Abstract
It is known that mitochondrial population of living organisms is heterogeneous. Using the density gradient of Percoll or sucrose, we obtained and characterized two distinct mitochondrial subfractions (named “light” and “heavy” according to their sedimentation) from three plant species (Brassica rapa, Zea mays, and Arabidopsis thaliana). Electron microscopy showed that mitochondria of a light subfraction have a poorly developed cristae structure. The respiratory control of these mitochondria was reduced; however the degree of their outer membrane integrity remained high. These data suggest that mitochondrial subfractions purified in the density gradient differ in their maturity. We carried out DNA import assays to study the ability of these two subfractions for DNA transport. We have shown that the mitochondria of light subfraction in all three plant species uptakes DNA more efficiently than the mitochondria of heavy subfraction. In addition, import of DNA with different sizes into the light and heavy mitochondrial subfractions has different sensitivity to specific inhibitors of the voltage-dependent anion channel and adenine nucleotide translocase, proteins playing a central role in DNA transport across the mitochondrial membrane. The obtained results suggest that DNA import depends on organization of the transport system in the double mitochondrial membrane influenced by the maturity of the mitochondria.
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Acknowledgements
DNA import assays using the radioactively labeled DNA substrates were performed in the Andre Dietrich research group at the Institute of Plant Molecular Biology, CNRS, Strasbourg, France. Equipment of the Bioanalitika Center for Collective Use, Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, Irkutsk, was used in this study. This work was financially supported by the Russian Foundation for Basic Research (projects 18-04-00603, 20-44-383003, 20-34-80011).
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Tarasenko, T.A., Subota, I.Y., Tarasenko, V.I. et al. Plant mitochondrial subfractions have different ability to import DNA. Theor. Exp. Plant Physiol. 32, 5–18 (2020). https://doi.org/10.1007/s40626-020-00167-w
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DOI: https://doi.org/10.1007/s40626-020-00167-w