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Detection and molecular characterization of a 16SrI-F phytoplasma in potato showing purple top disease in Ecuador

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Abstract

Potato purple top (PPT) disease has been reported worldwide, including Canada, United States, Mexico, Russia and Pakistan. During surveys carried out in a recent epidemic in Ecuador of potato purple top disease phytoplasmas were identified in symptomatic potatoes as well as in some tissue cultured potato shoots by PCR/RFLP analyses and by sequencing and phylogenetic clustering. The majority of the samples resulted positive to phytoplasmas enclosed in the ribosomal subgroup 16SrI-F, for the first time detected in potato and in the American continent. Further studies will allow verification of the transmission and further presence in Ecuador of this phytoplasma associated with purple top disease of potatoes.

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Acknowledgements

This research was authorized by the agreement between the National Institute of Agronomic Research and the Ministry of Environment of Ecuador reference number MAE-DNB-CM-2015-0024, clause 9.2.5. We thank Dr. Charles W. Barnes, Dr. José Ochoa and the anonymous reviewers for helpful comments and suggestions on the manuscript.

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Authors and Affiliations

  1. Estación Experimental Santa Catalina, Departamento Nacional de Biotecnología y Departamento Nacional de Protección Vegetal, Instituto Nacional de Investigaciones Agropecuarias (INIAP), Panamericana Sur km 1, Quito, Ecuador

    Carmen Castillo Carrillo & Johanna Buitrón Bustamante

  2. DipSA, Plant Pathology, Alma Mater Studiorum–University of Bologna, viale G. Fanin, 42, 40127, Bologna, Italy

    Samanta Paltrinieri & Assunta Bertaccini

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  1. Carmen Castillo Carrillo
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  2. Samanta Paltrinieri
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  3. Johanna Buitrón Bustamante
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  4. Assunta Bertaccini
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Correspondence to Carmen Castillo Carrillo.

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Castillo Carrillo, C., Paltrinieri, S., Bustamante, J.B. et al. Detection and molecular characterization of a 16SrI-F phytoplasma in potato showing purple top disease in Ecuador. Australasian Plant Pathol. 47, 311–315 (2018). https://doi.org/10.1007/s13313-018-0557-9

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  • DOI: https://doi.org/10.1007/s13313-018-0557-9

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