Abstract
In the present work, a commercially used azo dye Remazol navy blue was completely degraded into less toxic intermediates in an integrated microbial fuel cell-aerobic system. A novel defined bacterial consortium comprising of organisms isolated from dye contaminated wastewater has been used as inoculum for this study. As compared to the traditional static incubation method, a rapid decolorization of Remazol navy blue was noticed in the anodic chamber of microbial fuel cell. In low to moderate dye concentrations (25–100 mg/L), almost complete decolorization of Remazol navy blue was achieved within 12 h of microbial fuel cell operation. The first-order kinetic constant of Remazol navy blue decolorization (k) in microbial fuel cell was found to be considerably higher than that of static incubation condition (Kstatic (0.3041) < KMFC (0.5697)). Microbial fuel cells external resistance is found to be a key parameter affecting the reductive decolorization of Remazol navy blue dye with a resistance of 1000 Ω as the optimum giving maximum efficiency. Dye degradation products from each stage of the integrated microbial fuel cell-aerobic system have been analyzed through different analytical techniques such as UV–Vis spectroscopy, Fourier transform infrared spectroscopy and gas chromatography–mass spectrometry analysis. These studies illustrate the reductive degradation of Remazol navy blue in microbial fuel cell treatment stage into different toxic intermediates which were further degraded into simpler compounds in the successive aerobic treatment stage. Phototoxicity study confirms the less toxic nature of the treated effluents as compared to the parent dye.
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References
à ZY, Wen X (2005) Screening and identification of yeasts for decolorizing synthetic dyes in industrial wastewater. Int Biodeterior Biodegrad 56:109–114. https://doi.org/10.1016/j.ibiod.2005.05.006
à AP, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegrad 59:73–84. https://doi.org/10.1016/j.ibiod.2006.08.006
Albuquerque MGE et al (2005) Biological sulphate reduction and redox mediator effects on azo dye decolourisation in anaerobic-aerobic sequencing batch reactors. Enzyme Microb Technol 36(5–6):790–799. https://doi.org/10.1016/j.enzmictec.2005.01.005
Babu BR et al (2007) Cotton textile processing: waste generation and effluent treatment. J Cotton Sci 153(11:141):141–153
Du Z, Li H, Gu T (2007) A state of the art review on microbial fuel cells: a promising technology for wastewater treatment and bioenergy. Biotechnol Adv 25:464–482. https://doi.org/10.1016/j.biotechadv.2007.05.004
Fernando E, Keshavarz T, Kyazze G (2012) Enhanced bio-decolourisation of acid orange 7 by Shewanella oneidensis through co-metabolism in a microbial fuel cell. Int Biodeterior Biodegrad 72:1–9. https://doi.org/10.1016/j.ibiod.2012.04.010
Fernando E, Keshavarz T, Kyazze G (2014a) Complete degradation of the azo dye Acid Orange-7 and bioelectricity generation in an integrated microbial fuel cell, aerobic two-stage bioreactor system in continuous flow mode at ambient temperature. Biores Technol 156:155–162
Fernando E, Keshavarz T, Kyazze G (2014b) External resistance as a potential tool for in fl uencing azo dye reductive decolourisation kinetics in microbial fuel cells. Int Biodeterior Biodegrad 89:7–14. https://doi.org/10.1016/j.ibiod.2013.12.011
Fu Y, Viraraghavan T (2001) Fungal decolorization of dye wastewaters: a review. Bioresour Technol . https://doi.org/10.1016/s0960-8524(01)00028-1
Gottlieb A et al (2003) The toxicity of textile reactive azo dyes after hydrolysis and decolourisation. J Biotechnol 101:49–56
Hano T (2005) Treatment of azo dye Orange II in a sequential anaerobic and aerobic-sequencing batch ractor system. Environ Chem Lett. https://doi.org/10.1007/s10311-004-0098-z
Kalathil S, Lee J, Cho MH (2012) Efficient decolorization of real dye wastewater and bioelectricity generation using a novel single chamber biocathode-microbial fuel cell. Biores Technol 119:22–27
Kant R (2012) Textile dyeing industry an environmental hazard. Nat Sci 4(1):22–26. https://doi.org/10.4236/ns.2012.41004
Kosalec I, Ramić S, Jelić D, Antolović R, Pepeljnjak S, Kopjar N (2011) Assessment of tryptophol genotoxicity in four cell lines in vitro: a pilot study with alkaline comet assay. Arch Ind Hyg Toxicol 62:41–49. https://doi.org/10.2478/10004-1254-62-2011-2090
Lade HS et al (2012) Enhanced biodegradation and detoxification of disperse azo dye Rubine GFL and textile industry effluent by defined fungal-bacterial consortium. Int Biodeterior Biodegrad 72:94–107. https://doi.org/10.1016/j.ibiod.2012.06.001
Li Z et al (2010) Azo dye treatment with simultaneous electricity production in an anaerobic—aerobic sequential reactor and microbial fuel cell coupled system. Biores Technol 101:4440–4445. https://doi.org/10.1016/j.biortech.2010.01.114
Lourenço ND, Novais JM, Pinheiro HM (2006) Kinetic studies of reactive azo dye decolorization in anaerobic/aerobic sequencing batch reactors. Biotech Lett 28(10):733–739. https://doi.org/10.1007/s10529-006-9051-5
Maria F et al (2013) Textile dyes: dyeing process and environmental impact. InTech, Rijeka. https://doi.org/10.5772/53659
Miran W et al (2016) Simultaneous electricity production and Direct Red 80 degradation using a dual chamber microbial fuel cell. Desalin Water Treat 3994(September):1–9. https://doi.org/10.1080/19443994.2015.1049410
Morris JM et al (2009) Microbial fuel cell in enhancing anaerobic biodegradation of diesel. Chem Eng J 146:161–167. https://doi.org/10.1016/j.cej.2008.05.028
Phugare SS et al (2011) Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. J Hazard Mater 186:713–723. https://doi.org/10.1016/j.jhazmat.2010.11.049
REHN L (1895) Blasengeschwulste bei Fuchsin-Arbeitern. Arch Klin Chir 50:588–600. http://ci.nii.ac.jp/naid/10007051320/en/, Accessed 21 Sept 2016
Saratale RG et al (2009) Ecofriendly degradation of sulfonated diazo dye C. I. Reactive Green 19A using Micrococcus glutamicus NCIM-2168. Biores Technol 100:3897–3905. https://doi.org/10.1016/j.biortech.2009.03.051
Singh RP, Singh PK, Singh RL (2014) Bacterial decolorization of textile azo dye acid orange by Staphylococcus hominis RMLRT03. Toxicol Int 21(2):160–166. https://doi.org/10.4103/0971-6580.139797
Sun J et al (2009) Simultaneous decolorization of azo dye and bioelectricity generation using a microfiltration membrane air-cathode single-chamber microbial fuel cell. Biores Technol 100:3185–3192. https://doi.org/10.1016/j.biortech.2009.02.002
Tan NCG et al (2005) Fate and biodegradability of sulfonated aromatic amines. Biodegradation 16(6):527–537
Taylor P, Prasad SS, Aikat K (2013) Optimization of medium for decolorization of Congo red by Enterobacter sp. SXCR using response surface methodology. Desalin Water Treat. https://doi.org/10.1080/19443994.2013.812525
Van der Zee FP, Lettinga G, Field JA (2001) Azo dye decolourisation by anaerobic granular sludge. Chemosphere 44(5):1169–1176. https://doi.org/10.1016/S0045-6535(00)00270-8
Verstraete W, Rabaey K (2006) Critical review microbial fuel cells: methodology and technology. Environ Sci Technol 40(17):5181–5192
Vijayaraghavan J, Sardhar Basha SJ, Jegan J (2013) A review on efficacious methods to decolorize reactive azo dye. J Urban Environ Eng 7(1):30–47. https://doi.org/10.4090/juee.2013.v7n1.030047
Wibbertmann A, Kielhorn J, Koennecker G, Mangelsdorf I, Melber C (2005) Benzoic acid and sodium benzoate
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This research work was financially supported by National Institute of Technology, Rourkela, India.
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Das, A., Mishra, S. Complete biodegradation of azo dye in an integrated microbial fuel cell-aerobic system using novel bacterial consortium. Int. J. Environ. Sci. Technol. 16, 1069–1078 (2019). https://doi.org/10.1007/s13762-018-1703-1
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DOI: https://doi.org/10.1007/s13762-018-1703-1