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An aptamer-based colorimetric sensor for streptomycin and its application in food inspection

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Abstract

An aptamer-based colorimetric biosensor was developed, which could be used to detect residual streptomycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using systemic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A 620/A 520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules analogues in food inspection.

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Authors and Affiliations

  1. College of Life Sciences, Hebei University, Baoding, 071002, P. R. China

    Zhongcheng Liu & Wufang Fan

  2. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding, 071002, P. R. China

    Zhongcheng Liu, Yao Xie, Kewei Bi, Zhe Cui & Lijian Zhao

  3. Office of Science and Technology, Hebei University, Baoding, 071002, P. R. China

    Yanfen Zhang & Ying Sun

Authors
  1. Zhongcheng Liu
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  2. Yanfen Zhang
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  3. Yao Xie
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  4. Ying Sun
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  5. Kewei Bi
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  6. Zhe Cui
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  7. Lijian Zhao
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  8. Wufang Fan
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Corresponding author

Correspondence to Wufang Fan.

Additional information

Supported by the National Natural Science Foundation of China(No.81202338), the Natural Science Foundation of Hebei Province, China(No.H2016201121), the National Training Program of Innovation and Entrepreneurship for Undergraduates, China(No.201610075001), the Postdoctoral Science Foundation of China(No.2013M530885) and the Science and Technology Research Project of Hebei Higher Education Institutions, China(No.ZD2017010).

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Liu, Z., Zhang, Y., Xie, Y. et al. An aptamer-based colorimetric sensor for streptomycin and its application in food inspection. Chem. Res. Chin. Univ. 33, 714–720 (2017). https://doi.org/10.1007/s40242-017-7029-6

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  • DOI: https://doi.org/10.1007/s40242-017-7029-6

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