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In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review

生物可降解镁基支架细胞相容性的体内外评价: 综述

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Abstract

Biodegradable magnesium (Mg)-based vascular stents have been designed as temporary scaffolds to treat angiostenotic lesions for the maintenance of normal blood flow. Numerous studies have presented in vitro and in vivo tests for the evaluation of the safety and feasibility of Mg-based vascular stents and the related materials. Therein the cytocompatibility is a basic and important parameter in the evaluation system. In this review, we summarize the applications and limitations of in vitro evaluation methods including basic characterization methods and direct and indirect cytotoxicity tests. We discuss the influencing factors on cytotoxicity, such as surface roughness, preconditioning of sample surface, cell type for the biocompatibility evaluation in direct contact as well as conditions for the formation of extracts/degradation products for indirect assays. Besides, we highlight the recent in vivo animal tests and clinical trials about Mg-based stents along with some associated results. The aim of this review is to provide a meaningful reference in the further developments and related evaluation methods of Mg-based stents.

摘要

生物可降解镁基血管支架具有临时支撑血管的作用, 用来治疗血管狭窄病变, 维持正常血流. 目前许多研究者已经报道了关于体内外评价镁基支架或其相关材料的生物安全性和应用可行性, 其中, 细胞相容性在这些评价系统中是一项非常重要的基本参数. 本文总结了包括直接法和间接法在内的体外评价方法的应用和缺陷, 以及表征细胞毒性的方法, 如MTT 和XTT等. 我们也讨论了体外细胞毒性的一些 影响因素, 在直接培养法中包括样品表面粗糙度、 样品表面预处理、 所采用的细胞类型等; 在间接法中有样品表面积和浸提溶液体积比值、 培养基中的血清浓度、 浸提液中的离子积累. 此外, 我们也列出了目前关于镁基支架的体内动物实验和临床试验及其相关的结果. 本综述的目的是希望能为将来镁基支架的研发和所涉及到的评价方法提供有意义的参考.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31600766, 21473138 and 81330031), and the Fundamental Research Funds for the Central Universities (No. 2682016CX076).

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Authors and Affiliations

  1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China

    Ping Li  (李萍), Ningling Zhou  (周宁玲), Hua Qiu  (邱华), Manfred F. Maitz, Juan Wang  (王娟) & Nan Huang  (黄楠)

  2. Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, 01069, Germany

    Manfred F. Maitz

  3. Department of Anesthesiology, School of Medicine, Yale University, New Haven, CT, 06511, USA

    Juan Wang  (王娟)

Authors
  1. Ping Li  (李萍)
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  2. Ningling Zhou  (周宁玲)
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  3. Hua Qiu  (邱华)
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  4. Manfred F. Maitz
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  5. Juan Wang  (王娟)
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  6. Nan Huang  (黄楠)
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Corresponding authors

Correspondence to Juan Wang  (王娟) or Nan Huang  (黄楠).

Additional information

Ping Li was born in 1992. She is now pursuing her master degree in the School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan. Her research interest is about the optimization of evaluation methods of biodegradable magnesium-based stent.

Juan Wang is currently a postdoctoral fellow at the School of Medicine, Yale University. She was an associate research scientist at the School of Materials and Engineering, Southwest Jiaotong University. She received her PhD degree in material science from Southwest Jiaotong University. She was a joint-PhD student at National Science Foundation Engineering Research Center for Revolutionizing Metallic Biomaterials, NC, US. Her research interests mainly focus on absorbable metallic vascular stent, and tissue engineering vascular graft, bioreactors, etc.

Nan Huang is a full professor at Southwest Jiaotong University. He received his master degree in materials science from Southwest Jiaotong University in 1985, and worked as research fellow and Guest Professor in University of Erlangen, Germany and Rossendorf Research Center, Germany from 1989–1991 and 1998–1999 respectively. His research interests include surface and interface of biomaterials, biodegradable biomaterials, cardiovascular devices. He is an inventor of a stent which has been applied in clinic.

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Li, P., Zhou, N., Qiu, H. et al. In vitro and in vivo cytocompatibility evaluation of biodegradable magnesium-based stents: a review. Sci. China Mater. 61, 501–515 (2018). https://doi.org/10.1007/s40843-017-9194-y

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  • DOI: https://doi.org/10.1007/s40843-017-9194-y

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