Abstract
A new biogenic chitosan biopolymer-functionalized zinc-doped bismuth oxide nanoneedle was successfully synthesized by an ultrasound-assisted chemical precipitation method that annealed at 250°C. In this process, an appropriate amount of chitosan, Bi (NO3)2, sodium hydroxide and varying molar ratios of Zn (NO3)2 were used as the source materials. The obtained materials were subjected to annealing at 250°C and then characterized with X-ray diffraction, Fourier transform infrared spectroscopy, high-resolution scanning electron microscope, transmittance electron microscope, and UV–Vis–NIR spectroscopy (UV–Vis–NIR). The electrochemical properties of the materials were investigated by electrochemical impedance spectroscopy, and cyclic voltammetry in the pH range is 4 with (0.1) M buffer solution. Finally, the potential toxicity and antibacterial activity of the materials were investigated against Staphylococcus aureus, Escherichia coli and cancer cell lines HepG2 and C3A. The result indicates that CS/Zn0.75Bi2O3 nanoneedle shows zones of inhibition against S. aureus as 32 mm and E. coli as 35 mm. In the anticancer analysis, CS/Zn0.75Bi2O3 nanoneedles showed a maximum cell inhibition of 65.45% at 100 μg/mL−1.
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Ratova, M.; Redfern, J.; Verran, J.; Kelly, P.J.: Highly efficient photocatalytic bismuth oxide coatings and their antimicrobial properties under visible light irradiation. Appl. Catal. B Environ. 239(1), 223–232 (2018)
Song, Y.; Jiang, H.; Bi, H.; Zhong, G.; Chen, J.; Wu, Y.; Wei, W.: Multifunctional bismuth oxychloride/mesoporous silica composites for photocatalysis, antibacterial test, and simultaneous stripping analysis of heavy metals. ACS Omega 3(1), 973–981 (2018)
Iyyapushpam, S.; Nishanthi, S.T.; Pathinettam Padiyan, D.: Synthesis of room temperature bismuth oxide and its photocatalytic activity. Mater. Lett. 86(1), 25–27 (2012)
Wang, H.W.; Hu, Z.A.; Chang, Y.Q.; Chen, Y.L.; Lei, Z.Q.; Zhang, Z.Y.; Yang, Y.Y.: Facile solvothermal synthesis of a graphene nanosheet–bismuth oxide composite and its electrochemical characteristics. Electrochim. Acta 55(28), 8974–8980 (2010)
Periasamy, A.P.; Yang, S.; Chen, S.M.: Preparation and characterization of bismuth oxide nanoparticles-multiwalledcarbon nanotube composite for the development of horseradish peroxidase based H2O2 biosensor. Talanta 87(15), 15–23 (2011)
Diaz-Guerra, C.; Almodovar, P.; Camacho-Lopez, M.; Camacho-Lopez, S.; Piqueras, J.: Formation of β-Bi2O3 and δ-Bi2O3 during laser irradiation of Bi films studied in situ by spatially resolved Raman spectroscopy. J. Alloys Compd. 723(5), 520–526 (2017)
Yavo, N.; Smith, A.D.; Yeheskel, O.; Cohen, S.R.; Korobko, R.; Wachtel, E.; Slater, P.R.; Lubomirsky, I.: Large nonclassical electrostriction in (Y, Nb)-stabilized δ-Bi2O3. Adv. Funct. Mater. 26(7), 1138–1142 (2016)
Lim, H.; Rawal, S.B.: Integrated Bi2O3 nanostructure modified with Au nanoparticles for enhanced photocatalytic activity under visible light irradiation. Prog. Nat. Sci. Mater. Int. 27(3), 289–296 (2017)
Elumalai, K.; Velmurugan, S.; Ravi, S.; Kathiravan, V.; Adaikala Raj, G.: Bio-approach: Plant mediated synthesis of ZnO nanoparticles and their catalytic reduction of methylene blue. Adv. Powder Technol. 26(6), 1639–1651 (2015)
Shi, L.E.; Li, Z.H.; Zheng, W.; Zhao, Y.F.; Jin, Y.F.; Tang, Z.X.: Synthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: a review. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess 31(2), 173–186 (2014)
Sirelkhatim, A.; Mahmud, S.; Seeni, A.; Mohamad Kaus, N.H.; Chuo Ann, L.; Mohd Bakhori, S.K.; Hasan, H.; Mohamad, D.: Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano Micro Lett. 7(3), 219–242 (2015)
Lakshmi Prasanna, V.; Vijayaraghavan, R.: Insight into the mechanism of antibacterial activity of ZnO: surface defects mediated reactive oxygen species even in the dark. Langmuir 31(33), 9155–9162 (2015)
Cornei, N.; Tancret, N.; Abraham, F.; Mentré, O.: New ε-Bi2O3 meta stable polymorph. Inorg. Chem. 45(13), 4886–4888 (2006)
Demirel, R.; Suvac, E.; Şahin, İ.; Dag, S.; Kilic, V.: Antimicrobial activity of designed undoped and doped MicNo–ZnO particles. J. Drug Deliv. Sci. Technol. 47(1), 309–321 (2018)
Kaviyarasu, K.; Maria Magdalane, C.; Jayakumar, D.; Samson, Y.; Bashir, A.K.H.; Maaza, M.; Letsholathebe, D.; Mahmoud, A.H.; Kennedy, J.: High performance of pyrochlore like Sm2Ti2O7 heterojunction photocatalyst for efficient degradation of rhodamine-B dye with waste water under visible light irradiation. J. King Saud Univ. Sci. 32(2), 1516–1522 (2020)
Kaviyarasu, K.; Mola, G.T.; Oseni, S.O.; Kanimozhi, K.; Maria Magdalane, C.; Kennedy, J.; Maaza, M.: ZnO doped single wall carbon nanotube as an active medium for gas sensor and solar absorber. J. Mater. Sci. Mater. Electron. 30, 147–158 (2019)
Prakash, T.; Williams, G.V.M.; Kennedy, J.; Rubanov, S.: High spin-dependent tunneling magneto resistance in magnetite powders made by arc discharge. J. Appl. Phys. 120, 123905 (2016)
Raja, A.; Rajasekaran, P.; Selvakumar, K.; Arunpandian, M.; Kaviyarasu, K.; Asath Bahadur, S.; Swaminathan, M.: Visible active reduced graphene oxide-BiVO4-ZnO ternary photocatalyst for efficient removal of ciprofloxacin. Sep. Purif. Technol. 233, 115996 (2020)
Rathnakumar, S.M.; Noluthando, K.; Kulandaiswamy, A.J.; Rayappan, J.B.B.; Kasinathan, K.; Kennedy, J.; Maaza, M.: Stalling behaviour of chloride ions: a non-enzymatic electrochemical detection of α-Endosulfan using CuO interface. Sens. Actuators B 293, 100–106 (2019)
Maria Magdalane, C.; Kaviyarasu, K.; Maria Assuntha Priyadharsini, G.; Bashir, A.K.H.; Mayedwa, N.; Matinise, N.; Isaeve, A.B.; Abdullah Al-Dhabif, N.; Arasuf, M.V.; Arokiyaraj, S.; Kennedy, J.; Maaza, M.: Improved photocatalytic decomposition of aqueous Rhodamine-B by solar light illuminated hierarchical yttria nanosphere decorated ceria nanorods. J. Mater. Res. Technol. 8(3), 2898–2909 (2019)
Kaviyarasu, K.; Maria Magdalane, C.; Kanimozhi, K.; Kennedy, J.; Siddhardha, B.; Subba Reddy, E.; Rotte, N.K.; Sharma, C.S.; Thema, F.T.; Letsholathebe, D.; Tessema Mola, G.; Maaza, M.: Elucidation of photocatalysis, photoluminescence and antibacterial studies of ZnO thin films by spin coating method. J. Photochem. Photobiol. B 173, 466–475 (2017)
Zhang, L.; Sun, F.; Zuo, Y.; Fan, C.; Xu, S.; Yang, S.; Gu, F.: Immobilisation of CdS nanoparticles on chitosan microspheres via a photochemical method with enhanced photocatalytic activity in the decolourisation of methyl orange. Appl. Catal. B 156-157(1), 293–300 (2014)
Farzana, M.H.; Meenakshi, S.: Removal of acid blue 158 from aqueous media by adsorption onto cross-linked chitosan beads. J. Chitin Chitosan Sci. 1(1), 50–58 (2013)
Elanchezhiyan, S.S.; Sivasurian, N.; Meenakshi, S.: Recovery of oil from oil-in-water emulsion using biopolymers by adsorptive method. Int. J. Biol. Macromol. 70(1), 399–407 (2014)
Sowmya, A.; Meenakshi, S.: Zr (IV) loaded cross-linked chitosan beads with enhanced surface area for the removal of nitrate and phosphate. Int. J. Biol. Macromol. 69(1), 336–343 (2014)
Jiang, M.; Liu, X.; Wang, H.: Conductive and transparent Bi-doped ZnO thin films prepared by rf magnetron sputtering. Surf. Coat. Technol. 203(24), 3750–3753 (2009)
Chandraboss, V.L.; Natanapatham, L.; Karthikeyan, B.; Kamalakkannan, J.; Prabha, S.; Senthilvelan, S.: Effect of Bismuth doping on the ZnO nanocomposite material and study of its photocatalytic activity under UV-light. Mater. Res. Bull. 40(10), 3707–3712 (2013)
Sun, A.; Chen, H.; Song, C.; Jiang, F.; Wang, X.; Fu, Y.: Magnetic Bi25FeO40-graphene catalyst and its high visible-light photocatalytic performance. RSC Adv. 3(13), 4332–4340 (2013)
Jabeen Fatima, M.J.; Navaneeth, A.; Sindhu, S.: Improved carrier mobility and bandgap tuning of zinc doped bismuth oxide. RSC Adv. 5(4), 2504–2510 (2015)
Zhong, S.; Zou, S.; Peng, X.; Ma, J.; Zhang, F.: Effects of calcination temperature on preparation and properties of europium-doped bismuth oxide as visible light catalyst. J. Sol–Gel. Sci. Technol. 74(1), 220–226 (2015)
Mastan, R.; Khorsand, Zak A.; Pilevar Shahri, R.: Bi-doped ZnO yellow nanopigments: synthesis, characterization, and antibacterial application for painting humid places. Ceram. Int. 46(7), 8582–8587 (2020)
Molloy, M.P.; Herbert, B.R.; Slade, M.B.; Rabilloud, T.; Nouwens, A.S.; Williams, K.L.; Gooley, A.A.: Proteomic analysis of the Escherichia coli outer membrane. Eur. J. Biochem. 267(10), 2871–2881 (2000)
Yin, Y.; Dang, Q.; Liu, C.; Yan, J.; Cha, D.; Yu, Z.; Cao, Y.; Wang, Y.; Fan, B.: Itaconic acid grafted carboxymethyl chitosan and its nanoparticles: preparation, characterization and evaluation. Int. J. Biol. Macromol. 102(1), 10–18 (2017)
Zavareh, S.; Behrouzi, Z.; Avanes, A.: Cu(II) binded chitosan/Fe3O4 nanocomomposite as a new biosorbentfor efficient and selective removal of phosphate. Int. J. Biol. Macromol. 101(1), 40–50 (2017)
Kannusamy, P.; Sivalingam, T.: Chitosan-ZnO/polyaniline hybrid composites: polymerization of aniline with chitosan-ZnO for better thermal and electrical property. Polym. Degrad. Stab. 98(5), 2988–2996 (2013)
Tajally, M.; Mirzaee, O.; Eshaghi, A.: The effects of Ti concentration on the structure, optical, and electrical properties of Al and Ti co-doped ZnO thin films. Optik 127(11), 4645–4649 (2016)
Osman, Z.; Arof, A.K.: FTIR studies of chitosan acetate based polymer electrolytes. Electrochim. Acta 48(8), 993–999 (2003)
Pawlak, A.; Mucha, M.: Thermogravimetric and FTIR studies of chitosan blends. Thermochim. Acta 396(1–2), 153–166 (2003)
Rao, K.S.V.K.; Reddy, P.R.; Lee, Y.I.; Kim, C.: Synthesis and characterization of chitosan–PEG–Ag nanocomposites for antimicrobial application. Carbohydr. Polym. 87(1), 920–925 (2012)
Abdelwahab, N.A.; Helaly, F.M.: Simulated visible light photocatalytic degradation of Congo red by TiO2 coated magnetic polyacrylamide grafted carboxy methylated chitosan. J. Ind. Eng. Chem. 50(1), 162–171 (2017)
Dudhani, A.R.; Kosaraju, S.L.: Bioadhesive chitosan nanoparticles: preparation and characterization. Carbohydr. Polym. 81(2), 243–251 (2010)
Deshpande, P.; Dapkekar, A.; Oak, M.D.; Paknikar, K.M.; Rajwade, J.M.: Zinc complexed chitosan/TPP nanoparticles: a promising micronutrient nanocarrier suited for foliar application. Carbohydr. Polym. 165(1), 394–401 (2017)
Dar, G.N.; Umar, A.; Zaidi, S.A.; Ibrahim, A.A.; Abaker, M.; Baskoutas, S.; Al- Assiri, M.S.: Ce-doped ZnO nanorods for the detection of hazardous chemical. Sens. Actuators B 173(1), 72–78 (2012)
Naji Aljawf, R.; Rahman, F.; Kumar, S.: Defects/vacancies engineering and ferromagnetic behavior in pure ZnO and ZnO doped with Co nanoparticles. Mater. Res. Bull. 83(1), 108–115 (2016)
Ai, Z.; Huang, Y.; Lee, S.; Zhang, L.: Monoclinic-Bi2O3 photocatalyst for efficient removal of gaseous NO and HCHO under visible light irradiation. J. Alloys Compd. 509(5), 2044–2049 (2011)
Lv, T.; Pan, L.; Liu, X.; Lu, T.; Zhu, G.; Sun, Z.; Sun, C.Q.: One-step synthesis of CdS–TiO2–chemically reduced graphene oxide composites via microwave-assisted reaction for visible-light photocatalytic degradation of methyl orange. Catal. Sci. Technol. 2(1), 754–758 (2012)
Zeng, P.; Zhang, Q.; Peng, T.; Zhang, X.: One-pot synthesis of reduced graphene oxide–cadmium sulfide nanocomposite and its photocatalytic hydrogen production. PCCP 13(48), 21496–21502 (2011)
Hormigos, R.M.; Gismera, M.J.; TeresaSevilla, M.: Straight forward ultrasound-assisted synthesis of bismuth oxide particles with enhanced performance for electrochemical sensors development. Mater. Lett. 158(1), 359–362 (2015)
Aytimur, A.; Sinan Temel, S.K.; Uslu, B.: Boron undoped and doped europium-bismuth oxide nanocomposites via the polymeric precursor technique. J. Miner. Met. Mater. Soc. 66(1), 1479–1484 (2014)
Pawar, R.C.; Lee, C.S.: Single-step sensitization of reduced graphene oxide sheets and CdSnanoparticles on ZnO nanorods as visible-light photocatalysts. Appl. Catal. B Environ. 144(1), 57–65 (2014)
Karthik, R.; Thambidurai, S.: Synthesis of cobalt doped ZnO/reduced graphene oxide nanorods as active material for heavy metal ions sensor and antibacterial activity. J. Alloys Compd. 715(1), 254–265 (2017)
Higuchi, T.; Liu, Y.S.; Yao, P.; Glans, P.A.; Guo, J.H.; Chang, C.L.; Wu, Z.Y.; Sakamoto, W.; Itoh, N.; Shimura, T.; Yogo, T.; Hattori, T.: Electronic structure of multiferroic BiFeO3 by resonant soft x-ray emission spectroscopy. Phys. Rev. B Condens. Matter. Mater. Phys. 78(8), 085106 (2008)
Li, Z.; Shen, Y.; Guan, Y.; Hu, Y.; Lin, Y.; Nan, C.W.: Bandgap engineering and enhanced interface coupling of graphene–BiFeO3 nanocomposites as efficient photocatalysts under visible light. J. Mater. Chem. A 2(6), 1967–1973 (2014)
Ciszewski, M.; Mianowski, A.; Szatkowski, P.; Nawrat, G.; Adamek, J.: Reduced graphene oxide–bismuth oxide composite as electrode material for supercapacitors. Ionics 21(2), 557–563 (2015)
Chandraboss, V.L.; Natanapatham, L.; Karthikeyan, B.; Kamalakkannan, J.; Prabha, S.; Senthilvelan, S.: Effect of Bismuth doping on the ZnO nanocomposite material and study of its photocatalytic activity under UV-light. Mater. Res. Bull. 48(10), 3707–3712 (2013)
Han, W.; Ren, L.; Gong, L.; Qi, X.; Liu, Y.; Yang, L.; Wei, X.; Zhong, J.: Self-assembled three-dimensional graphene-based aerogel with embedded multifarious functional nanoparticles and its excellent photoelectrochemical activities. ACS Sustain. Chem. Eng. 2(4), 741–748 (2014)
Kasi, G.; Seo, J.: Influence of Mg doping on the structural, morphological, optical, thermal, and visible-light responsive antibacterial properties of ZnO nanoparticles synthesized via co-precipitation. Mater. Sci. Eng. C 98(1), 717–725 (2019)
Fang, S.W.; Li, C.F.; Shih, D.Y.C.: Antifungal activity of chitosan and its preservative effect on low-sugar candied kumquat. J. Food Prot. 57(2), 136–140 (1994)
Goy, R.C.; de Britto, D.; Assis, O.B.G.: A review of the antimicrobial activity of chitosan. Polímer 19(3), 241–247 (2009)
Wang, X.H.; Du, Y.M.; Liu, H.: Preparation, characterization and antimicrobial activity of chitosan-Zn complex. Carbohydr. Polym. 56(1), 21–26 (2004)
Inoue, Y.; Kanzaki, Y.: The mechanism of antibacterial activity of silver-loaded zeolite. J. Inorg. Biochem. 67(1–4), 377 (1997)
Bacchi, A.; Carcelli, M.; Pelagatti, P.; Pelizzi, C.; Pelizzi, G.; Zani, F.: Antimicrobialand mutagenic activity of some carbono- and thiocarbonohydrazone ligandsand their copper (II), iron (II) and zinc (II) complexes. J. Inorg. Biochem. 75(2), 123–133 (1999)
Yang, Z.H.; Xie, C.S.; Xia, X.P.; Cai, S.Z.: Zn2+ release behavior and surface characteristics of Zn/LDPE nanocomposites and ZnO/LDPE nanocomposites in simulateduterine solution. J. Mater. Sci. Mater. Med. 19(11), 3319–3326 (2008)
Azevedo, E.P.; Saldanha, T.D.P.; Navarro, M.V.M.; Medeiros, A.C.; Ginani, M.F.; Raffin, F.N.: Mechanical properties and release studies of chitosan films impregnatedwith silver sulfadiazine. J. Appl. Polym. Sci. 102(4), 3462–3470 (2006)
Qin, Y.M.; Zhu, C.J.; Chen, J.; Chen, Y.Z.; Zhang, C.: The absorption and release of silver and zinc ions by chitosan fibers. J. Appl. Polym. Sci. 101(1), 766–771 (2006)
Feng, Q.L.; Wu, J.; Chen, G.Q.; Cui, F.Z.; Kim, T.N.; Kim, J.O.: A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J. Biomed. Mater. Res. 52(4), 662–668 (2000)
Acknowledgements
I acknowledge my sincere gratitude to Dr. R. Swaminathan, Principal, Vidhyaa Giri College of Arts and Science-Puduvayal-Sivagangai for an encourage the research work. This study was supported by the National Research Foundation of Korea, which is funded by the Korean Government [NRF-2018-R1A6A1A-03024314].
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Ramaiah Karthik, first author, was involved in conceptualization, methodology, investigation, writing—original draft and formal analysis. Kannuchamy Pandiselvi performed validation, resources, writing—reviewing and editing. Karuppusamy Mariyappan contributed to methodology, formal analysis, validation, resources, writing–reviewing and editing. Jayachandran sivakamavalli, corresponding author, performed validation, visualization and supervision.
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Karthik, R., Pandiselvi, K., Mariyappan, K. et al. Synthesis of Biogenic Chitosan Biopolymer-Functionalized Zinc-Doped Bi2O3 Nanoneedles and Its Bio-applications: In Vitro Antibacterial and Anticancer activity. Arab J Sci Eng 46, 5605–5618 (2021). https://doi.org/10.1007/s13369-020-05099-w
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DOI: https://doi.org/10.1007/s13369-020-05099-w