Abstract
Glycosyltransferase gene family 1, also known as uridine diphosphate glycosyltransferase (UGT), is the largest glycosyltransferase family in plants, playing a vital role in their growth and development. In this study, 244 UGT genes with conserved PSPG motifs were identified in the genome of Quercus robur L. The collinearity analysis results showed that tandem repeat was the main way of UGT genes expansion in Q. robur, with 21 groups of 55 tandem repeat genes. UGT genes were divided into 15 subgroups A–P; group K was lost, and the gene structure and conserved domain of the same subgroup were basically the same. Cis-element analysis showed that upstream 2,000 bp promoter sequence of UGT genes contained light response elements, plant hormone response elements, and stress-related cis-elements, which indicated that UGT genes of Q. robur might be regulated by various metabolic pathways. In particular, some UGTs in group L of Q. robur contained a conserved promoter structure. The expression pattern analysis results demonstrated that UGT genes of groups B, D, E, and I were differentially expressed under Tortrix viridana L. stress. The expression of UGTs in group E decreased under stress, the expression of group L increased, and that of genes in groups D and B were different. The functions of UGT genes in E and L groups are relatively conservative, and their functions may also conserve among species. The study results have a particular reference value for further research on the function of Q. robur UGT genes.
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The project was supported by grants from Natural Science Foundation of Hebei Province (H2020209033) and Hebei Education Department sponsored scientific research projects (ZD2019075).
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The first draft of the manuscript was written by Jie Zhang and all authors commented on previous versions of the manuscript. Material preparation, data collection, and analysis were performed by Li-Mei Lin and Jie Zhang. Zhao-Bin Xing contributed to the study conception and design. Wen-Wen Cheng, Xin-Song, and Yue-Hong Long reviewed and critically revised the manuscript.
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Communicated by: Izabela Pawłowicz
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Zhang, J., Lin, LM., Cheng, WW. et al. Genome-wide identification and expression analysis of glycosyltransferase gene family 1 in Quercus robur L.. J Appl Genetics 62, 559–570 (2021). https://doi.org/10.1007/s13353-021-00650-3
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DOI: https://doi.org/10.1007/s13353-021-00650-3