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Mating types of Phomopsis viticola isolates from grapevine in Aegean Region/Turkey

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Abstract

Mating type diversity has been recently studied in Diaporthe/Phomopsis isolates from different hosts and geographical origin. Two idiomorphic mating genes, MAT1-1-1 and MAT1-2-1, each related to different mating types, have been used for developing PCR markers for mating type determination. The limited number of Phomopsis viticola isolates from grapevine has been identified as heterothallic, carrying one of these mating genes. Mating types of P. viticola have not been studied in a broad sense at the population level, in any countries, including Turkey. The objective of this study is to determine mating types of P. viticola isolates collected from the major grape-producing areas of the Aegean Region. A total of 150 P. viticola isolates obtained from grapevine shoots were subjected to PCR assay with the two pairs of specific primers for mating type determination. All of the isolates produced a single amplicon approximately 229 bp in size, which is known to belong to the MAT1-2-1 gene. No amplicon product of mating type 1 was found. With this background, sexual reproduction of P. viticola is unlikely in our area of study. It can be assumed that P. viticola reproduces asexually and its population is mainly clonal in western Turkey.

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Acknowledgements

We would like to thank the Agricultural Biotechnology and Food Safety Application and Research Center Aydin Adnan Menderes University Aydin-Turkey (ADÜ-TARBİYOMER), for providing us the opportunity for DNA Extraction analysis of this study.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, 9100, Aydin, Turkey

    Sahra Hosseinalizadeh, Ömer Erincik & Serap Açıkgöz

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  1. Sahra Hosseinalizadeh
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  2. Ömer Erincik
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  3. Serap Açıkgöz
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Correspondence to Sahra Hosseinalizadeh.

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Hosseinalizadeh, S., Erincik, Ö. & Açıkgöz, S. Mating types of Phomopsis viticola isolates from grapevine in Aegean Region/Turkey. J Plant Dis Prot 128, 1269–1273 (2021). https://doi.org/10.1007/s41348-021-00492-5

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