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Anti-arthritic potentials of aqueous and methanolic leaf extracts of Ardisia solanacea on complete Freund’s adjuvant induced rheumatoid arthritis in rats

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Abstract

Ardisia solanacea is a well-known herb with a significant medicinal value. Traditionally, the plant extracts have commonly been used as an immunomodulator, antimicrobial, thrombolytic, anthelminthic, and cytotoxic agent in India. The objective of this study was to assess the in-vivo antiarthritic potential of A. solanacea in the adjuvant-induced arthritic rat model. The rats were divided into nine groups (n = 6). They were administered with complete freunds adjuvant (CFA) in the right hind paw and treated with different doses (250 mg/kg, 500 mg/kg) of the leaf extract orally from day one to day 21st. The standard group was treated with 10 mg/kg body weight of diclofenac sodium. Throughout the treatment, we monitored the differences in paw edema, body weight (BW), and arthritis index in different groups of animals. Besides, we also have evaluated the tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, C-reactive protein, rheumatoid factor (RF), and hematology. Further, we have assessed the severity of arthritis through radiography and histopathology of ankle joints. In addition, we also have studied liver and kidney histology. The arthritic symptoms were subsided significantly (p < 0.01) in the test extract treated groups at a higher dose. We also observed a downregulation of cytokines in the ASM and ASW treated group compared to the negative control. Furthermore, radiography and histopathology revealed improved prognosis in ASM and ASW administered groups. Methanolic and aqueous extract of Ardisia solanacea exhibited potential antiarthritic effect against CFA-induced arthritic rats.

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Acknowledgements

Siksha O Anusandhan, Deemed to be University for providing the infrastructure and research facility and to the team of Indquench Life Science Innovations (OPC) Pvt Ltd for their technical support.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Siksha “O” Anusandhan Deemed to be University, Bhubaneshwar, Odisha, 751003, India

    Lingarkar Silpavathi & Debajyoti Das

  2. IndQuench Life Science Innovations (OPC) Pvt. Ltd, Plot No: 31/761, Devika Bihar, Dasabatia, Tamando (P), Khurda (Dist), Bhubaneswar, Odisha, 751028, India

    Mukesh Kumar Das

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  1. Lingarkar Silpavathi
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  3. Debajyoti Das
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Correspondence to Debajyoti Das.

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The institutional animal ethical committee approved the protocol for the experiment (IAEC/SPS/SOA/23/2019).

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Lingarkar Silpavathi has no conflict of interest. Mukesh Kumar Das has no conflict of interest. Debajyoti Das has no conflict of interest.

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Silpavathi, L., Das, M.K. & Das, D. Anti-arthritic potentials of aqueous and methanolic leaf extracts of Ardisia solanacea on complete Freund’s adjuvant induced rheumatoid arthritis in rats. ADV TRADIT MED (ADTM) 23, 111–119 (2023). https://doi.org/10.1007/s13596-021-00591-6

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