surface plasmon resonance spectroscopy. The advantages and drawbacks of each method in relation to the others are discussed, as are their applications. A particularly promising direction in bacterial sensing lies in combining multiple approaches to achieve multiplex analysis, and examples where this has been achieved are highlighted.

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Acknowledgement

Work in the authors’ laboratory described in this article was funded by the National Institute of Allergies and Infectious Diseases under Grant 1RO1AI113219-01.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, 46556, USA

    Jiayun Hu & Paul W. Bohn

  2. Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, 46556, USA

    Paul W. Bohn

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  1. Jiayun Hu
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  2. Paul W. Bohn
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Correspondence to Paul W. Bohn.

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Hu, J., Bohn, P.W. Optical Biosensing of Bacteria and Bacterial Communities. J. Anal. Test. 1, 4 (2017). https://doi.org/10.1007/s41664-017-0002-z

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  • DOI: https://doi.org/10.1007/s41664-017-0002-z

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