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Biocontrol of cereal cyst nematode by Streptomyces anulatus isolate S07

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Abstract

An actinomyce (S07) isolated from a cyst of Heterodera filipjevi was characterised as Streptomyces anulatus by morphological, physiological and biochemical criteria and 16S rRNA analysis. The biocontrol potential of S07 was evaluated against both Heterodera avenae and H. filipjevi. After an initial assay of S07 culture filtrates on egg hatch in vitro and juvenile survival, the effect on these cereal cyst nematodes (CCN) was evaluated in the greenhouse using naturally infested soil and in the field over two consecutive years. The results showed that S07 significantly reduced the population densities of CCN females in wheat, concurrently increasing grain yield. It is concluded that S07 offers potential as a commercial biocontrol agent.

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Acknowledgments

Funding was provided by the Special Research and Development Fund for Public Benefit Agriculture (No. 200903040), Twelfth Five-year Plan on National Science and Technology Project for Food Production (No. 2013BAD07B07) and the Key Deployment Project of the Chinese Academy of Sciences (No. CXJQ120111). We also thank Syngenta (China) Investment Co., Ltd. for providing the nematicide avermectin.

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Authors and Affiliations

  1. Department of Plant Pathology, College of Plant Protection, Henan Agricultural University, Zhengzhou, 450002, China

    J. Zhang, L. M. Wang, Y. H. Li, S. L. Ding, H. X. Yuan & H. L. Li

  2. Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    I. T. Riley

  3. Collaborative Innovation Center of Henan Grain Crops, Zhengzhou, 450002, China

    H. L. Li

  4. National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450002, China

    H. L. Li

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Correspondence to H. L. Li.

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Zhang, J., Wang, L.M., Li, Y.H. et al. Biocontrol of cereal cyst nematode by Streptomyces anulatus isolate S07. Australasian Plant Pathol. 45, 57–64 (2016). https://doi.org/10.1007/s13313-015-0385-0

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  • DOI: https://doi.org/10.1007/s13313-015-0385-0

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