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A high-throughput screening method for breeding Aspergillus niger with 12C6+ ion beam-improved cellulase

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Abstract

In this study, a high-throughput screening method was established through the 24-square deep-well microliter plate (MTP) fermentation and micro-plate detection for large-scale screening of the mutants. It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation. Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation. The filter paper activity and β-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23 % separately compared with A. niger H11 by shaking flask fermentation, and it was genetically stable after being passaged to nine generations. The results indicate that the high-throughput screening method can be used for the quick breeding of A. niger with high cellulase activity.

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Acknowledgements

We sincerely thank the National Laboratory of HIRFL for giving us opportunity to perform this project.

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Authors and Affiliations

  1. Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd, Lanzhou, 730000, China

    Bo-Ling Jiang, Shu-Yang Wang, Yu-Chen Wang, Ji-Hong Chen, Wen-Jian Li, Jing Liu, Wei Hu, Guo-Qing Xiao, Miao-Ying Dong & Fu-Qiang Xu

  2. University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing, 100049, China

    Bo-Ling Jiang

  3. Lanzhou University, 222 South Tianshui Road, Lanzhou, 730000, Gansu Province, China

    Shu-Yang Wang

  4. Gansu Agricultural University, Lanzhou, 730000, Gansu Province, China

    Yu-Chen Wang

Authors
  1. Bo-Ling Jiang
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  2. Shu-Yang Wang
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  3. Yu-Chen Wang
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  4. Ji-Hong Chen
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  7. Wei Hu
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Corresponding author

Correspondence to Shu-Yang Wang.

Additional information

This work was supported by the National Natural Science Foundation of China (No. 11305225), the Science and Technology Service Network Initiative (KFJ-EW-STS-086).

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Jiang, BL., Wang, SY., Wang, YC. et al. A high-throughput screening method for breeding Aspergillus niger with 12C6+ ion beam-improved cellulase. NUCL SCI TECH 28, 1 (2017). https://doi.org/10.1007/s41365-016-0157-8

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  • DOI: https://doi.org/10.1007/s41365-016-0157-8

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