Abstract
The complete RNA3 genome of Cucumber mosaic virus (CMV) was amplified by RT-PCR from three infected gerbera (Gerbera jamesonii) leaf samples exhibiting severe chlorotic mosaic and flower deformation symptoms. The amplicons obtained were cloned sequenced and deposited in GenBank under the accessions JN692495, JX913531 (from cv. Zingaro) and JX888093 (from cv. Silvester). These sequences shared 98–99 % identities to each other and with a strain of CMV-Banana reported from India, and 90–95 % identities with various strains of CMV reported worldwide. Phylogenetic analysis revealed their closest affinity with CMV-Banana strain, and close relationships with several other strains of CMV of subgroup IB. This study provides evidence of subgroup IB CMV causing severe chlorosis and flower deformation in two cultivars (Zingaro and Silvester) of G. jamesonii in India.
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Acknowledgments
Authors are thankful to the Director, CSIR-National Botanical Research Institute, Lucknow for facilities, Indian Council of Medical Research, New Delhi for fellowship to K. K. Gautam and Council of Scientific & Industrial Research, New Delhi for funding as PMSI project number BSC-117.
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Gautum, K.K., Raj, R., Kumar, S. et al. Complete sequence of RNA3 of Cucumber mosaic virus isolates infecting Gerbera jamesonii suggests its grouping under IB subgroup. VirusDis. 25, 398–401 (2014). https://doi.org/10.1007/s13337-014-0216-x
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DOI: https://doi.org/10.1007/s13337-014-0216-x