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Citrus leaf blotch virus in Cuba: first report and partial molecular characterization

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Abstract

Citrus leaf blotch virus (CLBV) infection has been detected in citrus cultivars from several countries including the USA, Spain, Italy, Japan and New Zealand. To investigate whether CLBV is present in Cuba, a survey was conducted in seven commercial citrus fields of the country during 2007–2013. In all, 1940 trees were inspected and samples of two symptomatic and 18 asymptomatic sources were tested by biological indexing and molecular techniques for virus detection. Bark patches from the two symptomatic Nagami and Round kumquat sources induced chlorotic blotching and vein clearing in Dweet tangor, and only for the Round kumquat inoculums, stem pitting in Etrog citron. RT-PCR assays using specific primers to amplify fragments of polymerase (RdRp), movement (MP) and coat protein (CP) genes of CLBV yielded amplicons with the expected sizes using RNA from both sources as a template. Viral RNA was further detected by a non-radioactive dot-blot hybridization assay using a mixture of specific probes derived from a Spanish isolate of CLBV. Sequences from RdRp, MP, and CP amplicons showed identical nucleotide identities for the two Cuban isolates, and higher than 95.9 % with isolates from Spain, New Zealand and the USA.

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Acknowledgments

This research was supported by the Grupo Empresarial Frutícola de Cuba, GEF, through the national projects: 566, 0571, 2004 and by the Cuban Ministry for Agriculture, project P131LH003001.

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    Authors and Affiliations

    1. Instituto de Investigaciones en Fruticultura Tropical, P.O. Box 11 300, 7th Ave. #3005, Playa, Havana, Cuba

      Lester Hernández-Rodríguez, Juana María Pérez-Castro, Victoria Zamora-Rodríguez, Xenia Ferriol-Marchena, Inés Peña-Bárzaga & Lochy Batista-Le Riverend

    2. Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n, Col. Chamilpa, 62210, Cuernavaca, Mexico

      Gabriel García-García

    3. Centro de Citricultura Sylvio Moreira, IAC, Caixa Postal 04, 13490-970, Cordeirópolis, SP, Brazil

      Pedro Luis Ramos-González

    Authors
    1. Lester Hernández-Rodríguez
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    2. Juana María Pérez-Castro
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    3. Gabriel García-García
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    4. Pedro Luis Ramos-González
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    5. Victoria Zamora-Rodríguez
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    7. Inés Peña-Bárzaga
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    Correspondence to Lester Hernández-Rodríguez.

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    Section Editor: Alice Inoue-Nagata

    Lester Hernández-Rodríguez and Juana María Pérez-Castro contributed equally to this work.

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    Hernández-Rodríguez, L., Pérez-Castro, J.M., García-García, G. et al. Citrus leaf blotch virus in Cuba: first report and partial molecular characterization. Trop. plant pathol. 41, 147–154 (2016). https://doi.org/10.1007/s40858-016-0078-4

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    • DOI: https://doi.org/10.1007/s40858-016-0078-4

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