Skip to main content
SpringerLink
Log in

Distribution, molecular characterization and diversity of banana bunchy top virus in Tripura, India

  • Original Article
  • Published:
VirusDisease Aims and scope Submit manuscript

Abstract

Banana bunchy top virus (BBTV) is a serious threat to banana (Musa spp.) production in India. Generally, BBTV isolates within the country share very low genetic diversity. However, in India, relatively greater diversity has been observed between isolates from north-eastern (NE) region (Meghalaya) and rest of India. Tripura is situated in the south-west corner of NE India and shares international border with Bangladesh. During 2014–2015, diagnostic surveys were conducted in seven districts of Tripura and polymerase chain reaction based detection established that BBTD is widely prevalent in all parts of Tripura showing an average incidence of 22.02%. Among the cultivars, maximum BBTV infection (27.03%) was recorded in ‘Chini Champa’, followed by plantain (24.29%). A representative population (31 isolates) of BBTV from Tripura was characterized based on DNA R and DNA S. Phylogenetic analysis based on BBTV DNA R and DNA S generated two broad clusters of Pacific-Indian Oceans (PIO) and south-east Asian groups including all Tripura isolates within PIO cluster. The clustering pattern and genetic diversity of BBTV population from Tripura suggested monophyletic origin of majority of representative isolates from a common ancestor of PIO group. The exchange of vegetative propagules within and in between countries could have contributed to the geographical expansion of PIO isolates in Tripura. However, four variant BBTV isolates has been identified from North Tripura and Khowai districts possessing somewhat unique variability than that of distinct isolate (BBTV-Umiam) reported from NE India (Meghalaya).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Anhalt MD, Almeida RPP. Effect of temperature, vector life stage, and plant access period on transmission of Banana bunchy top virus to banana. Phytopathology. 2008;98:743–8.

    Article  PubMed  CAS  Google Scholar 

  2. Banerjee A, Roy S, Behere GT, Roy SS, Dutta SK, Ngachan SV. Identification and characterization of a distinct Banana bunchy top virus isolate of Pacific-Indian Oceans group from North-East India. Virus Res. 2014;183:41–9.

    Article  PubMed  CAS  Google Scholar 

  3. Burns TM, Harding RM, Dale JL. The genome organization of Banana bunchy top virus: analysis of six ssDNA components. J Gen Virol. 1995;76:1471–82.

    Article  PubMed  CAS  Google Scholar 

  4. Chiaki Y, Nasir N, Herwina H, Sonoda A, Fukumoto T, Nakamura M, Iwai H. Genetic structure and diversity of the Banana bunchy top virus population on Sumatra Island, Indonesia. Eur J Plant Pathol. 2015;143:113–22.

    Article  Google Scholar 

  5. Dale JL. Banana bunchy top: an economically important tropical plant virus disease. Adv Virus Res. 1987;33:301–26.

    Article  PubMed  CAS  Google Scholar 

  6. Drew RA, Moisander JA, Smith MK. The transmission of Banana bunchy top virus in micropropagated bananas. Plant Cell Tissue Organ Cult. 1989;16:187–93.

    Google Scholar 

  7. Hanley-Bowdoin L, Settlage SB, Orozco BM, Nagar S, Robertson D. Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Biochem Mol Biol. 2000;35:105–40.

    PubMed  CAS  Google Scholar 

  8. Herrera-Valencia VA, Dugdale B, Harding RM, Dale JL. An iterated sequence in the genome of Banana bunchy top virus is essential for efficient replication. J Gen Virol. 2006;87:3409–12.

    Article  PubMed  CAS  Google Scholar 

  9. Horser CL, Harding R, Dale J. Banana bunchy top nanovirus DNA-1 encodes the ‘master’ replication initiation protein. J Gen Virol. 2001;82:459–64.

    Article  PubMed  CAS  Google Scholar 

  10. Hurst LD. The Ka/Ks ratio: diagnosing the form of sequence evolution. Trends Genet. 2002;18:486–7.

    Article  PubMed  Google Scholar 

  11. Jose PC. Reaction of different varieties of banana against bunchy top disease. Agric Res J Kerala. 1981;1981(19):108–10.

    Google Scholar 

  12. Karan M, Harding RM, Dale JL. Evidence for two groups of Banana bunchy top virus isolates. J Gen Virol. 1994;75:3541–6.

    Article  PubMed  CAS  Google Scholar 

  13. King AM, Adams MJ, Lefkowitz EJ, Carstens EB, Ball LA. Virus taxonomy: IXth report of the international committee on taxonomy of viruses. London: Elsevier Academic Press; 2011.

    Google Scholar 

  14. Kumar PL, Hanna R, Alabi OJ, Soko MM, Oben TT, Vangu GHP, Naidu RA. Banana bunchy top virus in sub-Saharan Africa: investigations on virus distribution and diversity. Virus Res. 2011;159(2):171–82.

    Article  PubMed  CAS  Google Scholar 

  15. Kumar PL, Selvarajan R, Iskra-Caruana ML, Chabannes M, Hanna R. Biology, etiology, and control of virus diseases of banana and plantain. Adv Virus Res. 2015;91:229–69.

    Article  PubMed  Google Scholar 

  16. Magee CJP. Transmission studies on the banana bunchy-top virus. J Aust Inst Agric Sci. 1940;6:109–10.

    Google Scholar 

  17. Molina AB, Kudagamage C. The international network for the improvement of banana and plantain (INIBAP): PGR activities in South Asia. In: South Asia network on plant genetic resources (SANPGR) meeting held on December 9–11 at Plant Genetic Resources Center (PGRC), Peradeniya, Sri Lanka; 2002. pp. 1–7.

  18. Rozas J, Sanchez-DelBarrio JC, Messeguer X, Rozas R. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 2003;19:2496–7.

    Article  PubMed  CAS  Google Scholar 

  19. Rybicki EP. A top ten list for economically important plant viruses. Arch Virol. 2015;160:17–20.

    Article  PubMed  CAS  Google Scholar 

  20. Selvarajan R, Mary Sheeba M, Balasubramanian V, Rajmohan R, Lakshmi Dhevi N, Sasireka T. Molecular characterization of geographically different banana bunchy top virus (BBTV) isolates in India. Indian J Virol. 2010;21(2):110–6.

    Article  PubMed  CAS  Google Scholar 

  21. Stainton D, Martin DP, Muhire BM, Lolohea S, Halafihi M, Lepoint P, Blomme G, Crew KS, Sharman M, Kraberger S, Dayaram A, Walters M, Collings DA, Mabvakure B, Lemey P, Harkins GW, Thomas JE, Varsani A. The global distribution of Banana bunchy top virus reveals little evidence for frequent recent, human-mediated long distance dispersal events. Virus Evol. 2015;1(1):1–16.

    Article  Google Scholar 

  22. Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA. 2004;2004(101):11030–5.

    Article  Google Scholar 

  23. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725–9.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  24. Vishnoi R, Raj SK, Prasad V. Molecular characterization of an Indian isolate of Banana bunchy top virus based on six genomic DNA components. Virus Genes. 2009;38(2):334–44.

    Article  PubMed  CAS  Google Scholar 

  25. Wardlaw CW. Mosaic, Infectious chlorosis and other virus diseases. Banana diseases, including plantains and abaca. London: Longmans; 1961.

    Google Scholar 

  26. Watterson GA. On the number of segregating sites in genetical models without recombination. Theor Popul Biol. 1975;7:256–76.

    Article  PubMed  CAS  Google Scholar 

  27. Wu RY, Su HJ. Purification and characterization of Banana bunchy top virus. J Phytopathol. 1990;125:153–60.

    Article  Google Scholar 

  28. Yu NT, Zhang YL, Feng TC, Wang JH, Kulye M, Yang WJ, Liu ZX. Cloning and sequence analysis of two Banana bunchy top virus genomes in Hainan. Virus Genes. 2012;44:488–94.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work is partially carried out under M.Sc. thesis programme by Tanmoy Das. The authors are thankful to Dr. Satish Chandra, Dr. G. T. Behere and Dr. T. Rajesh for their critical suggestions during the work. The work was supported by IXX09855 project of Indian Council of Agricultural Research.

Author information

Author notes

  1. Amrita Banerjee

    Present address: ICAR National Rice Research Institute, Central Rainfed Upland Rice Research Station, Hazaribag, Jharkhand, 825301, India

Authors and Affiliations

  1. School of Crop Protection, College of Post Graduate Studies, Central Agricultural University, Umiam, Meghalaya, 793103, India

    Tanmoy Das

  2. ICAR Research Complex for North Eastern Hill Region, Umiam, Meghalaya, 793103, India

    Amrita Banerjee

Authors
  1. Tanmoy Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amrita Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amrita Banerjee.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Das, T., Banerjee, A. Distribution, molecular characterization and diversity of banana bunchy top virus in Tripura, India. VirusDis. 29, 157–166 (2018). https://doi.org/10.1007/s13337-018-0451-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13337-018-0451-7

Keywords

Search

Navigation