Abstract
Sixty five grapevines in productive areas from southern regions in Poland were tested for the presence of Grapevine Pinot gris virus (GPGV) by RT-PCR. Twenty two percent of the tested plants were infected by GPGV. GPGV positive grapevines were also tested for the presence of other viruses by multiplex and simplex RT-PCR. Part of the movement and coat protein coding regions and a part of RdRp domain were sequenced. Phylogenetic analyses of the 2 genes revealed that south Polish GPGV isolates grouped with already described asymptomatic isolates. This study provides the first survey of the GPGV occurrence in Poland.
Abbreviations
- RdRp:
-
RNA-dependent RNA polymerase
- RT-PCR:
-
Reverse transcription polymerase chain reaction
- MP:
-
Movement protein
- CP:
-
Coat protein
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Acknowledgements
This work was supported by Projects Nos. LD15163 and LD14051 from the Ministry of Education, Youth and Sports, Czech Republic covered by COST Action FA1407 and FA1303. Access to computing and storage facilities owned by parties and projects contributing to the National Grid Infrastructure MetaCentrum, provided under the “Projects of Large Infrastructure for Research, Development and Innovations” programme (LM2010005), were greatly appreciated. The authors would like to thank Dr. Ivo Ondrášek from Mendel University in Brno for the establishment of valuable collaborations in Poland.
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Eichmeier, A., Pieczonka, K., Peňázová, E. et al. Occurrence of Grapevine Pinot gris virus in Poland and description of asymptomatic exhibitions in grapevines. J Plant Dis Prot 124, 407–411 (2017). https://doi.org/10.1007/s41348-017-0076-x
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DOI: https://doi.org/10.1007/s41348-017-0076-x