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Acceleration of a delayed healing wound repair model in diabetic rats by additive impacts of photobiomodulation plus conditioned medium of adipose-derived stem cells

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Abstract

Purpose

This study aimed to investigate the effects of photobiomodulation (PBM) and conditioned medium (CM) derived from human adipose-derived stem cells (h-ASCs), both individually and in combination, on the maturation stage of an ischemic infected delayed healing wound model (IIDHWM) in type I diabetic (TIDM) rats.

Methods

The study involved the extraction of h-ASCs from donated fat, assessment of their immunophenotypic markers, cell culture, and extraction and concentration of CM from cultured 1 × 10^6 h-ASCs. TIDM was induced in 24 male adult rats, divided into four groups: control, CM group, PBM group (80 Hz, 0.2 J/cm2, 890 nm), and rats receiving both CM and PBM. Clinical and laboratory evaluations were conducted on days 4, 8, and 16, and euthanasia was performed using CO2 on day 16. Tensiometrical and stereological examinations were carried out using two wound samples from each rat.

Results

Across all evaluated factors, including wound closure ratio, microbiological, tensiometrical, and stereological parameters, similar patterns were observed. The outcomes of CM + PBM, PBM, and CM treatments were significantly superior in all evaluated parameters compared to the control group (p = 0.000 for all). Both PBM and CM + PBM treatments showed better tensiometrical and stereological results than CM alone (almost all, p = 0.000), and CM + PBM outperformed PBM alone in almost all aspects (p = 0.000). Microbiologically, both CM + PBM and PBM exhibited fewer colony-forming units (CFU) than CM alone (both, p = 0.000).

Conclusion

PBM, CM, and CM + PBM interventions substantially enhanced the maturation stage of the wound healing process in IIDHWM of TIDM rats by mitigating the inflammatory response and reducing CFU count. Moreover, these treatments promoted new tissue formation in the wound bed and improved wound strength. Notably, the combined effects of CM + PBM surpassed the individual effects of CM and PBM.

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Funding

This study primarily received financial support from NIH grants R44DK133065 and R44DK105692.

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Authors and Affiliations

  1. Department of Paramedicine, Tehran University of Medical Sciences, Tehran, Iran

    Robabeh Asadi

  2. Department of Anatomical Sciences and Cognitive Neuroscience, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Atarodalsadat Mostafavinia

  3. Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abdollah Amini, Houssein Ahmadi, Behnaz Ahrabi, Hamidreza Omidi & Masoumeh Hajihosseintehrani

  4. School of Medicine, Islamic Azad University, Shahrood, Iran

    Erfan Pourhashemi

  5. College of Pharmacy, University of Kentucky, Lexington, KY, USA

    Fatemehalsadat Rezaei

  6. Department of Pathology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Zhaleh Mohsenifar

  7. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC of Louisville, Louisville, USA

    Sufan Chien & Mohammad Bayat

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  1. Robabeh Asadi
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Correspondence to Sufan Chien or Mohammad Bayat.

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Ethical considerations

The protocols, methods, and tests employed in this study were approved by the Ethics Committee at the School of Medicine of Shahid Beheshti University of Medical Sciences (SBMU.MSP.REC.1400.456).

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Asadi, R., Mostafavinia, A., Amini, A. et al. Acceleration of a delayed healing wound repair model in diabetic rats by additive impacts of photobiomodulation plus conditioned medium of adipose-derived stem cells. J Diabetes Metab Disord 22, 1551–1560 (2023). https://doi.org/10.1007/s40200-023-01285-3

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