Abstract
Cypermethrin is an active pesticide that has been used for more than three decades which controls a wide variety of pests in agriculture. The present study designed to evaluate the protective role of zinc in attenuating cypermethrin induced haematological toxicity and oxidative stress in erythrocytes of male rat. Wistar male rats received oral cypermethrin at two dose levels and zinc alone or zinc pre-treatment with cypermethrin for consecutive 14 days. The toxic effects of cypermethrin were observed on various haematological parameters. Administration of cypermethrin resulted in enhanced malondialdehyde level, depletion of reduced glutathione content and antioxidant enzymes of rat erythrocytes. However pre-treatment of zinc in combination with cypermethrin improved haematological status and restored the oxidative markers to their normal levels. Based upon these data, the present study reveals that zinc treatment attenuates cypermethrin exposed haematotoxicity.
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Shafer, T. J., Meyer, D. A. & Kroften, K. M. Developmental neurotoxicity of pyrethroid insecticides: Critical review and future research needs. Environ. Hlth. Persp. 113, 123–136 (2005).
Hutson, D. H., Gaughan, L. C. & Casida, J. E. Metabolism of the cis-and trans-isomers of cypermethrin in mice. Pesticide Sci. 12, 385–398 (1981).
El-Demerdash, F. M. Lambda-cyhalothrin-induced changes in oxidative stress biomarkers in rabbit erythrocytes and alleviation effect of some antioxidants. Toxicology in Vitro. 21, 392–397 (2007).
Shah, M. K., Khan, A., Rizvi, F., Siddique, M. & Rehman, S. U. Effect of cypermethrin on clinico-haematological parameters in rabbits. Pakistan Vet. J. 27, 171–175 (2007).
Sakr, S. A., El-Mesady, F. A. & El-Desouki, N. I. Pyrethroid Inhalation Induced Histochemical Changes in the liver of Albino Rats. The Science 2, 24–28 (2002).
Jee, J. H., Masroor, F. & Kang, J. C. Responses of cypermethrin-induced stress in haematological parameters of Korean rockfish, Sebastesschlegeli. Aquac. Res. 36, 898–905 (2005).
Singh, V. K. & Saxena, P. N. Effect of cybil (cypermethrin 25EC) and cybil-sevin (carbaryl 50EC) combination on liver and serum phosphates in Wistar albino rats. J. Ecophysiol. Occup. Health. 1, 229–234 (2001).
Anwar, K. Toxic effects of cypermethrin on the development of muscle in chick embryo of Gallus domesticus. Int. J. Agricult. Biol. 6, 400–406 (2004).
Yavasoglu, A. et al. The pyrethroid cypermethrin induced biochemical and histological alterations in rat liver. J. Health Sci. 52, 774–780 (2006).
Rana, N., Saxena, N., Sharma, H. N. & Saxena, P. N. Comparative genotoxicity of alpha-cyanopyrethroids on Drosophila melanogaster. Entamon. 33, 135–138 (2008).
Jagdale, G. B., Kamoun, S. & Grewal, P. S. Entomopathogenic nematodes induce components of systemic resistance in plants: Biochemical and molecular evidence. Biological Control. 51, 102–109 (2009).
Muthuviveganandavel, V., Muthurama, P., Muthu, S. & Srikumar, K. S. Individual and combined biochemical and histological effect of cypermethrin and carbendazim in male albino rats. J. Appl. Pharmaceut. Sci. 1, 121–129 (2011).
Bhushan, B., Saxena, P. N. & Saxena, N. Biochemical and histological changes in rat liver caused by cypermethrin and beta-cyfluthrin. Arh. Hig. Rada. Toksikol. 64, 57–67 (2013).
Sangha, G. K., Kaur, K. & Khera, K. S. Cypermethrin induced pathological and biochemical changes in reproductive organs of female rats. J. Environ. Biol. 34, 99–105 (2013).
Kale, M., Rathore, N., John, S. & Bhatnagar, D. Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicology Letters 105, 197–205 (1999).
Durak, D. et al. Mercury chloride-induced oxidative stress in human erythrocytes and the effect of vitamins C and E in vitro. Afr. J. Biotechnol. 9, 488–495 (2010).
Goel, A., Danni, V. & Dhawan, D. K. Role of Zinc in mitigating toxic effects of chlorpyrifos on haematological alterations and electron microscopic observations in rat blood. BioMetals 19, 483–492 (2006).
Atessahin, A. et al. The Effects of Vitamin E and Selenium on Cypermethrin-Induced Oxidative Stress in Rats. Turk. J. Vet. Anim. Sci. 29, 385–391 (2005).
Saxena, R. & Garg, P. Vitamin E provides protection against in vitro oxidative stress due to pesticide (Chlorpyrifos and Endosulfan) in goat RBC. GERF Bulletin of Biosciences 1, 1–6 (2010).
Hymavathi, V. & Rao, L. M. Effect of sublethal concentrations of lead on the haematology and biochemical constituents of Channapunctatus. Bull. Pure Appl. Sci. 19, 1–5 (2000).
Calabrese, A. L., Thurberg, F.P., Dawson, M. A. & Wenzl, D. R. in Sublethal physiological stress induced by cadmium and mercury in winter flounder Pseudopleuronectes americanus. In sublethal effect of toxic chemicals (eds Koeman, J.H. & Strik, J.J.T.W.A.) 15-21 (Elsevier, Scientific Co. Amsterdam, 1975).
Tan, D. X., Reiter, R. J. & Chen, L. D. Both physiological and pharmacological levels of melatonin reduce DNA adduct formation induced by the carcinogen safrole. Carcinogenesis 15, 215–18 (1994).
Suganya, M., Karthi, S. & Shivakumar, M. S. Effect of Cadmium and Lead Exposure on Tissue Specific Antioxidant Response in Spodopteralitura. Free Radicals and Antioxidants 6, 90–100 (2015).
Simsek, F. et al. Oxidant and antioxidant status in beta thalassaemia major patients. J. Ankara Univ. Faculty Med. 58, 34–38 (2005).
Clemens, M. R. & Waller, H. D. Lipid peroxidation in erythrocytes. Chem. Phys. Lipids 45, 251–268 (1987).
Stern, A. Red cell oxidative damage. in Oxidative Stress (ed Sies, H.) 331-349 (Academic Press, New York, 1985).
Nasuti, C., Cantalamessa, F., Falcioni, G. & Gabbianelli, R. Different effects of type I and type II pyrethroids on erythrocyte plasma membrane properties and enzymatic activity in rats. Toxicology 191, 233–244 (2003).
Prasanthi, K. Muralidhara & Rajini, P. S. Morphological and biochemical perturbations in rat erythrocytes following in vitro exposure to fenvalerate and its metabolite. Toxicology in Vitro 19, 449–456 (2005).
Xiu, Y. M. Trace elements in health and diseases. Biomed. Environ. Sci. 9, 130–136 (1996).
Kang, Y. J. & Zhou, Z. Zinc prevention and treatment of alcoholic liver disease. Mol. Aspects. Med. 26, 391–404 (2005).
Zhou, Z. et al. Zinc supplementation prevents alcoholic liver injury in mice through attenuation of oxidative stress. Am. J. Pathol. 166, 1681–1690 (2005).
Prasad, A. S. Zinc in human health: effect of zinc on immune cells. Mol. Med. 14, 353 (2008).
Cagen, S. Z. & Klaassen, C. D. Protection of carbon tetrachloride-induced hepatotoxicity by zinc: role of metallothionein. Toxicol. Appl. Pharmacol. 51, 107–116 (1979).
Cabre, M. et al. Inhibition of hepatic cell nuclear DNA fragmentation by zinc in carbon tetrachloride treated rats. J. Hepatol. 31, 228–234 (1999).
Prasad, A. S. Clinical, immunological, anti-inflammatory and antioxidant roles of zinc. Exp Gerontology 43, 370–377 (2008).
Kazi, T. G. et al. Effects of Mineral Supplementation on Liver Cirrhotic/Cancer Male Patients. Biol. Trace Elem. Res. 150, 81–90 (2012).
Mandal, A., Chakraborty, S. & Lahiri, P. Hematological changes produced by lindane (-HCH) in six species of birds. Toxicology 40, 103–111 (1986).
Shakoori, A. R., Aslam, F. & Sabir, M. Effect of prolonged administration of insecticide (cyhalothrin/karate) on the blood and liver of rabbits. Folia Biol. 40, 91–99 (1992).
Morgan, D. P., Stockdale, E. M., Roberts, R. J. & Walter, H. W. Anaemia associated with exposure to’ lindane. Arc. Environ. Hlth. 35, 307–310 (1980).
Rahman, M. F., Siddiqui, M. K. J., Mahaboob, M. & Mustafa, M. J. Hematological and hepatotoxic effects of isoprocrab in chicken. J. Appl. Toxicol. 10, 187–92 (1990).
Yousef, M. I., El-Deerdash, F. M., Kamel, K. I. & Al-Salhen, K. S. Changes in some haematological and biochemical indices of rabbits induced by isoflavones and cypermethrin. Toxicol. 189, 223–234 (2003).
Latimer, K. S., Mahaffey, E. A. & Prasse, K. W. in Clinical Pathology. Veterinary Laboratory Medicine. 4th Edn. (Iowa State Univ. Press, USA., 2004).
Kobayashi, S. D., Voyich, J. M. & DeLeo, F. R. Regulation of the neutrophil-mediated inflammatory response to infection. Microbes Infect. 5, 1337–1344 (2003).
Goel, A., Dani, V. & Dhawan, D. K. Protective effects of zinc on lipd peroxidation, antioxidant enzymes and hepatic histo architecture in chlorpyrifos-induced toxicity. Chem. Biol Interact. 156, 131–140 (2005).
Prasad, A. S. Clinical, biochemical and nutritional spectrum of zinc deficiency in human subjects: an update. Nutr. Rev. 41, 197–208 (1983).
Commercial and Experimental Organic Insecticides, http://aesa.oxfordjournals.org/content/54/4/518 (1985).
Barger, A. M. The complete blood cell count: a powerful diagnostic tool. Vet. Clin. Small Anim. 33, 1207–22 (2003).
Schara, M. et al. The action of mercury on cell membranes. Cell Mol. Biol. Lett. 6, 299–304 (2001).
Brandão, R. et al. Hemolytic effects of sodium selenite and mercuric chloride in human blood. Drug Chem. Toxicol. 28, 397–407 (2005).
Wang, Y., Fang, J., Leonard, S. S. & Rao, K. M. Cadmium inhibits the electron transfer chain and induces reactive oxygen species. Free Radic. Biol. Med. 36, 1434–1443 (2004).
Panda, S., Gupta, P. & Kar, A. Protective role of ashwagandha in cadmium-induced hepatotoxicity and nephrotoxicity in male mouse. Curr. Sci. 72, 546–547 (1997).
Sandrini, J. Z. et al. Short term responses to cadmium exposure in the estuarine polychaete Laeonereisacuta (Polychaeta, Nereididae): subcellular distribution and oxidative stress generation. Environ. Toxicol. Chem. 25, 1337–1344 (2006).
Environmental Health Criteria 95 (1990).
Banerjee, B. D. et al. Biochemical effectsof some pesticides on lipid peroxidation and freeradical scavengers. Toxicol. Lett. 107, 33–47 (1999).
Gultekin, F., Delibas, N., Yasar, S. & Kilinc, I. In vivo changes in antioxidant systems andprotective role of melatonin and a combinationof vitamin C and vitamin E on oxidative damagein erythrocytes induced by chlorpyrifos-ethyl in rats. Arch. Toxicol. 75, 88–96 (2001).
Goel, A., Dani, V. & Dhawan, D. K. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture inchlorpyrifos induced toxicity. Chem. Biol. Interact. 156, 131–140 (2005).
Chvapil, M., Ryan, J. N. & Zukoski, C. F. Effect of zinc on lipid peroxidation in liver microsomes and mitochondria. Proc. Soc. Exp. Biol. Med. 141, 150–153 (1972).
Nicotera, P. & Orrenius, S. Role of thiols in protection against biological reactive intermediates. Adv. Exp. Med. Biol. 197, 41–49 (1986).
Schaffer, S., Azuma, J., Takahashi, K. & Mozaffari, M. Why is taurine cytoprotective? Adv. Exp. Med. Biol. 526, 307–321 (2003).
Flohe, B. R. Tissue specific function of individual glutathione peroxidases. Free Radic. Biomed. 27, 951–965 (1999).
Kyle, M. E., Miccadei, S., Nakae, D. & Farber, J. L. Superoxide dismutase and catalase protect cultured hepatocytes from the cytotoxicity of acetaminophen. Biochem. Biophys. Res. Commun. 149, 889–896 (1987).
Jalaili, S., Farshid, A. A. & Heydari, R. Histopathological observation on protective effect of vitamin E on endosulfan induced cardiotoxicity in rat. Pak. J. Biol. Sci. 10, 1922–1925 (2007).
Hayes, J. D. & Pulford, D. The glutathione-S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit. Rev. Biochem. Mol. Biol. 30, 445–600 (1995).
Erdem, A. et al. The protective effect of taurine against gentamicin-induced acute tubular necrosis in rats. Nephrol. Dial. Transplant. 15, 1175–1182 (2000).
Deisseroth, A. & Dounce, A. L. Catalase: Physical and chemical properties, mechanism of catalase, and physiological role. Phsiol. Rev. 50, 319–375 (1970).
Jacob, R. A. The integrated antioxidant system. Nutr. Res. 15, 755–766 (1995).
Skhra, J., Hodinar, A., Kvosnicka, J. & Hilgertova, J. Relationship of oxidative stress and fibrinolysis in diabetes mellitus. Diab. Med. 13, 800–805 (1996).
FAO specifications and evaluations for agricultural pesticides (Food and Agriculture Organization of the United Nations, http://www.fao.org/fileadmin/templates/ agphome/documents/Pests_Pesticides/Specs/ chlors04.pdf (2006).
Wintrobe, M. M. in Clinical hematology 6th Edn (Lea and Febiger, USA, 1967).
Dacie, J. V. & Lewis, S. M. in Practical Haematology 5th. Edn (Churchill Livingstone, London, 1975).
Mgbenka, B. O., Oluah, N. S. & Umeike, I. Effect of Gammalin 20 (Linndane) on differential white blood cells count of the African catfish Clariasalbo punctatus. Bull. Environ. Contam. Toxicol. 71, 248–254 (2003).
Benjamin, M. M. in Outline of Veterinary Clinical Pathology 3rd Edn (University Press, USA, 1978).
Becton-Dickinson. Unopette WBC/Platelet determination for manual methods. Rutherford, N.J.: Becton, Dickinson, and Company (1996).
Ohkawa, H., Onishi, N. & Yagi, K. Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95, 351–358 (1979).
Griffith, O. W. Glutathione turnover in human erythrocytes. J. Biochem. 256, 4900–4904 (1981).
Griffith, O. W. Determination of glutathione and glutathione disulphide using glutathione reductase and 2-vinylpyridine. Anal. Biochem. 106, 207–212 (1980).
Rotruck, J. T. et al. Selenium: Biochemical role as a component of glutathione peroxidise. Sci. 179, 588–590 (1973).
Habig, W. H., Pabst, M. J. & Jakoby, W. B. Glutathione-S-Transferase: the first enzymatic step in mercapturic acid formation. J. Biolchem. 249, 7130–7139 (1974).
Aebi, H. Catalase. in Method of enzymetic analysis (ed Bergmeyer, H.U.) 674-684 (Academic Press, NewYork, 1974).
Marklund, S. & Marklund, G. Involvement of superoxide anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469–474 (1974).
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Das, T., Pradhan, A., Paramanik, A. et al. Ameliorative role of zinc on cypermethrin-induced changes in haematological parameters and oxidative stress biomarkers in rat erythrocytes. Toxicol. Environ. Health Sci. 8, 234–246 (2016). https://doi.org/10.1007/s13530-016-0280-2
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DOI: https://doi.org/10.1007/s13530-016-0280-2