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Two patients with α-chain hemoglobin variant Hb Q-Iran detected by measuring hemoglobin A1c using the variant mode of the HA-8180V HPLC analyzer

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Abstract

Hemoglobin variants are often discovered when hemoglobin A1c (HbA1c) levels measured with a high-performance liquid chromatography (HPLC) system in fast mode are found to be low. The HA-8180V HPLC analyzer by Arkray offers two measurement modes: fast mode (FM) and variant mode (VM). Two Japanese patients with α-chain variant Hb Q-Iran detected incidentally after analyses with the HA-8180V in VM showed an abnormal peak, are presented. The first patient was a man in his 70 s, and the second patient was a man in his 50 s. Both were non-diabetic, but their results from HbA1c measurement in VM showed an abnormal peak. The VM–HbA1c, FM–HbA1c, and HbA1c measured by enzymatic assay and glycated albumin levels of the two patients were all within the reference ranges. They were diagnosed as having Hb Q-Iran (α2-75Asp → His) by globin gene analysis. It is difficult to detect α-chain hemoglobin variants based on abnormal FM–HbA1c levels, but measuring HbA1c in VM is useful for efficiently detecting hemoglobin variants.

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References

  1. American Diabetes Association. Glycemic targets: standards of medical care in diabetes—2022. Diabetes Care. 2022;45(Suppl 1):S83–96.

    Article  Google Scholar 

  2. Koga M. Glycated albumin; clinical usefulness. Clin Chim Acta. 2021;433:96–104.

    Article  Google Scholar 

  3. Bry L, Chen PC, Sacks DB. Effects of hemoglobin variants and chemically modified derivatives on assays for glycohemoglobin. Clin Chem. 2001;47:153–63.

    Article  CAS  PubMed  Google Scholar 

  4. Miyazaki A, Kohzuma T, Kasayama S, et al. Classification of variant forms of haemoglobin according to the ratio of glycated haemoglobin to glycated albumin. Ann Clin Biochem. 2012;49:441–4.

    Article  CAS  PubMed  Google Scholar 

  5. Lorkin PA, Charlesworth D, Lehmann H, et al. Two haemoglobins Q, alpha-74 (EF3) and alpha-75 (EF4) aspartic acid to histidine. Br J Haematol. 1970;19:117–25.

    Article  CAS  PubMed  Google Scholar 

  6. Yadav AK. Comparative analysis of protein structure of common Hb Q variants. Indian J Pathol Microbiol. 2010;53:696–8.

    Article  PubMed  Google Scholar 

  7. Wajcman H. Human hemoglobin variants, the electric versions. http://globin.cse.psu.edu. Accessed 1 July 2023.

  8. Panyasai S, Pornprasert S. Hemoglobin Q-Thailand and its combinations with other forms of thalassemia or hemoglobinopathies in northern Thailand. Clin Lab. 2014;60:1099–103.

    Article  CAS  PubMed  Google Scholar 

  9. Rahimi Z, Muniz A, Mozafari H. Abnormal hemoglobins among Kurdish population of Western Iran: hematological and molecular features. Mol Biol Rep. 2010;37:51–7.

    Article  CAS  PubMed  Google Scholar 

  10. Koga M, Murai J, Soga S, et al. Study on five patients with variant hemoglobin discovered based on abnormally low HbA1c values during routine medical examinations. J Japan Diab Soc. 2013;56:841–8.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Laboratory Medicine, Tenri Hospital, Nara, Japan

    Maki Kinoshita, Daiki Shimomura, Masashi Shimada & Mikio Kamioka

  2. Department of Internal Medicine, Hakuhokai Central Hospital, 4-23-1 Higashisonodacho, Amagasaki, Hyogo, 661-0953, Japan

    Masafumi Koga

Authors
  1. Maki Kinoshita
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  2. Daiki Shimomura
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  3. Masashi Shimada
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  4. Mikio Kamioka
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  5. Masafumi Koga
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Correspondence to Masafumi Koga.

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All procedures were followed in accordance with the ethical standards of the responsible committee on human experimentation (the ethics committee of Tenri Hospital [approval number of Ethics Committee: 937-2, date of approval: October 20, 2021]) and with the Helsinki Declaration of 1964 and later versions. Informed consent or a substitute for it was obtained from all patients prior to their participation in this study.

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Kinoshita, M., Shimomura, D., Shimada, M. et al. Two patients with α-chain hemoglobin variant Hb Q-Iran detected by measuring hemoglobin A1c using the variant mode of the HA-8180V HPLC analyzer. Diabetol Int 15, 302–306 (2024). https://doi.org/10.1007/s13340-023-00682-6

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  • DOI: https://doi.org/10.1007/s13340-023-00682-6

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