Abstract
Long noncoding RNAs (lncRNAs) are abnormally expressed in many malignant tumors and involved in regulating the malignant phenotypes of cancer cells. However, the role of LINC00665 in colorectal cancer (CRC) and its regulatory mechanism remain unclear. In this study, real-time polymerase chain reaction (RT-PCR) was used to detect the expressions of LINC00665, miR-9-5p and activating transcription factor 1 (ATF1) mRNA in CRC tissues. The expression of ATF1 in CRC tissues was also detected by immunohistochemistry and Western blot. CCK-8 and colony formation assays were employed to detect cell proliferation. Cell cycle and apoptosis were detected by flow cytometry analysis. Scratch healing assay and Transwell test were exploited to detect cell migration and invasion. The targeting relationships between LINC00665 and miR-9-5p, and miR-9-5p and ATF1 were validated by dual luciferase reporter assay. We found that LINC00665 was significantly overexpressed in CRC tissues, and it was also negatively correlated with the expression of miR-9-5p and positively associated with the expression of ATF1. Besides, LINC00665 promoted the proliferation, migration and invasion of CRC cells, and inhibited cell apoptosis by sponging miR-9-5p. ATF1 was proved to be the downstream target of miR-9-5p and was indirectly regulated by LINC00665. Collectively, it is concluded that LINC00665 contributes to the progression of CRC by regulating miR-9-5p/ATF1 axis.
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The data used to support the findings of this study are available from the corresponding author upon request.
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06 December 2022
This article has been retracted. Please see the Retraction Notice for more detail: https://doi.org/10.1007/s13577-022-00834-x
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This study was supported by Shanghai Committee of Science and Technology (15DZ1941304).
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Our study was approved by the ethics review board of the Yangpu Hospital (approval ID: 201806108). This study was carried out in according to the Declaration of Helsinki.
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Zhao, X., Weng, W., Long, Y. et al. RETRACTED ARTICLE: LINC00665/miR-9-5p/ATF1 is a novel axis involved in the progression of colorectal cancer. Human Cell 33, 1142–1154 (2020). https://doi.org/10.1007/s13577-020-00393-z
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DOI: https://doi.org/10.1007/s13577-020-00393-z