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Effective detection of Lily Symptomless Virus using the reverse transcription loop–mediated isothermal amplification method

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Abstract

Lily Symptomless Virus (LSV) is prevalent in lily, causing severe economic losses in Lily production. Therefore, it is quite necessary to develop an effective method for detecting LSV. This study aimed to develop a visual Reverse Transcription Loop–Mediated Isothermal Amplification (RT-LAMP) method to detect LSV. The optimum condition was 60 min at 65 °C. The primers designed were highly specific to LSV and did not cross-react with other seven Lily viruses. The sensitivity of RT-LAMP assay for LSV was 0.0001 ng/ul. This RT-LAMP method was rapid, simple, highly specific, and sensitive and helped in the reliable visual detection of LSV in Lily plants.

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References

  • Kim JH, Yoo HN, Bae EH, Jung YT (2012) Development of an indirect ELISA and immunocapture RT-PCR for Lily virus detection. J Microbiol Biotechn 22:1776–1781

    Article  CAS  Google Scholar 

  • Kwon JY, Ryu KH, Choi SH (2013) Reverse transcription polymerase chain reaction-based system for simultaneous detection of multiple lily-infecting viruses. Plant Pathol J 29:338–343

    Article  PubMed  PubMed Central  Google Scholar 

  • Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63

  • Wang R, Wang J, Li J, Wang Y, Xie Z, An L (2007) Comparison of two gel filtration chromatographic methods for the purification of Lily Symptomless Virus. J Virol Methods 139:125–131

    Article  CAS  PubMed  Google Scholar 

  • Wang R, Wang G, Zhao Q, Zhang Y, An L, Wang Y (2010) Expression, purification and characterization of the Lily Symptomless Virus coat protein from Lanzhou isolate. Virol J 7:34

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

Thisrogram, China (Grant No.G2014070203) and agriculture research system of Shanghai, China (Grant No. 201708). These funding bodies had no role in design, collection, and analysis of t work was financially supported by Shanghai agriculture applied technology development phe study and preparation of the manuscript.

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Author notes

  1. Xiao Zhao and Yanan Du contributed equally to this work.

Authors and Affiliations

  1. Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, China

    Xiao Zhao, Yongchun Zhang, Youming Cai, Huanzhen Zhao & Kai Zhao

  2. College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, Shanghai, 200234, China

    Yanan Du, Yan Xu, Xiaorui Fan, Wenjing Wu, Qi Zhang, Xiaoxia Zhang & Wanjing Zhang

  3. School of Life Science, Taizhou University, 1139 Shifu road, Taizhou, 317000, China

    Jieling Liang, Dan Yang & Ziping Zhu

  4. Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, 201106, China

    Kai Zhao

Authors
  1. Xiao Zhao
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  2. Yanan Du
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  3. Yongchun Zhang
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  4. Jieling Liang
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  5. Youming Cai
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  6. Yan Xu
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  7. Xiaorui Fan
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  8. Wenjing Wu
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  9. Qi Zhang
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  10. Xiaoxia Zhang
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  11. Dan Yang
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  12. Ziping Zhu
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  13. Huanzhen Zhao
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  14. Wanjing Zhang
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  15. Kai Zhao
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Corresponding author

Correspondence to Kai Zhao.

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Zhao, X., Du, Y., Zhang, Y. et al. Effective detection of Lily Symptomless Virus using the reverse transcription loop–mediated isothermal amplification method. Australasian Plant Pathol. 48, 373–374 (2019). https://doi.org/10.1007/s13313-019-00638-y

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  • DOI: https://doi.org/10.1007/s13313-019-00638-y

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