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Identification and genetic diversity of Rosellinia spp. associated with root rot of coffee in Colombia

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Abstract

The genus Rosellinia includes species that cause root rot on a wide range of herbaceous and woody hosts. In Colombia, these fungi cause serious diseases of potato, forest and fruit trees, as well as coffee plants. The aim of this study was to identify isolates of Rosellinia collected from coffee and other hosts using DNA sequence comparisons of the internal transcribed spacer (ITS) region. Pathogenicity tests were conducted on coffee seedlings to confirm the role of the collected species in coffee root disease. Twenty six isolates were obtained and these were grouped into two clades representing R. bunodes and R. pepo. Isolates from Coffea arabica, Hevea brasiliensis, Macadamia integrifolia, Psidium guajava and Theobroma cacao were identified as R. pepo, while R. bunodes was obtained only from coffee plants. Low levels of genetic variability were observed among isolates of the two species. Pathogenicity tests on coffee with R. bunodes resulted in 98 % seedling death in an average of 10 days, while R. pepo killed 54 % of inoculated seedlings in an average of 16 days confirming the compatibility of both species with this host. Pathogen characterization will be useful for further research in disease diagnosis, soil recovery and breeding for resistance.

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Acknowledgments

This study was made possible through the financial support from the Colombian Institute for the Development of Science and Technology “Francisco José de Caldas” COLCIENCIAS; the Colombian National Center of Coffee Research (Cenicafé) and members of the Tree Protection Co-operative Program (TPCP), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa. We thank James Mehl, Prof. Brenda Wingfield, Michael Mbenoum and Tuan Duong for their advice, assistance in the laboratory and with DNA sequence comparisons. Furthermore, we thank Dr. Juan Carlos Herrera (Cenicafé), Professor Jack Rogers (Washington State University) and Dr. Martijn ten Hoopen (CIRAD- FRANCE) for useful discussions and advice.

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Authors and Affiliations

  1. Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Hatfield, Pretoria, 0028, South Africa

    Bertha L. Castro, Jolanda Roux & Michael J. Wingfield

  2. National Coffee Research Center (CENICAFE), Manizales, Colombia, A.A. 2427

    Angela J. Carreño, Narmer F. Galeano & Álvaro L. Gaitán

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  1. Bertha L. Castro
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  2. Angela J. Carreño
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  3. Narmer F. Galeano
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  4. Jolanda Roux
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  6. Álvaro L. Gaitán
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Correspondence to Bertha L. Castro.

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Castro, B.L., Carreño, A.J., Galeano, N.F. et al. Identification and genetic diversity of Rosellinia spp. associated with root rot of coffee in Colombia. Australasian Plant Pathol. 42, 515–523 (2013). https://doi.org/10.1007/s13313-013-0205-3

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