Abstract
Sweet corn possessing recessive shrunken2 (sh2) gene is popular worldwide. Traditional sweet corn is poor in vitamin A and vitamin E. Plant breeders during the selection of sweet corn genotypes mainly emphasize on plant architecture and yield. Seed germination and seedling vigour play important role for early establishment in field, thereby increasing yield and income. Here, we analysed a set of 15 sh2-based biofortified sweet corn inbreds with crtRB1 (β-carotene hydroxylase1) and vte4 (γ-tocopherol methyltransferase) genes and three traditional sh2-based sweet corn inbreds for nutritional quality, seed vigour and various physico-biochemical traits. The newly developed inbreds possessed significantly higher provitamin A (proA: 15.60 µg/g) and vitamin E [α-tocopherol (α-T): 20.42 µg/g] than the traditional sweet corn inbreds (proA: 2.51 µg/g, α-T: 11.16 µg/g). The biofortified sweet corn inbreds showed wide variation for germination (80.67–87.33%), vigour index-I (2097.17–2925.28 cm), vigour index-II (134.27–216.19 mg) and electrical conductivity (10.19–13.21 μS cm−1 g−1). Wide variation was also observed for dehydrogenase (1.29–1.59 OD g−1 ml−1), super oxide dismutase (4.01–9.82 g−1), peroxidase (11.66–16.47 μM min−1 g−1), esterase (22.98–34.76 nM min−1 g−1) and α-amylase (5.91–8.15 OD g−1 ml−1). Enrichment of proA and vitamin E in sweet corn did not affect seed vigour and physico-biochemical traits. Correlation analysis revealed that electrical conductivity and α-amylase activity was the reliable indicator for assessing seed germination and vigour. The study identified superior biofortified sweet corn genotypes that would contribute to better vigour and establishment in field. This is the first report of analysis of biofortified sweet corn genotypes for seed vigour and physico-biochemical traits.
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References
Abdul-Baki AA, Anderson JD (1973) Vigor determination in soybean by multiple criteria. Crop Sci 13:630–633. https://doi.org/10.2135/cropsci1973.0011183X001300060013x
Adesanya FO, Fakorede MAB, Ajayi SA, Maji AT, Awosanmi FE, Olisa F (2018) Assessment of germination parameters, accelerated ageing and conductivity tests on seeds of upland rice (Oryza sativa L.). Int J Pure App Biosci 6(5):8–13. https://doi.org/10.18782/2320-6832
Aung UT, Mcdonald MB (1995) Changes in esterase activity associated with peanut (Archis hypogaea L.) Seed deterioration. Seed Sci Technol 23:101–111
de Barros M, Macedo GA (2015) Biochemical characterization of purified esterase from soybean (Glycine max) seed. J Am Oil Chem Soc 92:37–45. https://doi.org/10.1007/s11746-014-2573-4
Baseggio M, Murray M, Magallanes-Lundback M, Kaczmar N, Chamness J, Buckler ES, Smith ME, DellaPenna D, Tracy WF, Gore MA (2019) Genome-wide association and genomic prediction models of tocochromanols in fresh sweet corn kernels. Plant Genome 11:180038. https://doi.org/10.3835/plantgenome2018.06.0038
Baveja A, Muthusamy V, Panda KK, Zunjare RU, Das AK, Chhabra R, Hossain F (2021) Development of multinutrient-rich biofortified sweet corn hybrids through genomics-assisted selection of shrunken2, opaque2, lcyE and crtRB1 genes. J Appl Genet 1-11.https://doi.org/10.1007/s13353-021-00633-4
Bouis H (2018) Reducing mineral and vitamin deficiencies through biofortification: Progress under HarvestPlus. In H. K. Biesalski, & R. Birner (Eds), Hidden hunger: strategies to improve nutrition quality Karger Publishers, Basel Switzerland, Vol 118 pp 112–122
Chander S, Guo YQ, Yang XH, Yan JB, Zhang YR, Song TM, Li JS (2008) Genetic dissection of tocopherol content and composition in maize grain using quantitative trait loci analysis and the candidate gene approach. Mol Breed 22(3):353–365. https://doi.org/10.1007/s11032-008-9180-8
Chauhan HS, Chhabra R, Rashmi T, Muthusamy V, Zunjare RU, Mishra SJ, Gain N, Mehta BK, Singh AK, Gupta HS, Hossain F (2022) Impact of vte4 and crtRB1 genes on composition of vitamin-E and provitamin-A carotenoids during kernel-stages in sweet corn. J Food Compost Anal 105:0889–1575. https://doi.org/10.1016/j.jfca.2021.104264
Cheng X, Xiong F, Wang C, Xie H, He S, Geng G, Zhou Y (2018) Seed reserve utilization and hydrolytic enzyme activities in germinating seeds of sweet corn. Pak J Bot 50(1), 111–116. http://www.pakbs.org/.../1531399104.pdf.
Chhabra R, Muthusamy V, Nisrita G, Katral A, Prakash NR, Zunjare RU, Hossain F (2021) Allelic variation in sugary1 gene affecting kernel sweetness among diverse mutant- and wild- type maize inbreds. Mol. Genet. Genom. https://doi.org/10.1007/s00438-021-01807-9.
Dadlani M, Agrawal PK (1987) Techniques in seed science and technology. Seed Technology. South Asian Publishers, New Delhi, pp 103–104
Das AK, Gowda MM, Muthusamy V, Zunjare RU, Chauhan HS, Baveja A, Bhatt V, Chand G, Bhat JS, Guleria SK, Saha S, Gupta HS, Hossain F (2021) Development of maize hybrids with enhanced vitamin-E, vitamin-A, lysine and tryptophan through molecular breeding. Frontiers in Plant Sci. https://doi.org/10.3389/fpls.2021.659381.
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101. https://doi.org/10.1093/jxb/32.1.93
Feng F, Wang Q, Liang C, Yang R, Li X (2015) Enhancement of tocopherols in sweet corn by marker-assisted backcrossing of ZmVTE4. Euphytica 206:513–521. https://doi.org/10.1007/s10681-015-1519-8
França-Neto JDB, Krzyzanowski FC (2019) Tetrazolium: an important test for physiological seed quality evaluation. J Seed Sci 41(3):359–366. https://doi.org/10.1590/2317-1545v41n3223104
Franco OL, Rigden DJ, Melo FR, Grossi-De-Sá MF (2002) Plant α-amylase inhibitors and their interaction with insect α-amylases. Eur J Biochem 269:397–412. https://doi.org/10.1046/j.0014-2956.2001.02656.x
Han Z, Ku L, Zhang Z, Zhang J, Guo S, Liu H, Chen Y (2014) QTLs for seed vigor-related traits identified in maize seeds germinated under artificial aging conditions. PLoS One 9(3):e92535. https://doi.org/10.1371/journal.pone.0092535
Hanumanthappa D, Vasudevan SN, Shakuntala NM, Muniswamy M, Kisan B, Macha IS (2018) Role of seed-Zn content on seed longevity of pigeon pea genotypes. Indian J Agric Res 52(3):250–256. https://doi.org/10.18805/IJARe.A-4891
Hossain F, Nepolean T, Vishwakarma AK, Pandey N, Prasanna BM, Gupta HS (2015) Mapping and validation of microsatellite markers linked to sugary1 and shrunken2 genes in maize (Zea mays L.). J Plant Biochem Biotechnol 24(2):135–142
ISTA (2011) Annual Meeting, Zurich, Switerland. ISTA News Bulletin No. 142
Jin J, Long W, Wang L, Liu X, Pan G, Xiang W, Li N, Li S (2018) QTL mapping of seed vigor of backcross inbred lines derived from Oryza longistaminata under artificial aging. Front Plant Sci 9:1909. https://doi.org/10.3389/fpls.2018.01909
Kato-Noguchi H, Macías FA (2005) Effects of 6-methoxy-2-benzoxazolinone on the germination and α-amylase activity in lettuce seeds. J Plant Physiol 162:1304–1307. https://doi.org/10.1016/j.jplph.2005.03.013
Kittock DC, Law AG (1968) Relationship of seedlings vigor, respiration and tetrazolium chloride reduction by germination of Wheat seeds. J Agron 60:286–288. https://doi.org/10.2134/agronj1968.00021962006000030012x
Kravic N, Andelkovic V, Hadzi-Taskovic-Sukalovic V, Vuletic M (2009) Antioxidant activity in seeds of maize genotypes with different percentage of exotic germplasm. Genetika 41(1):21–28. http://rik.mrizp.rs/handle/123456789/290
Kurilich AC, Juvik JA (1999) Quantification of carotenoid and tocopherol antioxidants in Zea mays. J Agric Food Chem 47:1948–1955. https://doi.org/10.1021/jf981029d
Li Q, Yang X, Xu S, Cai Y, Zhang D, Han Y, Li L, Zhang Z, Gao S, Li J, Yan J (2012) Genome-wide association studies identified three independent polymorphisms associated with α-tocopherol content in maize kernels. PLoS One 7(5):e36807. https://doi.org/10.1371/journal.pone.0036807
Mansouri-Far C, Goodarzian-Ghahfarokhi M, Saeidi M, Abdoli M (2015) Antioxidant enzyme activity and germination characteristics of different maize hybrid seeds during ageing. EEB. 13: 177–182. http://eeb.lu.lv/EEB/201512/EEB_13_Ma...
Marcos-Filho J (2015) Seed vigor testing: an overview of the past, present and future perspective. Sci Agric 72(4):363–374. https://doi.org/10.1590/0103-9016-2015-0007
Mehta BK, Muthusamy V, Baveja A, Chauhan HS, Chhabra R, Bhatt V, Hossain F (2020) Composition analysis of lysine, tryptophan and provitamin-A during different stages of kernel development in biofortified sweet corn. J Food Compost Anal 94:103625. https://doi.org/10.1016/j.jfca.2020.103625
Oliveira TLD, Pinho RGV, Santos HOD, Silva KMDJ, Pereira EDM, Souza JLD (2020) Biochemical changes and physiological quality of corn seeds subjected to different chemical treatments and storage times. J Seed Sci 42.https://doi.org/10.1590/2317-1545v42234181
Pairochteerakul P, Jothityangkoon D, Ketthaisong D, Simla S, Lertrat K, Suriharn B (2018) Seed germination in relation to total sugar and starch in endosperm mutant of sweet corn genotypes. Agronomy 8(12):299. https://doi.org/10.3390/agronomy8120299
Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet-B- and ozone induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136. https://doi.org/10.1104/pp.110.1.125
Rosental L, Nonogaki H, Fait A (2014) Activation and regulation of primary metabolism during seed germination. Seed Sci Res 24(1):1–15. https://doi.org/10.1017/S0960258513000391
Saha S, Walia S, Kundu A, Pathak N (2013) Effect of mobile phase on resolution of the isomers and homologues of tocopherols on a triacontyl stationary phase. Anal Bioanal Chem 405(28):9285–9295. https://doi.org/10.1007/s00216-013-7336-9
Soltani A, Gholipoor M, Zeinali E (2006) Seed reserve utilisation and seedling growth of wheat as affected by drought and salinity. Environ Exp Bot 55:195–200. https://doi.org/10.1016/j.envexpbot.2004.10.012
Wilson DO Jr, Alleyne JC, Shafii B, Mohan SK (1992) Combining vigour test results for prediction of final stand of shrunken-2 sweet corn seed. Crop Sci 32:1496–1502. https://doi.org/10.2135/cropsci1992.0011183X003200060038x
Yan J, Kandianis CB, Harjes CE, Bai L, Kim EH, Yang X, Skinner DJ, Fu Z, Mitchell S, Li Q, Fernandez MGS (2010) Rare genetic variation at Zea mays crtRB1 increases β-carotene in maize grain. Nat Genet 42:322–327. https://doi.org/10.1038/ng.551
Zhao G, Sun Q, Wang J (2007) Improving seed vigour assessment of super sweet and sugar-enhanced sweet corn (Zea mays saccharata). N Z J Crop Hortic Sci 35(3):349–356. https://doi.org/10.1080/01140670709510201
Zunjare RU, Hossain F, Muthusamy V, Baveja A, Chauhan HS, Bhat JS, Thirunavukkarasu N, Saha S, Gupta HS (2018) Development of biofortified maize hybrids through marker-assisted stacking of β-carotene hydroxylase, lycopene-ε-cyclase and opaque2 genes Front. Plant Sci 9:178. https://doi.org/10.3389/fpls.2018.00178
Acknowledgements
IARI, New Delhi is duly acknowledged for providing the field and lab facilities for undertaking the work.
Funding
The funding of DBT-Network project on “Enrichment of nutritional quality in maize through molecular breeding” (BT/PR10922/AGII/106/944/2014) and ICAR-Consortia Research Platform on “Molecular breeding for improvement of tolerance to biotic and abiotic stresses, yield and quality traits in crops—Maize component” (IARI Project Code No.: 12-143C), in supporting the study is duly acknowledged.
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Conduct of the experiment: HSC. Development and maintenance of the inbreds: VM. Analysis of seed vigour traits: SB. Analysis of physico-biochemical traits: AA. Selfing of sweet corn inbreds and sample preparation: BKM. Estimation of carotenoids and tocopherols: NG. Statistical analysis: RUZ. Drafting of manuscript: HSC, BKM and FH. Design of experiment: FH, TR, AKS and HSG. Generation of fund: FH, HSG and AKS.
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Communicated by: Izabela Pawłowicz
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Chauhan, H.S., Muthusamy, V., Rashmi, T. et al. Characterization of crtRB1- and vte4-based biofortified sweet corn inbreds for seed vigour and physico-biochemical traits. J Appl Genetics 63, 651–662 (2022). https://doi.org/10.1007/s13353-022-00715-x
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DOI: https://doi.org/10.1007/s13353-022-00715-x