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Potential of H1N1 influenza A virus as an air borne pathogen to induce infectivity in pancreas: a mouse model study

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Abstract

Introduction

H1N1 influenza virus, as an indoor/outdoor pathogen in air, can cause the flu-like illness and respiratory complication. The aim of this study was to evaluate the H1N1 influenza virus replication in pancreas and investigate the immune response against infected pancreas.

Material and methods

First, mouse pancreas cell line was infected by H1N1 influenza A virus using intranasally and intravenously infection methods, and then the pancreas tissue was collected and pathology experiment was carried out. Next, the protein and genome of influenza virus were detected using immunocytochemistry and real-time PCR, respectively. In addition, serum cytokines and serum lipase were investigated using ELISA.

Result

The in-vitro results proved that the mouse pancreatic cell line can support influenza virus replication. The result also proved that influenza virus is capable to infect pancreas and induce pancreas damage. Further, the immune response in mice with infected pancreas exhibited a completely different pattern with that of mice infected through intranasal method.

Conclusion

It can be concluded that influenza virus can infect pancreas and change the influenza disease pathway, which might result in a pancreatic injury.

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References

  1. López HC, Roca R, Daunis J. Pneumonia and the acute respiratory distress syndrome due to influenza a (H1N1) virus. Med Int. 2009;33(9):455–8.

    Google Scholar 

  2. Blum A, Podvitzky O, Shalabi R, Simsolo C. Acute pancreatitis may be caused by H1N1 influenza a virus infection. Isr Med Assoc J: IMAJ. 2010;12(10):640–1.

    Google Scholar 

  3. Calore EE, Uip DE, Perez NM. Pathology of the swine-origin influenza A (H1N1) flu. Pathol Res Pract. 2011;207(2):86–90.

    Article  CAS  Google Scholar 

  4. Oikarinen S, Tauriainen S, Hober D, Lucas B, Vazeou A, Sioofy-Khojine A, et al. Virus antibody survey in different European populations indicates risk association between coxsackievirus B1 and type 1 diabetes. Diabetes. 2014;63(2):655–62.

    Article  CAS  Google Scholar 

  5. Gale E. Congenital rubella: citation virus or viral cause of type 1 diabetes? Berlin: Springer; 2008.

    Google Scholar 

  6. Aarnisalo J, Veijola R, Vainionpää R, Simell O, Knip M, Ilonen J. Cytomegalovirus infection in early infancy: risk of induction and progression of autoimmunity associated with type 1 diabetes. Diabetologia. 2008;51(5):769–72.

    Article  CAS  Google Scholar 

  7. Habib A, Jain A, Singh B, Jamshed N. H1N1 influenza presenting as severe acute pancreatitis and multiorgan dysfunction. Am J Emerg Med. 2016;34(9):1911.e1–2.

    Article  Google Scholar 

  8. Rodríguez DS, Martínez A, Guzmán M, Robledo H, Capocasa P, Martinez L, et al. Severe acute pancreatitis and infection by influenza A (H1N1) virus in a child: case report. Arch Argent Pediatr. 2015;113(4):e215–8.

    Google Scholar 

  9. Sánchez BA, Alcalá MP, Segura SS, Mira-Perceval JG. 2009 pandemic influenza A (H1N1) virus infection treated with oseltamivir and possible association with acute pancreatitis in a 12 years old patient. Enferm Infecc Microbiol Clin. 2015;33(2):139.

    Article  Google Scholar 

  10. Ruiz PL, Tapia G, Bakken IJ, Håberg SE, Hungnes O, Gulseth HL, et al. Pandemic influenza and subsequent risk of type 1 diabetes: a nationwide cohort study. Diabetologia. 2018;61(9):1996–2004.

    Article  Google Scholar 

  11. Cavicchioli L, Zappulli V, Beffagna G, Caliari D, Zanetti R, Nordio L, et al. Histopathological and immunohistochemical study of exocrine and endocrine pancreatic lesions in avian influenza A experimentally infected turkeys showing evidence of pancreatic regeneration. Avian Pathol. 2015;44(6):498–508.

    Article  Google Scholar 

  12. Van Poucke SG, Nicholls JM, Nauwynck HJ, Van Reeth K. Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution. Virol J. 2010;7(1):38.

    Article  Google Scholar 

  13. Raman R, Tharakaraman K, Shriver Z, Jayaraman A, Sasisekharan V, Sasisekharan R. Glycan receptor specificity as a useful tool for characterization and surveillance of influenza A virus. Trends Microbiol. 2014;22(11):632–41.

    Article  CAS  Google Scholar 

  14. Huo C, Xiao K, Zhang S, Tang Y, Wang M, Qi P, et al. H5N1 influenza A virus replicates productively in pancreatic cells and induces apoptosis and pro-inflammatory cytokine response. Front Cell Infect Microbiol. 2018;8:386.

    Article  CAS  Google Scholar 

  15. Gukovskaya AS, Vaquero E, Zaninovic V, Gorelick FS, Lusis AJ, Brennan ML, et al. Neutrophils and NADPH oxidase mediate intrapancreatic trypsin activation in murine experimental acute pancreatitis. Gastroenterology. 2002;122(4):974–84.

    Article  CAS  Google Scholar 

  16. Abdulla A, Awla D, Thorlacius H, Regnér S. Role of neutrophils in the activation of trypsinogen in severe acute pancreatitis. J Leukoc Biol. 2011;90(5):975–82.

    Article  CAS  Google Scholar 

  17. Eisfeld AJ, Neumann G, Kawaoka Y. Influenza A virus isolation, culture and identification. Nat Protoc. 2014;9(11):2663–81.

    Article  CAS  Google Scholar 

  18. Capua I, Mercalli A, Pizzuto MS, Romero-Tejeda A, Kasloff S, De Battisti C, et al. Influenza A viruses grow in human pancreatic cells and cause pancreatitis and diabetes in an animal model. J Virol. 2013;87(1):597–610.

    Article  CAS  Google Scholar 

  19. Miller AM. Role of IL-33 in inflammation and disease. J Inflamm. 2011;8(1):22.

    Article  CAS  Google Scholar 

  20. Sesti-Costa R, Silva GK, Proença-Módena JL, Carlos D, Silva ML, Alves-Filho JC, et al. The IL-33/ST2 pathway controls Coxsackievirus B5–induced experimental pancreatitis. J Immunol. 2013;191(1):283–92.

    Article  CAS  Google Scholar 

  21. Eyles JL, Hickey MJ, Norman MU, Croker BA, Roberts AW, Drake SF, et al. A key role for G-CSF–induced neutrophil production and trafficking during inflammatory arthritis. Blood, The Journal of the American Society of Hematology. 2008;112(13):5193–201.

    CAS  Google Scholar 

  22. Velazquez-Salinas L, Verdugo-Rodriguez A, Rodriguez LL, Borca MV. The role of interleukin 6 during viral infections. Front Microbiol. 2019;10:1057.

    Article  Google Scholar 

  23. Tanaka T, Narazaki M, Kishimoto T. IL-6 en la inflamación, la inmunidad, y la enfermedad. Cold Spring Harb Perspect Biol. 2014;6(10):a016295.

    Article  Google Scholar 

  24. Crowe CR, Chen K, Pociask DA, Alcorn JF, Krivich C, Enelow RI, et al. Critical role of IL-17RA in immunopathology of influenza infection. J Immunol. 2009;183(8):5301–10.

    Article  CAS  Google Scholar 

  25. Sanjabi S, Zenewicz LA, Kamanaka M, Flavell RA. Anti-inflammatory and pro-inflammatory roles of TGF-β, IL-10, and IL-22 in immunity and autoimmunity. Curr Opin Pharmacol. 2009;9(4):447–53.

    Article  CAS  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge the School of Public Health, Tehran university of Medical Science for providing financial support. This study was extracted from Ph.D. Dissertation. This study was funded by School of Public Health, Tehran university of Medical Science (grant number: 97-02-27-38327).

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Authors and Affiliations

  1. Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Kaveh Sadeghi, Vahid Salimi, Farhad Rezaei, Nastaran Ghavami & Talat Mokhtari Azad

  2. Department of Medical Virology, Hamedan University of Medical Sciences, Hamadan, Iran

    Farid azizi Jalilian

Authors
  1. Kaveh Sadeghi
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  2. Vahid Salimi
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  3. Farhad Rezaei
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  4. Farid azizi Jalilian
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  5. Nastaran Ghavami
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  6. Talat Mokhtari Azad
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Correspondence to Talat Mokhtari Azad.

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Sadeghi, K., Salimi, V., Rezaei, F. et al. Potential of H1N1 influenza A virus as an air borne pathogen to induce infectivity in pancreas: a mouse model study. J Environ Health Sci Engineer 18, 303–310 (2020). https://doi.org/10.1007/s40201-020-00468-x

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  • DOI: https://doi.org/10.1007/s40201-020-00468-x

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