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Association of ADIPOQ rs266729 and rs1501299 gene polymorphisms and circulating adiponectin level with the risk of type 2 diabetes in a population of Iran: a case-control study

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Abstract

Purpose

Mutation in various regions of the adiponectin (ADIPOQ) gene is described to be associated with type 2 diabetes (T2D). Our aim was to investigate the association between rs1501299 (SNP + 276 G > T) and rs266729 (SNP-11377 C > G) polymorphisms of ADIPOQ gene and susceptibility to T2D in an Iranian population.

Methods

This is a case-control study. A total of 210 subjects including 100 patients with T2D and 110 healthy controls were selected from an Iranian population. SNP-11377 C > G and SNP + 276 G > T polymorphisms were investigated using the Tetra ARMS-PCR method.

Results

The genotypes frequency of the SNP-11377 C > G polymorphism (P = 0.21) and SNP + 276 G > T polymorphism (P = 0.10) were in agreement with the Hardy-Weinberg equilibrium. The genotype and allele frequencies for rs266729 polymorphism showed a significant difference between the T2D and control groups. Further, the frequencies of the genotypes of GG (OR = 2.43, P = 0.031), GG + GG (OR = 2.11, P < 0.01) and G allele (OR = 1.6, P = 0.041) in SNP-11377 C > G polymorphism was significantly associated with an increased risk of T2D compared to control group. While, there was no significant association between the different genotypes of SNP + 276 G > T polymorphism and the risk of T2D in an Iranian population. The circulating adiponectin level in T2D subjects had significant difference compared to the control group in rs266729 polymorphism.

Conclusion

We concluded that ADIPOQ rs266729 (SNP-11377 C > G) gene polymorphism but no rs1501299 is associated with increased risk of T2D in our population.

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Abbreviations

Tetra ARMS-PCR:

tetra primer amplification refractory mutation system-polymerase chain reaction

T2D:

type 2 diabetes

WHO:

world health organization

ADIPOQ:

the gene coding of adiponectin

FPG:

fasting plasma glucose

TG:

triglyceride

HDL-C:

high- density lipoprotein-cholesterol

LDL-C:

low-density lipoprotein- cholesterol

HWE:

Hardy-Weinberg Equilibrium

HOMA-IR:

homeostatic model assessment of insulin resistance

GDM:

gestational diabetes mellitus

NAFLD:

non-alcoholic fatty liver disease.

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Acknowledgements

We would like to thank the staff of Department of chemistry, Shahrood Branch, Islamic Azad University.

Availability of data and materials

Data of this study are included in the article and the primary data can be provided from the corresponding author.

Funding

The authors received no specific funding for this work. This is a part of the results of first author’s Ph.D thesis (M.A).

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Authors and Affiliations

  1. Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, IR, Iran

    Mahrokh Alimi & Mehdi Nekoei

  2. Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, IR, Iran

    Mohammad Taghi Goodarzi

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  1. Mahrokh Alimi
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Contributions

MA and MT.G conceptualized and designed the research, MT.G and MN critically reviewed the manuscript and approved the final manuscript as submitted. MA and MT.G wrote the draft of this paper and all authors have directly participated in the Data collection, statistical analyses and Data interpretation.

Corresponding author

Correspondence to Mohammad Taghi Goodarzi.

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The experiments were approved by the Ethics Committee of Department of chemistry, Shahrood Branch, Islamic Azad University. Written informed consent was obtained from every participant.

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Alimi, M., Goodarzi, M.T. & Nekoei, M. Association of ADIPOQ rs266729 and rs1501299 gene polymorphisms and circulating adiponectin level with the risk of type 2 diabetes in a population of Iran: a case-control study. J Diabetes Metab Disord 20, 87–93 (2021). https://doi.org/10.1007/s40200-020-00715-w

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