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Comparison of indigenous Trichoderma spp. strains to a foreign commercial strain in terms of biocontrol efficacy against Colletotrichum nymphaeae and related biological features

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Abstract

Colletotrichum nymphaeae is the principal causal agent of strawberry anthracnose worldwide, including Iran. For disease management, eco-friendly alternatives such as biological control instead of chemical fungicides are highly desirable. The aim of this study was to compare the efficacy of 20 strains of Trichoderma spp. indigenous to Iran against C. nymphaeae, to a commercial strain of Trichoderma atroviride (SC1) isolated elsewhere, under in vitro and in planta conditions. The tested strains belonged to T. harzianum (ten isolates), T. pleuroticola (five isolates), T. virens (three isolates), T. asperellum (one isolate) and T. afroharzianum (one isolate). In vitro results showed that most isolates used in this study were able to significantly inhibit mycelial growth of the pathogen in dual culture and non-volatile tests. The majority of the tested Trichoderma spp. isolates produced lytic enzymes, including chitinase, β-1,3-glucanase, protease and cellulase. Besides producing hydrogen cyanide, Trichoderma spp. strains were also shown to be positive in terms of siderophore production and the mycoparasitism process. In planta bioassays revealed that the best indigenous strain (T. virens CCTUT4) significantly reduced anthracnose, at the same rate as the commercial strain, although the standard chemical fungicide (Switch®) was superior in terms of disease control. A positive correlation between disease incidence and severity reduction was detected with increasing population density of the antagonists in this study. The findings of this study indicate that isolating Trichoderma-based biocontrol agents from the crop against whose disease are targeted does not necessarily provide advantages in comparison with a strain having good biocontrol properties and originating from a different substrate.

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Acknowledgements

The authors would like to thank the Research Deputy of the University of Tabriz and the EU INNOVA project (FP7-People-2012-IAPP, Grant Agreement 324416).

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Authors and Affiliations

  1. Department of Plant Protection, University of Tabriz, P. O. Box 5166614766, Tabriz, Iran

    Kaivan Karimi, Asadollah Babai Ahari & Mahdi Arzanlou

  2. Department of Plant Protection, University of Kurdistan, Sanandaj, Iran

    Jahanshir Amini

  3. Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all’Adige, Italy

    Kaivan Karimi & Ilaria Pertot

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Karimi, K., Babai Ahari, A., Arzanlou, M. et al. Comparison of indigenous Trichoderma spp. strains to a foreign commercial strain in terms of biocontrol efficacy against Colletotrichum nymphaeae and related biological features. J Plant Dis Prot 124, 453–466 (2017). https://doi.org/10.1007/s41348-017-0088-6

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