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Valutazione del sistema Cellavision DM-96 nell’analisi della formula leucocitaria e nel conteggio piastrinico

Cellavision DM-96: white blood cell morphological evaluation and platelet count

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La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine

Riassunto

Premesse.

L’impiego di contaglobuli automatici per il conteggio dei globuli bianchi (WBC), globuli rossi (RBC) e piastrine (PLT) rappresenta ormai il sistema di analisi più diffuso nei Laboratori e, nonostante i progressi diagnostici degli ultimi anni, la lettura manuale dello striscio di sangue periferico rimane ancora il riferimento metodologico per una rapida e accurata diagnostica delle malattie ematologiche. Nuovi strumenti automatici sono stati recentemente introdotti per migliorare la diagnostica microscopica, riducendo i tempi di esecuzione e le potenziali variabili analitiche operatore dipendente della metodica manuale. Scopo del presente lavoro è confrontare la metodica manuale in microscopia ottica e il metodo semiautomatizzato Cellavision (Cellavision, Lund, Sweden) nella lettura dello striscio di sangue periferico e nel conteggio delle piastrine.

Metodi.

Sono stati selezionati 116 campioni consecutivi di pazienti ricoverati (44 maschi e 72 femmine di età compresa tra 1 e 99 anni) non validati automaticamente dai sistemi Sysmex (Sysmex, Kobe, Japan) e quindi esaminati sia in microscopia ottica sia tramite Cellavision. Per la valutazione dei conteggi piastrinici sono stati analizzati 131 campioni consecutivi di pazienti ricoverati (67 maschi e 64 femmine di età compresa tra 2 e 93 anni) non validati automaticamente e che non presentavano il segnale di allarme per la presenza di aggregati piastrinici.

Risultati.

Le correlazioni (r) tra le diverse sottopopolazioni leucocitarie, valutate tramite Cellavision e microscopio ottico, sono comprese tra un massimo di 0,99 e un minimo di 0,62. Dall’analisi dei tempi di esecuzione si evidenzia che il Cellavision risulta circa 4,9 volte più veloce rispetto alla classica microscopia ottica nella lettura degli strisci ematologici. Un’ottima correlazione tra Sysmex XE-2100 e Cellavision si è evidenziata relativamente al conteggio delle PLT.

Conclusioni.

Lo studio ha mostrato che il Cellavision può essere un utile strumento all’interno di un moderno Laboratorio di Ematologia a supporto della microscopia ottica tradizionale.

Summary

Background.

Automated haematology systems for the complete blood cell count (BCC) are widely used in clinical laboratories with undoubtfully advantages. Despite the introduction of innovative technologies in the last decades, the accuracy of automated BCC is still limited in pathological cases, with the consequence that traditional optic microscopic analysis is required to properly classify white blood cells (WBC), red blood cells (RBC) and platelets (PLT). Aim of the study was to compare the traditional microscopic analysis with Cellavision (Cellavision, Lund, Sweden) in WBC morphological evaluation. Moreover, we analysed the agreement between PLT count derived from automatic Sysmex XE-2100 (Sysmex, Kobe, Japan) and Cellavision.

Methods.

116 consecutive blood samples (44 males and 72 females; aged 1–99 years), automatically analysed and not validated by Sysmex XE-2100 were evaluated both with Cellavision and manual optical microscopy. 131 consecutive blood samples (67 males and 64 females; aged 2–93 years) were analysed to compare PLT counts both in Sysmex XE-2100 and Cellavision.

Results.

The correlation between Cellavision and traditional microscopy in the different WBC populations varies from r=0.62 to r=0.99. We showed an advantage of Cellavision in reducing the time spent for a single morphological evaluation. A high correlation between Sysmex XE-2100 and Cellavision was also demonstrated in PLT count.

Conclusions.

The study showed that Cellavision can be usefully integrated in a modern haematology Laboratory in support of traditional microscopy.

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Authors and Affiliations

  1. UO Laboratorio Analisi Chimico Cliniche e Microbiologiche, Centro Emostasi e Trombosi, AO Istituti Ospitalieri, Viale Concordia 1, 26100, Cremona, Italia

    Maurizio Tala, Oriana Paoletti, Maria Annunciata Medagliani, Rossella Morandini, Anke Zimmermann, Claudia Dellanoce, Emilia Cancellieri & Sophie Testa

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  1. Maurizio Tala
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  2. Oriana Paoletti
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  3. Maria Annunciata Medagliani
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  4. Rossella Morandini
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  6. Claudia Dellanoce
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  7. Emilia Cancellieri
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  8. Sophie Testa
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Correspondence to Sophie Testa.

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Tala, M., Paoletti, O., Medagliani, M.A. et al. Valutazione del sistema Cellavision DM-96 nell’analisi della formula leucocitaria e nel conteggio piastrinico. Riv Ital Med Lab 11, 111–117 (2015). https://doi.org/10.1007/s13631-015-0085-3

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