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The pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, and its pathogenicity to German Pinus sylvestris provenances

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Abstract

The pine wood nematode, Bursaphelenchus xylophilus, can cause pine wilt disease on susceptible conifer species, mainly Pinus spp., outside its natural range. One of the management options for pine wilt disease in recent decades in affected countries such as Japan was the evaluation of potentially tolerant or resistant host species. In the framework of Pest Risk Assessment and Management as well in Contingency Planning in Germany, we studied the pathogenicity of B. xylophilus towards different German pine provenances. According to the German Legal Ordinance on Regions of Provenance, 2- to 3-year-old Pinus sylvestris saplings of eight provenances were artificially inoculated with B. xylophilus using a suspension of 4000 B. xylophilus in 300 µl of tap water per tree. No significant differences in tree death between the provenances were detected. All inoculated provenances reached a mortality of 100 %, but significant differences occurred in the time course of disease development.

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Acknowledgments

This work was conducted at the Julius Kühn-Institut (JKI) in Brunswick in the framework of the EU-funded research project REPHRAME “Development of improved methods for detection, control and eradication of pine wood nematode in support of EU Plant Health Policy” [KBBE.2010.1.4-09]. Many thanks are owed to Doreen Mybes and Silvia Urban for their excellent laboratory help. We are also grateful to Dr. Irina Kuzyakova (Department Ecoinformatics, Biometrics and Forest Growth, Büsgen-Institute, Georg-August-University Göttingen) and Dr. Doreen Gabriel (Institute for Crop and Soil Science, JKI Brunswick) for advice with the statistical analysis. We thank Dr. Edmundo de Sousa (I.N.I.A, Portugal) for providing the nematode isolate. Apl. Prof. Dr. Johannes Hallmann (Institute for Epidemiology and Pathogen Diagnostics, JKI Münster) and an anonymous reviewer deserve many thanks for improving the manuscript.

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Authors and Affiliations

  1. Institute for National and International Plant Health, Federal Research Centre for Cultivated Plants, Julius Kühn-Institut, Messeweg 11/12, 38104, Brunswick, Germany

    Andrea Hopf-Biziks & Thomas Schröder

  2. Department of Forest Zoology and Forest Conservation, Büsgen-Institute, Büsgenweg 3, 37077, Göttingen, Germany

    Stefan Schütz

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  1. Andrea Hopf-Biziks
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  2. Thomas Schröder
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Correspondence to Andrea Hopf-Biziks.

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Andrea Hopf-Biziks, Thomas Schröder and Stefan Schütz declare that they have no conflict of interest.

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Hopf-Biziks, A., Schröder, T. & Schütz, S. The pine wood nematode, Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, and its pathogenicity to German Pinus sylvestris provenances. J Plant Dis Prot 123, 43–49 (2016). https://doi.org/10.1007/s41348-016-0005-4

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  • DOI: https://doi.org/10.1007/s41348-016-0005-4

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