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Characterization of Iranian Tomato aspermy virus isolates with a variant 2b gene sequence

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Abstract

Three isolates of Tomato aspermy virus (TAV) were identified from two ornamental plants, Petunia hybrida and Chrysanthemum morifolium, sampled in Kerman province in the southeastern region of Iran. The complete genome of one isolate (Ker.Mah.P) and three open reading frames (ORFs), encoding coat protein (CP), movement protein (MP) and 2b protein of the three isolates were sequenced. The three genome segments of the Ker.Mah.P isolate (RNA-1, −2 and −3) are comprised of 3351, 3035 and 2194 nts, respectively. The nucleotide sequences of the three Iranian isolates were compared with other available sequences in GenBank, and the genome of Ker.Mah.P showed >96% sequence identity with sequences of TAV isolates. In a host range study, the three Iranian isolates induced chlorotic and necrotic symptoms on the true leaves of Cucumis sativus cv. Maximum F4 and systemic mottle and necrotic local lesions on Chenopodium amaranticolor, whereas other isolates whose sequences are deposited in GenBank cause only necrotic local lesions on these test plants. Sequences of the 2b gene of the three Iranian and one Chinese isolate have an additional 27 nt insert and it is possible that this domain, which adds nine amino acids to the 2b protein, may affect the biological properties of the virus. To the best of our knowledge, this is the first report of a TAV isolate from the Middle East whose genome has been sequenced, as well as the first global report of the natural occurrence of TAV in P. hybrida.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

    Mohammad Maddahian, Hossein Massumi, Jahangir Heydarnejad & Akbar Hosseini Pour

  2. Young Researchers Society, Shahid Bahonar University of Kerman, Kerman, Iran

    Mohammad Maddahian

  3. Research and Technology Institute of Plant Production, Shahid Bahonar University of Kerman, Kerman, Iran

    Hossein Massumi, Jahangir Heydarnejad & Akbar Hosseini Pour

  4. The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, 85287-5001, USA

    Arvind Varsani

  5. Structural Biology Research Unit, University of Cape Town, Cape Town, 7701, South Africa

    Arvind Varsani

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  1. Mohammad Maddahian
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  2. Hossein Massumi
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  3. Jahangir Heydarnejad
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  4. Akbar Hosseini Pour
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Correspondence to Hossein Massumi.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Funding

This work was supported by a grant from Shahid Bahonar University of Kerman, Kerman, Iran. (Grant number 513614).

Additional information

Section Editor: Michael Goodin

The sequences reported in this manuscript have been deposited in GenBank, with access numbers KT757536–38, JQ403612–14, KT757534–35 and KT757539–40.

Electronic supplementary material

Supplementary Table S1

Position and schematics illustrating the primer pairs used to the amplify regions of the Ker.Mah.P isolate RNA-1, RNA-2 and RNA-3. (DOCX 16.1 kb)

Supplementary Fig. S1

(PPT 220 kb)

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Maddahian, M., Massumi, H., Heydarnejad, J. et al. Characterization of Iranian Tomato aspermy virus isolates with a variant 2b gene sequence. Trop. plant pathol. 42, 475–484 (2017). https://doi.org/10.1007/s40858-017-0173-1

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  • DOI: https://doi.org/10.1007/s40858-017-0173-1

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