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Improvement of monacolin K/citrinin production ratio in Monascus purpureus using UV mutagenesis

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Nutrafoods

Abstract

Monascus purpureus MTCC 1090 is a fungus that produces monacolin K, gamma-aminobutyric acid (GABA) and citrinin under solid-state and submerged fermentation. The production of citrinin has a negative impact on its acceptance as a nutraceutical due to its neurotoxic, hepatotoxic and genotoxic effects. The purpose of the present study is to produce a M. purpureus mutant with zero concentration of citrinin production by random mutagenesis using ultra-violet (UV) irradiation. Mutants were screened on the basis of changes in their cultural characteristics and ratio of inhibition against Bacillus subtilis by the cross-streak method. Mutant M. purpureus 254 produced no citrinin as analysed by high-performance liquid chromatography and showed an increase in monacolin K production of about 74.32% in an optimised fermentation medium.

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Authors and Affiliations

  1. Indian Pharmacopoeia Commission Ministry of Health and Family Welfare, Govt. of India, Sector-23, Raj Nagar, Ghaziabad, 201 002, Uttar Pradesh, India

    M. Kalaivani

  2. KMCH College of Pharmacy, Coimbatore, Tamil Nadu, India

    A. Rajasekaran

Authors
  1. M. Kalaivani
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  2. A. Rajasekaran
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Correspondence to M. Kalaivani.

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Kalaivani, M., Rajasekaran, A. Improvement of monacolin K/citrinin production ratio in Monascus purpureus using UV mutagenesis. Nutrafoods 13, 79–84 (2014). https://doi.org/10.1007/s13749-014-0021-6

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  • DOI: https://doi.org/10.1007/s13749-014-0021-6

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