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Effects of green-synthesized CuO and ZnO nanoparticles on ruminal mitigation of methane emission to the enhancement of the cleaner environment

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Abstract

This study was carried out to determine the effects of adding CuO and ZnO nanoparticles (at levels of 0, 10, and 20 mg of CuO and ZnO nanoparticles in mL rumen fluid) on in vitro gas and methane production of alfalfa (Medicago sativa) hay. Green-synthesized CuO and ZnO nanoparticles were characterized using scanning electron microscope (SEM), Fourier transform infrared (FTIR), energy dispersive X-Ray spectroscopy (EDX), and atomic force microscopy (AFM) microscopes. The use of CuO and ZnO nanoparticles had significant effects on ruminal protozoa colonization, in vitro gas production, and mitigation of methane emission (P < 0.05). The use of CuO nanoparticles led to a decrease in the protozoa population, thus indirectly decreasing the ruminal fermentation. The CuO nanoparticle is not recommended biologically, but ZnO increased protozoa population and gas production; therefore, this nanoparticle could only exert beneficial effects on rumen fermentation and is recommended.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

CuO:

Copper (II) oxide

ZnO:

Zinc oxide

SEM:

Scanning electron microscope

FTIR:

Fourier transform infrared

EDX:

Energy dispersive X-ray spectroscopy

AFM:

Atomic force microscopy

VFA:

Volatile fatty acids

NAD+ :

Nicotinamide adenine dinucleotide

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Acknowledgements

The experiment was supported by Atatürk-BAP under the project number of FOA-2020-7399. The authors wish to express their sincere gratitude to Dr. Hamid Paya, Maghsoud Besharati, and Selim Esen for their valuable cooperation in technical assistance.

Funding

This research was supported by the grants number FOA-2020–7399 from Atatürk University.

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Authors and Affiliations

  1. Department of Animal Science, Agricultural Faculty, Ataturk University, 25240, Erzurum, Turkey

    Valiollah Palangi & Muhlis Macit

  2. Department of Food Technology, Erzurum Vocational College, Ataturk University, 25240, Erzurum, Turkey

    Hayrunnisa Nadaroglu

  3. Department of Nano-Science and Nano-Engineering, Institute of Science and Technology, Ataturk University, 25240, Erzurum, Turkey

    Hayrunnisa Nadaroglu

  4. Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, 51666, Iran

    Akbar Taghizadeh

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  1. Valiollah Palangi
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  2. Muhlis Macit
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  4. Akbar Taghizadeh
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Contributions

VP initiated the project idea. VP and MM contributed to the idea, design, and execution of the study. HN and AT were in charge of laboratory analyses. VP collected and analyzed the data. VP drafted and finalized the manuscript.

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Correspondence to Valiollah Palangi.

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Palangi, V., Macit, M., Nadaroglu, H. et al. Effects of green-synthesized CuO and ZnO nanoparticles on ruminal mitigation of methane emission to the enhancement of the cleaner environment. Biomass Conv. Bioref. 14, 5447–5455 (2024). https://doi.org/10.1007/s13399-022-02775-9

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  • DOI: https://doi.org/10.1007/s13399-022-02775-9

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