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Assessing sequence diversity of Groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana

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Abstract

The incidence of Groundnut rosette assistor virus (GRAV) in farmers’ fields and sequence diversity of groundnut rosette disease (GRD) agents were assessed in the three northern groundnut production regions of Ghana. GRAV incidence was high (69.5 to 75.0%) but not significantly different between the regions. Nucleotide sequencing of GRAV coat protein (CP) gene revealed 99–100% identity among the Ghanaian isolates and 97–100% similarity to GRAV sequences from Nigeria and Malawi for both nucleotide and predicted amino acids. Nucleotide sequence identities of partial ORF3 and 4 of Groundnut rosette virus (GRV) among the Ghanaian isolates were more variable (89–100%). Ghanaian GRV isolates were more closely related in nucleotide sequence identity to Nigerian isolates (95–98%) than Malawian isolates (88–90%). Similarly, nucleotide identity within Ghanaian GRV-sat RNA’s were close (94–100%), but distinct from Nigerian (82–87%) and Malawian (82–86%) GRV-sat RNAs. Ghanaian isolates of all three agents of GRD showed no obvious isolate diversity patterns based on the regions from where they were collected. We present the first report on the distribution and genetic diversity of GRD agents in Ghana.

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Acknowledgements

We are grateful to the Tasmanian Institute of Agriculture and Biotechnology and Nuclear Agriculture Research Institute of the Ghana Atomic Energy Commission for the use of their facilities.

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Authors and Affiliations

  1. Tasmanian Institute of Agriculture, University of Tasmania, 13 St. Johns Avenue, New Town, TAS, 7008, Australia

    Andrew S. Appiah, Robert S. Tegg & Calum R. Wilson

  2. Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P.O. Box LG 80, Legon, Accra, Ghana

    Andrew S. Appiah

  3. College of Agronomy, Jilin Agricultural University, Changchun, 130118, Jilin Province, People’s Republic of China

    Frederick L. Sossah

  4. Biotechnology Centre, College of Basic and Applied Sciences, University of Ghana, Legon, Accra, Ghana

    Samuel K. Offei

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  1. Andrew S. Appiah
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  2. Frederick L. Sossah
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  3. Robert S. Tegg
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  4. Samuel K. Offei
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  5. Calum R. Wilson
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Correspondence to Calum R. Wilson.

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The study was supported by a scholarship grant from Australian Aid for International Development for African Development to Andrew S. Appiah.

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All authors declare they have no conflicts of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Section Editor: Michael Goodin

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Appiah, A.S., Sossah, F.L., Tegg, R.S. et al. Assessing sequence diversity of Groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana. Trop. plant pathol. 42, 109–120 (2017). https://doi.org/10.1007/s40858-017-0140-x

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  • DOI: https://doi.org/10.1007/s40858-017-0140-x

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