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Isolation and phylogenetic analysis of caprine Orf virus in Malaysia

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Abstract

Orf virus is a DNA virus that causes contiguous ecthyma in goat and sheep. Infection of animals with this virus cause high mortality in young animals resulting in huge economic losses. In this study, we investigated an outbreak of Orf in a goat farm in Malaysia. Samples were collected from infected animals and viral isolation was done using both LT and MDCK cell lines. Molecular detection was done by conventional PCR for specific primers; B2L and F1L genes and phylogenetic analysis was done on the sequence data obtained. Cytopathic effects (CPE) were observed in both cell lines after 3 days of inoculation and were 50 % by the sixth day. PCR showed positive bands for both B2L and F1L genes and phylogenetic analysis showed that the Malaysian strain had close homology to the Chinese and Indian Orf virus isolates. This study gives more insight into the existing Orf viral strains in Malaysia and their relationship with other strains globally.

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Authors and Affiliations

  1. Institute of Bioscience, University Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia

    Ashwaq Ahmed Abdullah, Zeenatul Allaudin Nazariah, Rasedee Abdullah & Mohd-Azmi Mohd-Lila

  2. Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia

    Muhammad Farid Bin Ismail, Krishnan Nair Balakrishnan, Jamilu Abubakar Bala, Homayoun Hani, Yusuf Abba, Mohd Kamaruddin Awang Isa, Siti Suri Arshad, Zeenatul Allaudin Nazariah, Rasedee Abdullah & Noordin Mohamed Mustapha

  3. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia

    Faez Firdaus Jesse Abdullah

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  1. Ashwaq Ahmed Abdullah
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Correspondence to Ashwaq Ahmed Abdullah or Mohd-Azmi Mohd-Lila.

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Abdullah, A.A., Ismail, M.F.B., Balakrishnan, K.N. et al. Isolation and phylogenetic analysis of caprine Orf virus in Malaysia. VirusDis. 26, 255–259 (2015). https://doi.org/10.1007/s13337-015-0278-4

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  • DOI: https://doi.org/10.1007/s13337-015-0278-4

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