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Identification of major quantitative trait loci for root diameter in synthetic hexaploid wheat under phosphorus-deficient conditions

  • Plant Genetics • Original Paper
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Abstract

Synthetic hexaploid wheat (SHW) possesses numerous genes for resistance to stress, including phosphorus (P) deficiency. Root diameter (RDM) plays an important role in P-deficiency tolerance, but information related to SHW is still limited. Thus, the objective of this study was to investigate the genetic architecture of RDM in SHW under P-deficient conditions. To this end, we measured the RDM of 138 F9 recombinant inbred lines derived from an F2 population of a synthetic hexaploid wheat line (SHW-L1) and a common wheat line (Chuanmai32) under two P conditions, P sufficiency (PS) and P deficiency (PD), and mapped quantitative trait loci (QTL) for RDM using an enriched high-density genetic map, containing 120,370 single nucleotide polymorphisms, 733 diversity arrays technology markers, and 119 simple sequence repeats. We identified seven RDM QTL for P-deficiency tolerance that individually explained 11–14.7% of the phenotypic variation. Five putative candidate genes involved in root composition, energy supply, and defense response were predicted. Overall, our results provided essential information for cloning genes related to P-deficiency tolerance in common wheat that might help in breeding P-deficiency-tolerant wheat cultivars.

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Acknowledgements

This study was supported by the National Key Research and Development Program of China (2016YFD0101004), the Outstanding Youth Foundation of the Department of Science and Technology of Sichuan Province (2016JQ0040), the Key Technology Research and Development Program of the Department of Science and Technology of Sichuan Province (2016NZ0057), the International Science & Technology Cooperation Program of the Bureau of Science and Technology of Chengdu China (No. 2015DFA306002015-GH03-00008-HZ).

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Wenjiang, 611130, China

    Fangkun Wu, Xilan Yang, Zhiqiang Wang, Mei Deng, Jian Ma, Guoyue Chen, Yuming Wei & Yaxi Liu

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  1. Fangkun Wu
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  2. Xilan Yang
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  7. Yuming Wei
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  8. Yaxi Liu
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Contributions

FW conducted data analysis and drafted the manuscript.

XY, ZW, and MD performed the phenotypic evaluation and help to data analysis.

JM helped to analyze QTL mapping.

GC performed part of population construction.

YW participated in the design of the study.

YL designed and coordinated this study and revised the manuscript.

All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Yaxi Liu.

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The authors declare that they have no competing interests.

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Communicated by: Barbara Naganowska

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Wu, F., Yang, X., Wang, Z. et al. Identification of major quantitative trait loci for root diameter in synthetic hexaploid wheat under phosphorus-deficient conditions. J Appl Genetics 58, 437–447 (2017). https://doi.org/10.1007/s13353-017-0406-5

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  • DOI: https://doi.org/10.1007/s13353-017-0406-5

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