Abstract
Rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) is an introduced phloem feeder of guava. Gut symbiotic bacteria alter the physiology of the host insect. The present study characterized the nine bacterial isolates from the second nymphal stage of rugose spiralling whitefly using seven different bacteria growth media viz., Nutrient Agar (NA), Luria Bertani agar (LBA), MacConkey agar (MCA), Tryptic Soya Agar (TSA), Endo Agar (EA), R2A agar and MRS agar. Among the nine bacterial isolates, chitinase-producing isolates (seven species) were proceeded for biochemical tests, protease and siderophore activities. Out of seven chitinase producers, protease (seven species) and siderophore (two species) producers were identified using skim milk agar and Chromazurol S (CAS) agar plates, respectively. The biochemical tests showed that all bacterial isolates were Gram positive. Bacterial isolates SGRSW2, SGRSW4, SGRSW6 and SGRSW8 showed negative for carbohydrate fermentation, indole production, citrate utilization, starch hydrolysis tests and also, SGRSW4 showed positive for lactose fermentation. 16S rRNA gene sequencing results revealed that Bacillus genus alone is associated with rugose spiralling whitefly which includes Bacillus albus, Bacillus tequilensis and Bacillus altitudinis. Bacterial communities associated with rugose spiralling whitefly offers basic knowledge about their functional significance to study the physiological role in the host insect.
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References
Ateyyat MA, Shatnawi M, Al-Mazra’awi M (2010) Isolation & identification of culturable forms of bacteria from the sweet potato whitefly Bemesia tabaci Genn. (Homoptera: Aleyrodidae) in Jordan. Turk J Agric For 34:225–234. https://doi.org/10.3906/tar-0902-35
Cappuccino JC, Sherman N (1992) Microbiology: a laboratory manual, 3rd edn. Benjamin/cummings Pub. Co., New York, pp 125–179
Cattelan AJ, Hartel PG, Fuhrmann JJ (1999) Screening for plant growth–promoting rhizobacteria to promote early soybean growth. Soil Sci Soc Am J 63:1670–1680. https://doi.org/10.2136/sssaj1999.6361670x
Ciche TA, Blackburn M, Carney JR, Ensign JC (2003) Photobactin: a catechol siderophore produced by Photorhabdus luminescens, an entomopathogen mutually associated with Heterorhabditis bacteriophora NC1 nematodes. Appl Environ Microbiol 69:4706–4713. https://doi.org/10.1128/AEM.69.8.4706-4713.2003
Davidson EW, Rosell RC, Hendrix DL (2000) Culturable bacteria associated with the whitefly, Bemisia argentifolii (Homoptera: Aleyrodidae). Fla Entomol 1:159. https://doi.org/10.2307/3496151
de Vries EJ, Breeuwer JA, Jacobs G, Mollema C (2001) The association of western flower thrips, Frankliniella occidentalis, with a near Erwinia species gut bacteria: transient or permanent? J Invertebr Pathol 77:120–128. https://doi.org/10.1006/jipa.2001.5009
Dillon RJ, Dillon VM (2004) The gut bacteria of insects: nonpathogenic interactions. Annu Rev Entomol 49:1–92. https://doi.org/10.1146/annurev.ento.49.061802.123416
Elango K, Nelson SJ (2020) Effect of host plants on the behaviour of rugose spiralling whitefly (Aleurodicus rugioperculatus) and their natural enemies. Res J Agric Sci 11:120–123
Engel P, Moran NA (2013) The gut microbiota of insects–diversity in structure and function. FEMS Microbiol Rev 37:699–735. https://doi.org/10.1111/1574-6976.12025
Genta FA, Dillon RJ, Terra WR, Ferreira C (2006) Potential role for gut microbiota in cell wall digestion and glucoside detoxification in Tenebrio molitor larvae. J Insect Physiol 52:593–601. https://doi.org/10.1016/j.jinsphys.2006.02.007
Indiragandhi P, Anandham R, Madhaiyan M, Poonguzhali S, Kim GH, Saravanan VS, Sa T (2007) Cultivable bacteria associated with larval gut of prothiofos-resistant, prothiofos-susceptible and field-caught populations of diamondback moth, Plutella xylostella and their potential for, antagonism towards entomopathogenic fungi and host insect nutrition. J Appl Microbiol 103:2664–2675. https://doi.org/10.1111/j.1365-2672.2007.03506.x
Indiragandhi P, Yoon C, Yang JO, Cho S, Sa TM, Kim GH (2010) Microbial communities in the developmental stages of B and Q biotypes of sweet potato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). J Korean Soc Appl Bl 53:605–617. https://doi.org/10.3839/jksabc.2010.093
Krishnamoorthy R, Arul Jose P, Janahiraman V, Indira Gandhi P, Gandhi Gracy R, Jalali SK, Senthil Kumar M, Malathi V, Anandham R (2020) Function and insecticidal activity of bacteria associated with papaya mealybug, Paracoccus marginatus Williams & Granara de Willink (Hemiptera: Pseudococcidae). Biocontrol Sci Technol 30:762–778. https://doi.org/10.1080/09583157.2020.1765983
Kumar V, McKenzie CL, Mannion C, Stocks I, Smith T, Osborne LS (2013) Rugose spiraling whitefly Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae). EENY578. University of Florida, IFAS Extension. Accessed 31 Dec. 2013.
Malathi VM, Jalali SK, Lyju VJ, Gracy RG, More RP, Anandham R, Thulasi A, Venkatesan T (2017) Associated bacterial diversity of insecticide-susceptible and-resistant brown planthopper, Nilaparvata lugens (Homoptera: Delphacidae) analyzed by culture-dependent and-independent methods. Phytoparasitica 45:683–693. https://doi.org/10.1007/s12600-017-0629-3
Mani M, Krishnamoorthy A, Venugopalan R (2004) Role of the aphelinid parasitoid Encarsia guadeloupae in the suppression of the exotic spiralling whitefly Aleurodicus dispersus on banana in India. Biocontrol Sci Technol 14(6):619–622
Martin JH (2004) Whiteflies of Belize (Hemiptera: Aleyrodidae). Part 1-introduction and account of the subfamily Aleurodicinae Quaintance & Baker. Moscasblancas de Belice (Hemiptera: Aleyrodidae). Parte 1-introducción y descripción de la subfamilia Aleurodicinae Quaintance & Baker. Zootaxa 681:1–119
Merzendorfer H, Zimoch L (2003) Chitin metabolism in insects: structure, function and regulation of chitin synthases and chitinases. J Exp Biol 206:4393–4412. https://doi.org/10.1242/jeb.00709
Milagres AM, Machuca A, Napoleao D (1999) Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. J Microbiol Methods 37:1–6. https://doi.org/10.1016/s0167-7012(99)00028-7
Nieder R, Benbi DK, Reichl FX (2018) Microelements and their role in human health in Soil Components and Human Health. Springer, Dordrecht, pp 317–348
Okongo RN, Puri AK, Wang Z, Singh S, Permaul K (2019) Comparative biocontrol ability of chitinases from bacteria and recombinant chitinases from the thermophilic fungus Thermomyces lanuginosus. J Biosci Bioeng 127:663–671. https://doi.org/10.1016/j.jbiosc.2018.11.007
Palaniswami MS, Pillai KS, Nair RR, Mohandas C (1995) A new cassava pest in India. Cassava Newslett 19:6–7
Rodriguez-Kabana R, Godoy G, Morgan-Jones G, Shelby RA (1983) The determination of soil chitinase activity: conditions for assay and ecological studies. Plant Soil 75(1):95–106
Selvaraj K, Gupta A, Venkatesan T, Jalali SK, Sundararaj R, Ballal CR (2017) First record of invasive rugose spiraling whitefly Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) along with parasitoids in Karnataka. J Biol Control 31:74–78. https://doi.org/10.18311/jbc/2017/16015
Spss (2013) IBM SPSS statistics 22, IBM Corp New York.
Sreerag RS, Jayaprakas CA, Ragesh L, Kumar SN (2014) Endosymbiotic bacteria associated with the mealy bug, Rhizoecus amorphophalli (Hemiptera: Pseudococcidae). Int SchRes Notices. https://doi.org/10.1155/2014/268491
Stocks IC, Hodges G (2012) The rugose spiraling whitefly, Aleurodicus rugioperculatus Martin, a new exotic whitefly in South Florida (Hemiptera: Aleyrodidae), Florida Department of Agriculture and Consumer Services, Division of Plant Industry, Aleurodicus rugioperculatus. Pest-alert. DACS-P-01745 pdf
Sundararaj R, Selvaraj K (2017) Invasion of rugose spiraling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae): a potential threat to coconut in India. Phytoparasitica 45:71–74. https://doi.org/10.1007/s12600-017-0567-0
Taravati S, Mannion C, Glenn H, Osborne L (2013) Natural Enemies of Rugose Spiraling Whitefly, Aleurodicus rugioperculatus Martin (Insecta: Hemiptera: Aleyrodidae) in the South Florida Landscape. ENY-870/IN1004. EDIS. Accessed Aug. 31 2013.
Wen HC, Hsu TC, Chen CN (1995) Yield loss and control of spiralling whitefly (Aleurodicus dispersus Russell). J Agric Res China 44:147
Wiwat C, Thaithanun S, Pantuwatana S, Bhumiratana A (2000) Toxicity of chitinase-producing Bacillus thuringiensiss sp. kurstaki HD-1 (G) toward Plutella xylostella. J Invertebr Pathol 76:270–277. https://doi.org/10.1006/jipa.2000.4976
Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617. https://doi.org/10.1099/ijsem.0.001755
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This work was supported by the Department of Science and Technology, Government of India-New Delhi, under grant GOI-DST (SERB)/EMR/2016/005815.
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Saranya, M., Kennedy, J.S., Anandham, R. et al. Characterization and functional significance of bacteria associated with rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) reared on guava plants. Appl Entomol Zool 57, 323–331 (2022). https://doi.org/10.1007/s13355-022-00791-9
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DOI: https://doi.org/10.1007/s13355-022-00791-9