Abstract
Postharvest anthracnose disease caused by Colletotrichum species is one of the main threats to banana production because it reduces the quality, the marketability, and the consumption of the fruit. From June to September 2017, coalescent sunken necrotic lesions were observed on the banana fruit cv. Tabasco (Musa acuminata), harvested in two orchards in Teapa, Tabasco, southeast of Mexico. Thus, this research aimed to identify the causal agents of necrotic lesions that affect banana production. Isolates of Colletotrichum spp. obtained from the sunken necrotic lesions were studied by morphological and multilocus phylogenetic approaches. Amplification and sequencing of the six (GAPDH, CHS-1, ACT, TUB, GS, and APMAT) partial genes were performed. Later, individual alignment for each gene was created and concatenated. Bayesian inference and maximum likelihood analyses revealed that the three representative strains belong to C. chrysophilum, a member of C. gloeosporioides species complex. To determine whether C. chrysophilum strains were responsible for the symptoms on banana fruit, a pathogenicity test was conducted by inoculation of wounded fruit. Typical necrotic lesions were observed 8 days after inoculation, while the control fruit remained healthy. This finding represents the first report of C. chrysophilum causing anthracnose of banana in Mexico; therefore, it should be considered an integrated management program to reduce losses caused by this disease.
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References
Bellaire LL, Chillet M, Mourichon X (2007) Elaboration of an early quantification method of quiescent infections of Colletotrichum musae on bananas. Plant Dis 84:128–133
Cai L, Hyde KD, Taylor PWJ, Weir BS, Waller JM, Abang MM, Zhang JZ, Yang YL, Phoulivong S, Liu ZY, Prihastuti H, Shivas RG, McKenzie EHC, Johnston PR (2009) A polyphasic approach for studying Colletotrichum. Fungal Divers 39:183–204
Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556
Damm U, Sato T, Alizadeh A, Groenewald JZ, Crous PW (2019) The Colletotrichum dracaenophilum, C. magnum and C. orchidearum species complexes. Stud Mycol 92:1–46
Damm U, Cannon PF, Woudenberg JHC, Crous PW (2012) The Colletotrichum acutatum species complex. Stud Mycol 73:37–113
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Food and Agriculture Organization of the United Nations (2018) FAOSTAT statistical database. https://www.fao.org/faostat/en/#data/QC. Accessed 5 Apr 2020
Fuentes-Aragón D, Juárez-Vázquez SB, Vargas-Hernández M, Silva-Rojas HV (2018) Colletotrichum fructicola, a member of Colletotrichum gloeosporioides sensu lato, is the causal agent of anthracnose and soft rot in avocado cv. Hass. Mycobiology 46:92–100
Fuentes-Aragón D, Guarnaccia V, Rebollar-Alviter A, Juárez-Vázquez SB, Aguirre-Rayo F, Silva-Rojas HV (2020) Multilocus identification and thiophanate methyl sensitivity of Colletotrichum gloeosporioides species complex associated with fruit with symptoms and symptomless leaves of mango. Plant Pathol 69:1125–1138
Glass NL, Donaldson G (1995) Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes. App Environ Microbiol 61:1323–1330
Guerber JC, Liu B, Correll JC, Johnston PR (2003) Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95:872–895
Intan MSA, Suzianti IV, Latiffah Z (2013) First report of Colletotrichum gloeosporioides causing anthracnose of banana (Musa spp.) in Malaysia. Plant Dis 9(7):991
Juárez-Vázquez SB, Silva-Rojas HV, Rebollar-Alviter A, Maidana-Ojeda M, Osnaya-González M, Fuentes-Aragón D (2019) Phylogenetic and morphological identification of Colletotrichum godetiae, a novel pathogen causing anthracnose on loquat fruit (Eriobotrya japonica). J Plant Dis Prot 126:593–598
Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on Fourier transform. Nucleic Acids Res 30:3059–3066
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analyses of sequence data. Bioinformatics 28:1647–1649
Lim J, Lim TH, Cha B (2002) Isolation and identification of Colletotrichum musae from imported bananas. Plant Pathol J 18:161–164
Liu F, Cai L, Crous PW, Damm U (2013) Circumscription of the anthracnose pathogens Colletotrichum lindemuthianum and C. nigrum. Mycologia 105:844–860
Liu F, Cai L, Crous PW, Damm U (2014) The Colletotrichum gigasporum species complex. Persoonia 33:83–97
Maddison WP, Maddison DR (2018) Mesquite: a modular system for evolutionary analyses version 3.51 [Internet]. https://mesquiteproject.org
O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Mol Phylogenet Evol 7:103–116
Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256
Riera N, Ramirez-Villacis D, Barriga-Medina N, Alvarez-Santana J, Herrera K, Ruales C, Leon-Reyes A (2019) First report of banana anthracnose caused by Colletotrichum gloeosporioides in Ecuador. Plant Dis 103:763
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542
Sharma G, Pinnaka AK, Shenoy BD (2015) Resolving the Colletotrichum siamense species complex using ApMat marker. Fungal Divers 71:247–264
Silva DN, Talhinhas P, Várzea V, Cai L, Paulo OS, Batista D (2012) Application of the Apn2/MAT locus to improve the systematics of the Colletotrichum gloeosporioides complex: an example from coffee (Coffea spp.) hosts. Mycologia 104:396–409
Silvestro D, Michalak I (2012) raxmlGUI: a graphical front-end for RAxML. Org Divers Evol 12:335–337
Su YY, Noireung P, Liu F, Hyde KD, Moslem MA, Bahkali AH, Abd-Elsalam KA, Cai L (2011) Epitypification of Colletotrichum musae, the causative agent of banana anthracnose. Mycoscience 52:376–382
Veloso JS, Câmara MPS, Lima WG, Michereff SJ, Doyle VP (2018) Why species delimitation matters for fungal ecology: Colletotrichum diversity on wild and cultivated cashew in Brazil. Fungal Biol 122:677–691
Vieira WAS, Lima WG, Nascimento ES, Michereff SJ, Câmara MPS, Doyle VP (2017) The impact of phenotypic and molecular data on the inference of Colletotrichum diversity associated with Musa. Mycologia 109:912–934
Zhou Y, Huang JS, Yang LY, Wang GF, Li JQ (2017) First report of banana anthracnose caused by Colletotrichum scovillei in China. Plant Dis 101:381–382
Funding
The research was partially financed by the Laboratory of Seed Biotechnology and Plant Pathology at the Postgraduate College, Mexico, through project PS 19–4007.
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Fuentes-Aragón, D., Rebollar-Alviter, A., Osnaya-González, M. et al. Multilocus phylogenetic analyses suggest the presence of Colletotrichum chrysophilum causing banana anthracnose in Mexico. J Plant Dis Prot 128, 589–595 (2021). https://doi.org/10.1007/s41348-020-00396-w
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DOI: https://doi.org/10.1007/s41348-020-00396-w