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Evaluation of antacid capacity and antiulcer activity of Calendula officinalis L. in experimental rats

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Abstract

Calendula officinalis has been traditionally used in India for treating wounds, ulcers, herpes, scars, skin damage, frostbite and blood purification. Present study was aimed to evaluate antacid capacity in-vitro and antiulcer activity of extract from Calendula officinalis L. (family: Asteraceae) in experimental rats. Evaluation of ethanol extract (100 and 200 mg/kg, orally) from Calendula officinalis (ECO) as antisecretory and in-vivo antacid capacity were carried out by pyloric ligation induced ulcer model. The gastroprotective effect was carried out by absolute ethanol induced ulcer and indomethacin induced ulcer model. Integrity of gastric mucosa was evaluated by the estimation of glutathione (GSH) and gastric mucus level. The in-vitro antacid capacity was evaluated by titration method. ECO produced significant inhibition of ulcer index in all models of ulcers and comparable to standard. The in- vitro antacid capacity was found as 20.58 ± 0.08 mEq/g of ECO. The total protein and pepsin level were found as 92.45 ± 1.89 μg/ml and 34.67 ± 1.25 μmole/ml, respectively in pylorus ligation model at 200 mg/kg of ECO. GSH level were significant elevated by 200 mg/kg of ECO (P < 0.01) in indomethacin induced ulcers and shows superiority over standard drug (P < 0.05). Gastric wall mucus level was increased in all three models of ulcers and comparable to the ranitidine. We believe that the antiulcer activity of Calendula officinalis was due to its antisecretory and gastroprotective effect on experimental rats. Our results contribute towards validation of the traditional use of Calendula officinalis in the treatment ulcers.

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Acknowledgments

The authors wish to thank Prof (Dr.) R M. Dubey, Vice Chancellor, IFTM University for his generous support for the study. This study is a part of PhD from UPTU, Lucknow.

Ethical Statement

This study was approved by the Institutional animal ethics committee (IAEC) and performed in accordance with the ethical standard laid down in the 1964 Declaration of Helsinki and its later amendments.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, IFTM University, Lodhipur Rajput, Delhi Road (NH-24), Moradabad, 244 102, UP, India

    Phool Chandra & Ashoke Kumar Ghosh

  2. Department of Pharmacy, MJP Rohilkhand University, Bareilly, 243 006, UP, India

    Kamal Kishore

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  1. Phool Chandra
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  2. Kamal Kishore
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Correspondence to Phool Chandra.

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Chandra, P., Kishore, K. & Ghosh, A.K. Evaluation of antacid capacity and antiulcer activity of Calendula officinalis L. in experimental rats. Orient Pharm Exp Med 15, 277–285 (2015). https://doi.org/10.1007/s13596-015-0195-5

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