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Neuronal Apoptosis and Imbalance of Neurotransmitters Induced by Acetamiprid in Rats

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Abstract

Recent toxicological studies have demonstrated that exposure to organochlorine pesticides is susceptible to produce various alterations in brain cell which significantly contribute to a loss in neurobehavioral skills and disturbance of neuronal function. Acetamipride (AC) is belonging to this organochlorines family and it is considered less harmful by toxicovigilance systems and practices in Algeria. The aim of this work was mainly to evaluate the impact of this pesticide on the brain cell integrity and function in Acetamiprid-treated rats at the dose of 3.14 mg/kg (1/60 Lethal Dose) daily during 3 months. Several indicators of neuronal apoptosis and function have been rated, in addition to classical labyrinth and Maze tests monitoring to evaluate learning and memorization abilities in rats exposed to this neonicotinoid. The results of this study have shown significant enhancing of cytochrome-c(p<0.01) and Caspase-3(p<0.001) activities in brain lysates of treated group that is correlated with induction of apoptosis. At the same time, assessment of neurotrans-mitters brain cells has recorded a significant increase (p<0.01) in adrenaline rate and a significant decrease in cerebral level of dopamine (p< 0.01), serotonin (p<0.001) and Acetylcholin esterase in the same group comparing to control. Furthermore, neurobehavioral study has shown a strong correlation between the unbalance observed in neurotransmitters homeostasis and the significant (p<0.01) loss of learning, memorization and locomotive potential as demonstrated by the increase in arrival time(S) (10.33±3.14) versus (3.33±2.05) in control. In conclusion, exposition of the rats to Acetamiprid generates apoptosis which is induced by releasing of mitochondrial Cytochrome-c in cell cytosol and alters neurotransmitters rates that could reduce the potential of learning and memorization in the rats.

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Acknowledgments

First, our thanks are addressed to DGRSDT of Algeria for its support. All the labs to which the co-authors are affiliated are also thanked for authorizing our team to achieve this work.

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Authors and Affiliations

  1. Laboratory of Toxicology, University of Tebessa, 12002, Tebessa, Algeria

    Salim Gasmi & Rachid Rouabhi

  2. Faculty of Natural and Life Sciences, University of Batna2, Fesdis, Batna, 5000, Algeria

    Smail Chafaa & Mohamed Kebieche

  3. Laboratory of Molecular and Cell Biology, University of Jijel, Jijel, 18000, Algeria

    Zhora Lakroun

  4. Departement of Biology, University of Annaba, 23000, Annaba, Algeria

    Chouaib Touahria

  5. Université de Lorraine, Unité UR AFPA, EA3998, INRA USC 0340, MRCA, NAB, BP 4102, 57040 Metz, University de Lorraine, Metz, France

    Rachid Soulimani

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  1. Salim Gasmi
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  2. Smail Chafaa
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  3. Zhora Lakroun
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Correspondence to Salim Gasmi or Mohamed Kebieche.

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All applicable international, national, and/or institutional guidelines for the care and use of Rats were followed.

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Conflict of Interest

Salim Gasmi declares that he has no conflicts of interest. Smail Chafaa declares that he has no conflicts of interest. Zhora Lakroun declares that she has no conflicts of interest. Rachid Rouabhi declares that he has no conflicts of interest. Chouaib Touahria declares that he has no conflicts of interest. Mohamed Kebieche declares that he has no conflicts of interest. Rachid Soulimani declares that he has no conflicts of interest.

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Gasmi, S., Chafaa, S., Lakroun, Z. et al. Neuronal Apoptosis and Imbalance of Neurotransmitters Induced by Acetamiprid in Rats. Toxicol. Environ. Health Sci. 11, 305–311 (2019). https://doi.org/10.1007/s13530-019-0417-1

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  • DOI: https://doi.org/10.1007/s13530-019-0417-1

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